Dynamic‐SIMS imaging and quantification of inorganic ions in frozen‐hydrated plant samples

Dérue, Cédric; Gibouin, David; Demarty, Mauríce; Verdus, Marie‐Claire; Lefebvre, F.; Thellier, Michel; Ripoll, Camille

doi:10.1002/jemt.20270

Cited by 31 publications

(17 citation statements)

References 20 publications

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“…/ 23 Na ? ratio of *10 in these cells is in general agreement to the ratios of these elements typically observed in animal and plant cells (Chandra et al 2002;Chandra 2005;Derue et al 2006).…”

Section: Resultssupporting

confidence: 74%

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Subcellular localization of silicon and germanium in grass root and leaf tissues by SIMS: evidence for differential and active transport

et al. 2010

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Silicon transport and incorporation into plant tissue is important to both plant physiological function and to the influence plants have on ecosystem silica cycling. However, the mechanisms controlling this transport have only begun to be explored. In this study, we used secondary ion mass spectrometry (SIMS) to image concentrations of Si in root and shoot tissues of annual blue grass (Poa annua L.) and orchard grass (Dactylis glomerata L.) with the goal of identifying control points in the plant silica uptake pathway. In addition, we used SIMS to describe the distributions of germanium (Ge); the element used to trace Si in biogeochemical studies. Within root tissue, Si and Ge were localized in the suberized thickwalled region of endodermal cells, i.e. the proximal side of endodermal cells which is in close association to the casparian strip. In leaves, Si was present in the cell walls, but Ge was barely detectable. The selective localization of Si and Ge in the proximal side of endodermal cell walls of roots suggests transport control is exerted upon Si and Ge by the plant. The absence of Si in most root cell walls and its presence in the cell walls of leaves (in areas outside of the transpiration terminus) suggests modifications in the chemical form of Si to a form that favors Si complexation in the cell walls of leaf tissue. The low abundance of Ge in leaf tissue is consistent with previous studies that suggest preferential transport of Si relative to Ge.

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Section: Resultssupporting

confidence: 74%

“…Linton and Goldsmith 1992;Lazof et al 1996;Grignon et al 1996;Derue et al 2006). The choice for a particular method of sample preparation is problem-and sample typedependent.…”

Section: Passive Vs Active Mechanisms Of Ge-si Fractionationmentioning

confidence: 99%

Subcellular localization of silicon and germanium in grass root and leaf tissues by SIMS: evidence for differential and active transport

et al. 2010

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“…Secondary ionization MS has been used for imaging studies of metal ions on plant surfaces (Derue et al, 2006;Gea et al, 1994;Gojon et al, 1996;Heard et al, 2002;Jauneau et al, 1994;Mangabeira et al, 2006). Recently, localization of phenolic compounds from wood stems has been examined by time-of-flight (TOF) SIMS (Imai et al, 2005;Saito et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Direct profiling and imaging of plant metabolites in intact tissues by using colloidal graphite‐assisted laser desorption ionization mass spectrometry

et al. 2008

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SummaryLaser desorption/ionization (LDI)-based imaging mass spectrometry (MS) has been applied to several biological systems to obtain information about both the identities of the major chemical species and their localization. Colloidal graphite-assisted LDI (GALDI) MS imaging was introduced for the imaging of small molecules such as phospholipids, cerebrosides, oligosaccharides, flavonoids, and other secondary metabolites with high spatial homogeneity due to finely dispersed particles. Mass profiles and images of Arabidopsis thaliana have been recorded directly from various plant surfaces and cross sections. The main targeted metabolites were flavonoids and cuticular waxes, both of which are important in many aspects of functional genomics, proteomics, and metabolomics. The mass spectral profiles revealed tissue-specific accumulation of flavonoids in flowers and petals. In addition, many other location-specific ions were observed. The location and the degree of light-induced accumulation of flavonoids in stem sections were successfully probed by GALDI MS.

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“…Use of secondary ion mass spectrometry (SIMS) for this purpose did not yet give fully satisfactory results (Dérue et al, 1999(Dérue et al, , 2006. SIMS requires very flat specimens and because of matrix effects (Benninghoven et al, 1987) it is not well suited to carry out absolute quantification, despite high sensitivity.…”

Section: Introductionmentioning

confidence: 96%

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

Tylko¹,

Mesjasz‐Przybyłowicz²,

Przybyłowicz³

2006

Microscopy Res & Technique

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Elemental microanalysis of biological material in the frozen-hydrated state using in-vacuum proton induced X-ray emission is described for the first time. For this purpose, a commercially available cryotransfer system was modified and coupled to the experimental chamber of the nuclear microprobe (NMP). The analyzed material was frozen in propane cooled by liquid nitrogen, fractured, carbon coated, and transferred onto the cold stage (100 K) of the nuclear microprobe chamber. Micro-PIXE and simultaneous proton backscattering was performed using a 3 MeV proton beam. Quantitative results were obtained by the standardless method, and tested using 20% gelatin standards. Monitoring of the gas composition inside the system by means of mass spectrometry performed before, during, and after proton bombardment showed good stability of the analyzed material for proton currents not exceeding 150 pA. Average concentrations of light elements (C, N, O, and indirectly H) were also obtained by the proton backscattering technique. No losses of elements measurable by particle-induced X-ray emission (PIXE) during proton irradiation were found during repetitive, short analyses of the same micro areas of gelatin standards. Measurements of thick sections of selected plant and animal material in the frozen-hydrated state-leaf sections of the plant Senecio anomalochrous Hilliard (Asteraceae) and larvae of Chysolina pardalina Fabricius (Chrysomelidae)-showed very good preservation of morphology and elemental distribution. Limits of detection of the order of a few micro g g(-1) were obtained for most elements.

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Dynamic‐SIMS imaging and quantification of inorganic ions in frozen‐hydrated plant samples

Cited by 31 publications

References 20 publications

Subcellular localization of silicon and germanium in grass root and leaf tissues by SIMS: evidence for differential and active transport

Subcellular localization of silicon and germanium in grass root and leaf tissues by SIMS: evidence for differential and active transport

Direct profiling and imaging of plant metabolites in intact tissues by using colloidal graphite‐assisted laser desorption ionization mass spectrometry

X‐ray microanalysis of biological material in the frozen‐hydrated state by PIXE

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