Dynamic state of mugineic acid and analogous phytosiderophores in Fe-deficient barley

Iron Nutrition of Peanut Enhanced by Mixed Cropping with Maize: Possible Role of Root Morphology and Rhizosphere Microflora

“…[2,14,15] Maize, on the other hand, is characterized as a ''Strategy II'' plant, and responds to Fe deficiency by synthesising and releasing phytosiderophores which are able to form very stable complexes with Fe(III) in the soil. [16][17][18][19] Our data indicate that in mixed culture maize not only acquired adequate Fe to meet its own demand, but also improved the Fe status of associated peanut. Most Fe deficiency occurs on high pH soils, especially on calcareous soils with marginal levels of available Fe.…”

Diurnal rhythm of release of phytosiderophores and uptake rate of zinc in iron-deficient wheat

“…The mobilized and reduced Fe was then complexed with ferrozine and the absorbance measured at 562 nm and calculated as Fe equivalents. For the identification of the phytosiderophores the concentrated root exudate was purified by ion exchange chromatography (Takagi et al 1984), and compared with various phytosiderophore standards (epi-3-hydroxy-mugineic acid, 2"-deoxy-mugineic acid, and mugineic acid) by the HPLC method of Mori et al (1987). More than 90% of the Fe-mobilizing capacity of the root exudates from Fe-deficient plants could be attributed to the phytosiderophores 2'-deoxy-mugineic acid.…”

Roots of Iron-Efficient Maize also Absorb Phytosiderophore-Chelated Zinc

“…Thereafter, phytosiderophores were partially purified by gradient elution through a Dowex 50 WX 8 cation-exchange column (Serva, Heidelberg, Germany) using 0.5 to 2.0 M HCl as eluant. In the purified samples, phytosiderophores were quantified by HPLC (Mori et al, 1987) and identified exclusively as DMA in wheat or as eHMA (>95%), mugineic acid, and DMA (<5%) in barley. ',C-labeled phytosiderophores were obtained from barley plants by the same procedure, except that during a period of 2 weeks Fe-deficient barley plants were placed in a plexiglass box and exposed to 4.4 MBq of 14C0,, produced by the reaction of Ba14C0, with 50% lactic acid.…”