Dynamic Signature of tRNA-Derived small RNAs in Cancer Pathogenesis as a Promising Valuable Approach

Fattahi, Fahimeh; Sahlolbei, Maryam; Vafaei, Somayeh; Yazdanpanah, Ayna; Kiani, Jafar

doi:10.21203/rs.3.rs-21192/v1

Cited by 2 publications

(2 citation statements)

References 37 publications

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“…The tsRNAs also play a role in pathological and physiological processes, in which gene expression is frequently dysregulated. The tsRNAs bind to Argonaute proteins and Piwi proteins like miRNAs and piRNAs sequentially (Vafaei et al, 2020). In addition, tsRNAs have been detected in archaea.…”

Section: Discussionmentioning

confidence: 99%

Characterization of endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 cells

Jiang¹,

Lin²,

Pu³

et al. 2022

Biocell

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As nucleic acid-guided endonucleases, some prokaryotic Argonautes have been used as programmable nucleases. Natronobacterium gregoryi Argonaute (NgAgo) has also been proposed for gene editing, but this remains very controversial. Until now, the endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 (N. gregoryi sp2) have not been characterized. We expressed the conserved PIWI domain of NgAgo and used it to induce anti-PIWI antibody. We also cultured the N. gregoryi sp2 strain and performed immunoprecipitation, chromatin immunoprecipitation (ChIP), and RNA immunoprecipitation (RIP) assays. The nucleic acids that endogenously bound NgAgo in N. gregoryi sp2 cells were sequenced and analyzed. The results showed that NgAgo endogenously bound RNA rather than DNA. NgAgo-associated RNAs were mainly transcripts of genes that encoded tRNA, transcriptional regulators, RNA polymerases, and RNA-binding proteins. NgAgo mainly binds to the transcripts inside genes or in their upstream sequences. Interestingly, the top enriched motif of peaks was the same as that of miR-1289, suggesting that NgAgo may regulate gene expression post-transcriptionally. GO enrichment analysis showed that the peak-associated genes were enriched in transmembrane transport processes. These results revealed that NgAgo binds RNA and may function in post-transcriptional regulation in vivo.

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Section: Discussionmentioning

confidence: 99%

Characterization of endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 cells

Jiang¹,

Lin²,

Pu³

et al. 2022

Biocell

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“…Cellular validation research has proved that tRFs alteration patterns can be served as CRC metastatic predictors. We conducted a systematic review of tRNA-Derived small RNAs involved in the pathogenesis of cancer types (20), Based on this, selected four tRFs that seem to be the role in cancer proliferation and metastasis. Moreover, we analyzed the MINTbase v2 database in order to check the pro ling of small RNA transcripts (supplementary data).…”

Section: Introductionmentioning

confidence: 99%

Tissue tRNA‐Derived Fragments (tRFs) as Potential Candidates Correlate with Invasiveness in Colorectal Carcinomas

Sahlolbei

Fattahi

Vafaei

et al. 2020

Preprint

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Abstract Background Colorectal cancer (CRC), which is one of lethal and invasive cancer with metastasis at the time of diagnosis. Despite, advanced therapy and surgical techniques, Patients with metastases CRC have a poor survival rate. Enhanced predictive biomarkers such as non-coding RNAs (ncRNAs) are needed at the time of diagnosis to better therapies. The tRNA- derived fragments (tRF) are non-protein-coding sequences that probably function as oncogenes or tumor suppressor genes, tRFs produced under physiological or stress conditions from enzymatically cleavage in tRNAs. Methods Documenting the tRNA-derived fragments (tRF) in colorectal cancer and showed that it is significantly down-regulated in colorectal tissue tumors compared to normal tissue samples. Since data distribution was not normal, Mann-Whitney, Kruskal-Wallis, and Spearman correlation coefficient tests were used for analysis. Results Among several tRF, quantitative real-time PCR analyses declared that tRF/miR-1280, tRNA-derived fragment-Asp, tRNA-derived fragment-Val down-regulated in CRC. Notably, its expression was correlated with invasive stage and metastases (p < 0.05). Conclusions This research can elucidate the association between tRFs with the invasion stage and CRC clinical pathology, our results not only expand better understanding about tRNA-derived fragments species in CRC but also highlight the potential usages of tRFs as a promising biomarker in the prognosis of metastatic cancer.

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Dynamic Signature of tRNA-Derived small RNAs in Cancer Pathogenesis as a Promising Valuable Approach

Cited by 2 publications

References 37 publications

Characterization of endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 cells

Characterization of endogenous nucleic acids that bind to NgAgo in Natronobacterium gregoryi sp2 cells

Tissue tRNA‐Derived Fragments (tRFs) as Potential Candidates Correlate with Invasiveness in Colorectal Carcinomas

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