Dynamic protein adsorption in microchannels by “stop-flow” and continuous flow

Lionello, Andrea; Josserand, Jacques; Jensen, Henrik; Girault, Hubert H.

doi:10.1039/b506009e

Cited by 37 publications

(37 citation statements)

References 20 publications

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“…The above type of experiment, in which the adsorption equilibrium is reached with a limited sample amount in successive injection steps is known as the stopped-flow method [17]. Our results demonstrate the applicability of the tape channels for stopped-flow experiments and pave the way for more sophisticated binding experiments.…”

Section: / 12mentioning

confidence: 70%

Microfluidic channels laser-cut in thin double-sided tapes: Cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips

Patko

Mártonfalvi

Kovács

et al. 2014

Sensors and Actuators B: Chemical

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A simple, reliable and cost-effective fluidic channel, fabricated by using double-sided pressure-sensitive tapes, is demonstrated here. A laser-cutting method is applied to engrave structures in sheets of the tapes. After peeling off the tape liners, the structures could be easily integrated at room temperature with label-free optical waveguide biochips without further modifications or additional processing steps. It is shown that the well-defined and controllable height of the channels is advantageous for stopped-flow measurements of analyte binding.The easy fabrication of a fully transparent integrated sensor unit -tape cuvette system is also demonstrated for parallel microscopic investigations. The transparent unit was used to on-line monitor the surface adhesion of Salmonella cells on poly-L-lysine-coated biochip surfaces, followed by the straightforward microscopic visualization of the adhered bacterial cells. The material of the double sided tape is stable in aqueous solutions. Furthermore, its material is biocompatible, making it ideal for biological applications. Excellent, stable and reversible bonding of the microstructured tapes to biocompatible plastic and glass is also demonstrated.The simplicity of the fabrication at ambient temperatures makes the developed processes appealing for lab-on-a-chip applications, particularly if the bonded biochips are precious. / 12

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Section: / 12mentioning

confidence: 70%

Microfluidic channels laser-cut in thin double-sided tapes: Cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips

Patko

Mártonfalvi

Kovács

et al. 2014

Sensors and Actuators B: Chemical

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“…Linearization using eq (7) in Figure 5B leads [21,24]. These mentioned values are all lower than that found for PET modified with NPs.…”

Section: Gold Nps Growth Enhances Sensormentioning

confidence: 57%

“…Similarly to previous work [21], we assume that assumption is consistent with semi-infinite linear diffusion and its validity in the case of a microsystem is valid [22,23]. Constants k on and k off represent the rates of adsorption and desorption of the antibody onto the active sites.…”

Section: Gold Nps Growth Enhances Sensormentioning

confidence: 99%

Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalat (PET) Modified with Gold Nanoparticles in Flow Microchannel

Kechadi¹,

Sotta²,

Gamby³

2014

J Flow Chem

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The real-time monitoring of the adsorption kinetic of antibody onto polyethylene terephthalate (PET) modified with gold nanoparticles (NPs) is performed into a dielectric flow microchannel. The principle is based on the microelectrode-dielectric interface excitation by a modulated voltage excitation through the dielectric layer with high frequency range. The set-up configuration using a homemade current-to-voltage converter has been developed for fast monitoring of biomolecule adsorption without direct electrical contact of microelectrodes with the microchannel flow. The change in the interfacial admittance, related to the output voltage measured through the microchip, during antibody incubation, gives information on the kinetics involved while antibodies are adsorbed at the interface. An example of adsorption on PET modified with gold NPS was taken. Experimental data were fit with the Langmuir equation and a good correlation was obtained with this latter, where the equilibrium constant, K, was found as 1.55 × 10 8 M −1 with a limit of detection for antibody concentration without depletion equal to 8.25 nM.

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“…The Langmuir isotherm model has a wide application for the adsorption of proteins on substrates, which is useful in practice. [15] According to the Langmuir adsorption isotherm equation:…”

Section: Wwwchemeurjorgmentioning

confidence: 99%

Assembly‐Controlled Biocompatible Interface on a Microchip: Strategy to Highly Efficient Proteolysis

Liu

Zhong

Meng

et al. 2006

Chemistry A European J

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A biocompatible interface was constructed on a microchip by using the layer‐by‐layer (LBL) assembly of charged polysaccharides incorporating proteases for highly efficient proteolysis. The controlled assembly of natural polyelectrolytes and the enzyme‐adsorption step were monitored by using a quartz‐crystal microbalance and atomic force microscopy (AFM). Such a multilayer‐assembled membrane provides a biocompatible interconnected network with high enzyme‐loading capacity. The maximum digestion rate of the adsorbed trypsin in a microchannel was significantly accelerated to 1600 mM min−1 μg−1, compared with the tryptic digestion in solution. Based on the Langmuir isotherm model, the thermodynamic constant of adsorption K was calculated to be 1.6×105 M−1 and the maximum adsorption loading Γmax was 3.6×10−6 mol m−2, 30 times more than a monolayer of trypsin on the native surface. The tunable interface containing trypsin was employed to construct a microchip reactor for digestion of femtomoles of proteins and the produced peptides were analyzed by MALDI‐TOF mass spectroscopy. The efficient on‐chip proteolysis was obtained within a few seconds, and the identification of biological samples was feasible.

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Dynamic protein adsorption in microchannels by “stop-flow” and continuous flow

Cited by 37 publications

References 20 publications

Microfluidic channels laser-cut in thin double-sided tapes: Cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips

Microfluidic channels laser-cut in thin double-sided tapes: Cost-effective biocompatible fluidics in minutes from design to final integration with optical biochips

Investigating the Kinetics of Antibody Adsorption onto Polyethylene Terephthalat (PET) Modified with Gold Nanoparticles in Flow Microchannel

Assembly‐Controlled Biocompatible Interface on a Microchip: Strategy to Highly Efficient Proteolysis

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