Dynamic Sialylation in Transforming Growth Factor-β (TGF-β)-induced Epithelial to Mesenchymal Transition

Du, Jun; Hong, Senlian; Dong, Lu; Cheng, Bo; Lin, Liang; Zhao, Bing; Chen, Ye-Guang; Chen, Xing

doi:10.1074/jbc.m115.636969

Cited by 56 publications

(48 citation statements)

References 49 publications

(62 reference statements)

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“…Expression of sialylglycans are positively correlated with aggressiveness and metastasis in many cancers. Altered expression of sialylglycans is associated with epithelial-mesenchymal transition (EMT), an essential step for tumor progression and metastasis [53]. Transforming growth factor-β (TGF-β) induced EMT process caused upregulation of various sialyltransferases such as ST3GAL1, ST3GAL2, ST6GAL1, ST6GAL2, ST8SIA1, ST8SIA2, and ST8SIA4 [54][55][56][57][58][59][60][61].…”

Section: Sialic Acids Enhance Tumor Proliferation and Metastasismentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

132

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Sialic acids, a subset of nine carbon acidic sugars, often exist as the terminal sugars of glycans on either glycoproteins or glycolipids on the cell surface. Sialic acids play important roles in many physiological and pathological processes via carbohydrate-protein interactions, including cell–cell communication, bacterial and viral infections. In particular, hypersialylation in tumors, as well as their roles in tumor growth and metastasis, have been widely described. Recent studies have indicated that the aberrant sialylation is a vital way for tumor cells to escape immune surveillance and keep malignance. In this article, we outline the present state of knowledge on the metabolic pathway of human sialic acids, the function of hypersialylation in tumors, as well as the recent labeling and analytical techniques for sialic acids. It is expected to offer a brief introduction of sialic acid metabolism and provide advanced analytical strategies in sialic acid studies.

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Section: Sialic Acids Enhance Tumor Proliferation and Metastasismentioning

confidence: 99%

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Zhou

Yang

Guan

2020

Cells

132

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“…The epithelial‐to‐mesenchymal transition (EMT) is an important cellular event featuring the loss of epithelial cells’ polarity and adhesion and acquisition of mesenchymal cells’ motility and invasiveness . Associated with the induction of EMT (by insulin‐like growth factor 1, or IGF‐1) (Figure S18),, HieCo imaging reveals an increase of FI for both of the MUC1‐bound terminal Sia and Fuc imaging channels (Figures B, S18, and S19), enabling its use as an effective EMT monitoring tool. This observation is also supported by immunoprecipitation (IP) fluorescence analysis (Figure B).…”

Section: Methodsmentioning

confidence: 99%

A Hierarchical Coding Strategy for Live Cell Imaging of Protein‐Specific Glycoform

Liu

et al. 2018

Angewandte Chemie

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Live cell imaging of protein-specific glycoforms holds great promise for revolutionizing the study of glycochemistry.T he imaging protocols developed thus far build upon the paired interplay of probe units,t hus limiting the number of monosaccharide identification channels.Ahierarchical coding (HieCo) imaging strategy,w ith DNAc oding and decoding of protein and monosaccharides executed in fidelity to the hierarchical order of target glycoprotein, is reported herein and features expandable monosaccharide identification channels.Aproof-of-concept protocol has been developed for MUC1-specific imaging of terminal sialic acid (Sia) and fucose (Fuc) on MCF-7, T47D,MDA-MB-231, and PANC-1 cells,r evealing distinct monosaccharide patterns for four types of cells.T he protocol also permits dynamic monitoring of changes in MUC1-specific monosaccharide patterns associated with both the alteration of cellular physiological states and the occurrence of ab iologically important process.Scheme 1. The hierarchicalc oding (HieCo) strategy (A) for live cell imaging of protein-specific glycoform(B).

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“…Reprinted and modified, with permission, from ref. . Copyright: The American Society for Biochemistry and Molecular Biology, 2015.…”

Section: Mgl With Bioorthogonal Chemical Reportersmentioning

confidence: 99%

“…Dynamic changes in sialylation during important cellular processes can also be imaged and monitored by using this strategy. For example, our group recently applied MGL with Ac 4 ManNAz and CuAAC assisted by the BTTAA ligand to visualize dynamic sialylation in epithelial–mesenchymal transition (EMT), a fundamental process in embryonic development and organ formation . It was discovered that sialylation undergoes a downregulation during EMT and is then reverted and upregulated in the mesenchymal state after EMT (Figure B).…”

Section: Mgl With Bioorthogonal Chemical Reportersmentioning

confidence: 99%

“…The treated cells were lysed and treated with streptavidin beads, followed by gel‐based proteomic identification by tandem mass spectrometry. By this chemical glycoproteomics approach, we identified a list of cell‐surface sialylated proteins, the biosynthesis of which was dynamically regulated during EMT, and the relative abundances of individual sialylated glycoproteins between different time points can be quantitatively compared (Figure C) . Similar approaches have been used by several other groups for identifying sialylated proteins in Ac 4 ManNAz‐treated cells .…”

Section: Mgl With Bioorthogonal Chemical Reportersmentioning

confidence: 99%

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Metabolic Remodeling of Cell‐Surface Sialic Acids: Principles, Applications, and Recent Advances

et al. 2015

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Cell-surface sialic acids are essential in mediating a variety of physiological and pathological processes. Sialic acid chemistry and biology remain challenging to investigate, demanding new tools for probing sialylation in living systems. The metabolic glycan labeling (MGL) strategy has emerged as an invaluable chemical biology tool that enables metabolic installation of useful functionalities into cell-surface sialoglycans by "hijacking" the sialic acid biosynthetic pathway. Here we review the principles of MGL and its applications in study and manipulation of sialic acid function, with an emphasis on recent advances.

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Dynamic Sialylation in Transforming Growth Factor-β (TGF-β)-induced Epithelial to Mesenchymal Transition

Abstract: Background: Changes in cell surface sialylation have recently been implicated in mediating epithelial-mesenchymal transition (EMT). Results: Cell surface sialylation was first down-regulated but then reverted and up-regulated during EMT, and inhibition of sialylation promoted EMT.

Cited by 56 publications

References 49 publications

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

Biological Functions and Analytical Strategies of Sialic Acids in Tumor

A Hierarchical Coding Strategy for Live Cell Imaging of Protein‐Specific Glycoform

Metabolic Remodeling of Cell‐Surface Sialic Acids: Principles, Applications, and Recent Advances

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