Human-Robot Interaction is an increasingly important topic in both research and industry fields. Since human safety must be always guaranteed and accidental contact with the operator avoided, it is necessary to investigate real-time obstacle avoidance strategies. The transfer from simulation environments, where algorithms are tested, to the real world is challenging from different points of view, e.g., the continuous tracking of the obstacle and the configuration of different manipulators. In this paper, the authors describe the implementation of a collision avoidance strategy based on the potential field method for off-line trajectory planning and on-line motion control, paired with the Motion Capture system Optitrack PrimeX 22 for obstacle tracking. Several experiments show the performance of the proposed strategy in the case of a fixed and dynamic obstacle, disturbing the robot’s trajectory from multiple directions. Two different avoidance modalities are adapted and tested for both standard and redundant robot manipulators. The results show the possibility of safely implementing the proposed avoidance strategy on real systems.