Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells

Fuchs, Sabine; Jiang, Xuemei; Schmidt, Harald; Dohle, Eva; Ghanaati, Shahram; Orth, Carina; Hofmann, Alexander; Motta, Antonella; Migliaresi, Claudio; Kirkpatrick, Charles James

doi:10.1016/j.biomaterials.2008.11.028

Cited by 151 publications

(117 citation statements)

References 41 publications

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“…In addition, Shh could be beneficial in reducing the effective pre-culture time of prevascularised tissues in vitro. Our previous studies demonstrated that the formation of microvessellike structures of blood-derived outgrowth endothelial cells in co-culture is usually initiated within 1 week of co-culture and proceeds with ongoing culture time in cultures in EGM-2 with no additional growth factors (Fuchs et al, 2007;Fuchs et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

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Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies

Dohle

Fuchs²,

Kolbe³

et al. 2011

eCM

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Section: Discussionmentioning

confidence: 99%

“…Microscopic images of immunofluorescence-stained cocultures in response to different treatments were analysed using ImageJ 1.43 as previously published (Fuchs et al, 2009b …”

Section: Image Quantificationmentioning

confidence: 99%

Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies

Dohle

Fuchs²,

Kolbe³

et al. 2011

eCM

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“…The study is based on an in vitro co-culture model consisting of outgrowth endothelial cells (OECs) and primary osteoblasts (pOBs), which has the potential to permit further insight into underlying mechanisms of bone vascularisation and could also support the identification of factors stimulating the vascularisation process in therapeutic approaches. It has already been shown in a number of studies that OECs form lumen-containing, microvessel-like structures after 3 to 4 weeks of co-cultivation when seeded together with primary osteoblasts (Fuchs et al, 2007;Fuchs et al, 2009). The osteoblasts seem to provide proangiogenic matrix as well as proangiogenic cues that might lead to the angiogenic activation of OECs in long-term cocultures.…”

Section: Introductionmentioning

confidence: 93%

Macrophage-mediated angiogenic activation of outgrowth endothelial cells in co-culture with primary osteoblasts

Dohle

Bischoff²,

Böse³

et al. 2014

eCM

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The successful vascularisation of complex tissue engineered constructs for bone regeneration is still a major challenge in the field of tissue engineering. In this context, co-culture systems of endothelial cells and osteoblasts represent a promising approach to advance the formation of a stable vasculature as well as an excellent in vitro model to identify factors that positively influence bone healing processes, including angiogenesis. Under physiological conditions, the activation phase of angiogenesis is mainly induced by hypoxia or inflammation. Inflammatory cells such as macrophages secrete proinflammatory cytokines and proangiogenic growth factors, finally leading to the formation of new blood vessels. The aim of this study was to investigate if macrophages might positively influence the formation of microvessel-like structures via inflammatory mechanisms in a co-culture system consisting of human outgrowth endothelial cells (OECs) and primary osteoblasts. Treatment of co-cultures with macrophages (induced from THP-1) resulted in a higher number of microvessel-like structures formed by OECs compared to the co-culture. This change correlated with a significantly higher concentration of the proangiogenic VEGF in cell culture supernatants of triple-cultures and was accompanied by an increase in the expression of different proinflammatory cytokines, such as IL-6, IL-8 and TNFα. In addition, the expression of E-selectin and ICAM-1, adhesion molecules which are strongly involved in the interaction between leukocytes and endothelial cells during the process of inflammation was also found to be higher in triple-cultures compared to the double co-cultures, documenting an ongoing proinflammatory stimulus. These results raise the possibility of actively using pro-inflammatory stimuli in a tissue engineering context to accelerate healing mechanisms.

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“…Compared to the traditional value of B. mori as a source of silk, its major role in the near future will be as a biomedical insect contributing to the production of proteins and biomaterials. In fact, silk fibroin-based scaffold materials have already been employed for several tissue-engineering applications in place of synthetic polymers (2). The silkworm can also express high levels of foreign genes through the Baculovirus system.…”

Section: Introductionmentioning

confidence: 99%

Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

Park

Kang²,

Goo³

et al. 2010

BMB Reports

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The Bombyx mori Microarray Database (BmMDB; http://silkworm.swu.edu.cn/microarray) provides information for tissue-specific gene expression by using the whole-genome oligonucleotide microarray in the silkworm. We analyzed the tissue-specific expression patterns in the silk gland, fat body, and midgut five days of fifth instar larvae during the development of B. mori. To verify the tissue-specific expression, analysis was conducted using quantitative Real-time RT-PCR and the highly expressed endogenous Actin RNA as an intrinsic reference. Finally, we confirmed five genes, (sw15872, sw00692, sw20990, sw05300,and sw2250), out of 18 candidates expressed in two different tissues, which was consistent with the data published by Dr. Xiang's group, thereby supporting the BmMDB. Further studies for promoter regions of candidate genes can be applied in creating transgenic silkworms as biomedical insects for use in producing biomaterials, and to serve as well-characterized models for understanding the mechanism for the genetic regulation of tissue-specific development.[BMB reports 2010; 43(7): 480-484]

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Dynamic processes involved in the pre-vascularization of silk fibroin constructs for bone regeneration using outgrowth endothelial cells

Cited by 151 publications

References 41 publications

Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies

Comparative study assessing effects of sonic hedgehog and VEGF in a human co-culture model for bone vascularisation strategies

Macrophage-mediated angiogenic activation of outgrowth endothelial cells in co-culture with primary osteoblasts

Tissue-specific gene expression analysis of silkworm (Bombyx mori) by quantitative real-time RT-PCR

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