In interplanetary trajectory optimization, events such as planetary gravitational-assist maneuvers (swingbys) and deep-space maneuvers can be added/removed from the trajectory plan to reduce the cost or the flight time. This renders the number of design variables in the optimization problem variable. Global optimization methods that optimize this type of multimodal objective function can only handle problems with a fixed number of design variables. This paper presents the structured-chromosome evolutionary algorithm framework that is developed to handle variable-size design space optimization problems. In this framework, a solution (chromosome) is represented by a hierarchical data structure where the genes in the chromosome are classified as dependent and nondependent genes. This structure provides the capability to apply genetic operations between solutions of different lengths, and thus to automatically determine the number of swingbys, the planets to swingby, launch and arrival dates, and the number of deep-space maneuvers, as well as their locations, magnitudes, and directions, in an optimal sense. This new method is applied to several interplanetary trajectory design problems. Results show that solutions obtained using this tool match known solutions for some complex problems. A comparison between genetic algorithms and differential evolution in the structured-chromosome framework is presented. Nomenclature a = first selected chromosome a = thrust acceleration vector b = second selected chromosome c = third selected chromosome F = fitness (cost function) F 0 = modified fitness (cost function) f = flight direction G = derating function h = pericenter altitude, km h = normalized swingby pericenter altitude iter = iteration m = number of swingby maneuvers NR = number of runs n = number of deep-space maneuvers in a single leg R = mean radius, km r = heliocentric position vector in inertial frame, km r = spacecraft position vector r = spacecraft acceleration vector SC = success counter SR = success rate sol = solution T = time of flight, days t = Julian date tmp = temporary chromosome u = solution feature v ∞ = hyperbolic velocity vector relative to the planet, km∕s w = differential weight ΔV x = x component of impulsive maneuver velocity ΔV y = y component of impulsive maneuver velocity ΔV z = z component of impulsive maneuver velocity Δv = impulsive maneuver velocity vector, km∕s Δv T = total mission cost, km∕s ε = epoch of a deep-space maneuver as a fraction of transfer time η = swingby plane rotation angle, rad μ = gravitational constant, km 3 ∕s 2 Subscripts a = arrival d = departure l = leg number p = swingby's planet ps = powered swingby req = required s∕c = spacecraft T = total Superscripts − = incoming = outgoing