Dynamic photoelectrical regulation of ECM protein and cellular behaviors

Self Cite

Surface potential is rarely investigated as an independent factor in influencing tissue regeneration on the metal surface. In this work, the surface potential on the titanium (Ti) surface was designed to be tailored and adjusted independently, which arises from the ferroelectricity and piezoelectricity of poled poly(vinylidene fluoride-trifluoroethylene) (PVTF). Notably, it is found that such controllable surface potential on the metal surface significantly promotes osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) in vitro as well as bone regeneration in vivo. In addition, the intracellular calcium ion (Ca2+) concentration measurement further proves that such controllable surface potential on the metal surface could activate the transmembrane calcium channels and allow the influx of extracellular Ca2+ into the cytoplasm. That might be the reason for improved osteogenic differentiation of BMSCs and bone regeneration. These findings reveal the potential of the metal surface with improved bioactivity for stimulation of osteogenesis and show great prospects for fabricable implantable medical devices with adjustable surface potential.

Section: Discussionmentioning

confidence: 84%

Accelerated Bone Regeneration on the Metal Surface through Controllable Surface Potential

Lin,

Zhou,

Chen

et al. 2023

Self Cite

“…The results of animal experiments generally confirmed that in comparison to the pure POC scaffold, the POC/10% GATP composite scaffold had a more prominent repair effect on osteochondral defects, emphasizing the key role of GATP in the repair process. This lack of biological activity in nonfunctionalized POC scaffolds makes them inadequate for sustaining osteochondral regeneration; − nevertheless, the addition of GATP enables the scaffold to have a dual-lineage biological activity and free radical scavenging ability, creating a beneficial microenvironment for osteochondral regeneration. Hence, the POC/10% GATP scaffold demonstrates significant potential in promoting cartilage and subchondral bone repair and regeneration.…”

Section: Resultsmentioning

confidence: 99%

Nanoclay Reinforced Integrated Scaffold for Dual-Lineage Regeneration of Cartilage and Subchondral Bone

Yin,

Xia,

Fan

et al. 2024

Tissue engineering is theoretically considered a promising approach for repairing osteochondral defects. Nevertheless, the insufficient osseous support and integration of the cartilage layer and the subchondral bone frequently lead to the failure of osteochondral repair. Drawing from this, it was proposed that incorporating glycine-modified attapulgite (GATP) into poly(1,8octanediol-co-citrate) (POC) scaffolds via the one-step chemical cross-linking is proposed to enhance cartilage and subchondral bone defect repair simultaneously. The effects of the GATP incorporation ratio on the physicochemical properties, chondrocyte and MC3T3-E1 behavior, and osteochondral defect repair of the POC scaffold were also evaluated. In vitro studies indicated that the POC/10% GATP scaffold improved cell proliferation and adhesion, maintained cell phenotype, and upregulated chondrogenesis and osteogenesis gene expression. Animal studies suggested that the POC/10% GATP scaffold has significant repair effects on both cartilage and subchondral bone defects. Therefore, the GATP-incorporated scaffold system with dual-lineage bioactivity showed potential application in osteochondral regeneration.

“…MSCs express high levels of integrins, important components of focal adhesions (FAs), responsible for connecting the extracellular matrix (ECM) to cells, and triggering adhesion and mechanosensing . In other words, manipulating the surface topography of materials interacting with the ECM can modulate the morphology and function of MSCs by altering specificity of integrin recognition . The surface morphology that affects cell fate decisions of MSCs mainly includes three topographical features: roughness, porosity, and surface patterns .…”

Section: Introductionmentioning

confidence: 99%

“…5 In other words, manipulating the surface topography of materials interacting with the ECM can modulate the morphology and function of MSCs by altering specificity of integrin recognition. 6 The surface morphology that affects cell fate decisions of MSCs mainly includes three topographical features: roughness, porosity, and surface patterns. 7 Compared with micro-, nanorange roughness significantly promoted the polarization of M2 macrophages and the osteogenic differentiation of stem cells.…”

Section: Introductionmentioning

confidence: 99%

Microgroove Cues Guiding Fibrogenesis of Stem Cells via Intracellular Force

Lei

Miao

Wang

et al. 2023

Groove patterns are widely used in material surface modifications. However, the independent role of ditches/ridges in regulating fibrosis of soft tissues is not well-understood, especially the lack of linkage evidence in vitro and in vivo. Herein, two kinds of combinational microgroove chips with the gradient ditch/ridge width were fabricated by photolithography technology, termed R and G groups, respectively. In group R, the ridge width was 1, 5, 10, and 30 μm, with a ditch width of 30 μm; in group G, the groove width was 5, 10, 20, and 30 μm, and the ridge width was 5 μm. The effect of microgrooves on the morphology, proliferation, and expression of fibrous markers of stem cells was systematically investigated in vitro. Moreover, thicknesses of fibrous capsules were evaluated after chips were implanted into the muscular pouches of rats for 5 months. The results show that microgrooves have almost no effect on cell proliferation but significantly modulate the morphology of cells and focal adhesions (FAs) in vitro, as well as fibrosis differentiation. In particular, the differentiation of stem cells is attenuated after the intracellular force caused by stress fibers and FAs is interfered by drugs, such as rotenone and blebbistatin. Histological analysis shows that patterns of high intracellular force can apparently stimulate soft tissue fibrosis in vivo. This study not only reveals the specific rules and mechanisms of ditch/ridge regulating stem cell behaviors but also offers insight into tailoring implant surface patterns to induce controlled soft tissue fibrosis.