Dynamic photoacoustic imaging of neurovascular coupling in salivary glands

Rich, Laurie J.; Bolookat, Eftekhar Rajab; Seshadri, Mukund

doi:10.1016/j.job.2019.08.002

Cited by 2 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…No change in parotid gland hemodynamics (HbT/%sO 2 ) was observed in control rabbits between the two imaging sessions (d0 and d8), highlighting the reliability of PAI. These results, along with our published studies in mice [16][17][18], confirm the ability of PAI to reliably measure salivary gland hemodynamics.…”

Section: Discussionsupporting

confidence: 85%

“…While US relies on differences in acoustic impedance between tissues [13], PAI exploits the optical absorption characteristics of endogenous chromophores such as hemoglobin (Hb) to provide quantitative measures of the hemoglobin concentration (HbT) and oxygen saturation (%sO 2 ) [14,15]. Our studies in murine models have validated US-PAI measures of salivary gland hemodynamics with histology and demonstrated the strong correlation between a vascular response to parasympathetic stimulation or blockade and saliva secretion [16,17]. Recently, we have also examined the ability of US-PAI to monitor the early and late vascular response of murine salivary glands to radiation [18].…”

Section: Introductionmentioning

confidence: 84%

See 1 more Smart Citation

Ultrasound-Guided Photoacoustic Imaging of Salivary Gland Hemodynamics in Rabbits

Bolookat,

Vincent-Chong,

Rich

et al. 2024

Photonics

Self Cite

View full text Add to dashboard Cite

Xerostomia (severe dry mouth) is a debilitating and often permanent side effect experienced by head and neck cancer patients due to radiation injury to salivary glands. In this study, we evaluated the potential of ultrasound (US)-guided photoacoustic imaging (PAI) to non-invasively assess early changes in salivary gland hemodynamics following radiation therapy (RT). US-guided PAI was performed in New Zealand white rabbits to visualize and quantify the hemoglobin concentration (HbT) and oxygen saturation (%sO2) of parotid glands before and after RT. The imaging findings were validated with histology and sialometry. An early increase in parotid gland HbT and %sO2 was seen following RT. Consistent with the PAI observations, histology of salivary glands revealed dilated blood vessels, along with hemorrhaging and fibrosis. Sialometric analysis confirmed a significant reduction in stimulated saliva secretion in irradiated rabbits compared to controls. Collectively, our findings demonstrate the translational utility of US-guided PAI as a valuable tool for label-free functional imaging of salivary gland hemodynamics in vivo.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 84%

Ultrasound-Guided Photoacoustic Imaging of Salivary Gland Hemodynamics in Rabbits

Bolookat,

Vincent-Chong,

Rich

et al. 2024

Photonics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Experimental imaging of the murine submandibular–sublingual salivary gland complex was performed using a 21-MHz transducer with built-in fiber-optics (Vevo LAZR VisualSonics) as previously described (Kalmuk et al 2015; Rich, Bolookat, et al 2019). Refer to the Appendix for additional information.…”

Section: Methodsmentioning

confidence: 99%

“…We have previously shown that combined photoacoustic imaging (PAI) and Doppler sonography can provide simultaneous information on salivary gland blood flow and hemodynamics (hemoglobin concentration [HbT] and percent oxygen saturation [%sO 2 ]) in mice (Rich and Seshadri, 2015, 2016). We have recently demonstrated the utility of US-PAI for the assessment of salivary gland vascular response to parasympathetic stimulation (Rich, Bolookat, et al 2019). However, dynamic functional imaging of changes in salivary gland vascularity and oxygenation in response to RT has not been previously reported.…”

Section: Introductionmentioning

confidence: 99%

Noninvasive Monitoring of Radiation-Induced Salivary Gland Vascular Injury

et al. 2022

View full text Add to dashboard Cite

Xerostomia is a common side effect of radiation therapy (RT) in patients with head and neck cancer. However, limited information is available on the temporal dynamics of parenchymal and vascular changes in salivary glands following RT. To address this gap in knowledge, we conducted experimental studies in mice employing ultrasound (US) with coregistered photoacoustic imaging (PAI) to noninvasively assess the early and late changes in salivary gland size, structure, vascularity, and oxygenation dynamics following RT. Multiparametric US-PAI of salivary glands was performed in immune-deficient and immune-competent mice before and after RT along with correlative sialometry and ex vivo histologic-immunohistochemical validation. US revealed reduction in gland volume and an early increase in vascular resistance postradiation. This was accompanied by a reduction in glandular oxygen consumption on PAI. Imaging data correlated strongly with salivary secretion and histologic evidence of acinar damage. The magnitude and kinetics of radiation response were impacted by host immune status, with immunodeficient mice showing early and more pronounced vascular injury and DNA damage response compared to immunocompetent animals. Our findings demonstrate the ability of noninvasive US-PAI to monitor dynamic changes in salivary gland hemodynamics following radiation and highlight the impact of the host immune status on salivary gland radiation injury.

show abstract

Dynamic photoacoustic imaging of neurovascular coupling in salivary glands

Cited by 2 publications

References 21 publications

Ultrasound-Guided Photoacoustic Imaging of Salivary Gland Hemodynamics in Rabbits

Ultrasound-Guided Photoacoustic Imaging of Salivary Gland Hemodynamics in Rabbits

Noninvasive Monitoring of Radiation-Induced Salivary Gland Vascular Injury

Contact Info

Product

Resources

About