Dynamic temporal and spatial regulation of the cdk inhibitor p57kip2 during embryo morphogenesis

Westbury, Josh; Watkins, Marie; Ferguson-Smith, Anne C.; Smith, Janet

doi:10.1016/s0925-4773(01)00512-3

Cited by 43 publications

(54 citation statements)

References 18 publications

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“…Our findings in the rat lung are consistent with the published data in the mouse 40 and hamster, 46 in that p57…”

Section: Rat P57supporting

confidence: 93%

“…This difference may be due to different methodologies used. In mouse, Kochilas et al 39 found an expression pattern that was similar to that reported here, whereas others 37,40 described expression restricted to the endocardial cells of mouse at comparative embryonic stages. This discrepancy can be attributed to observational differences.…”

Section: Rat P57supporting

confidence: 85%

“…[17][18][19] The high expression rates of p57 Kip2 demonstrated in skeletal muscle during early stages of rat development are similar to those reported in mouse. 37,40 However, here we demonstrate relatively high levels that persisted until birth. After birth, the levels of p57…”

Section: Rat P57contrasting

confidence: 44%

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p57Kip2 (cdkn1c): sequence, splice variants and unique temporal and spatial expression pattern in the rat pancreas

Potikha

Kassem

Haber

et al. 2005

Laboratory Investigation

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The cyclin-dependent kinase (CDK) inhibitor p57Kip2 (CDKN1C) is a negative regulator of cell proliferation, binding to a variety of cyclin-CDK complexes and inhibiting their kinase activities in vitro. The p57Kip2 gene is imprinted and the maternal allele is expressed in terminally differentiated cells, including human b-cells. Somatic loss of p57Kip2 expression is associated with increased b-cell proliferation in the focal form of Hyperinsulinism of Infancy. We cloned and sequenced the rat ortholog of p57 Kip2 , and demonstrate that it is highly homologous to the mouse gene. However, the human and rodent genes are quite divergent. Despite having highly homologous C-and N-terminal domains, the mid-portion of the human gene is entirely different from that of its rodent counterparts. Expression of p57Kip2 was evaluated during fetal and postnatal development, and a highly cell-specific, temporal and spatial expression profile was found. In contrast to other tissues, the expression pattern in rat pancreas was entirely opposite from that previously reported in man, with high levels of expression in rodent exocrine cells, but no expression in b-cells during any stage of development. These findings demonstrate that p57Kip2 expression is highly regulated. In the pancreas, the functional significance of this gene appears to be quite different in humans when compared with rodents, suggesting that a better understanding of the function of this protein may provide new insights into the mechanisms involved in the control of human b-cell mass.

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“…Our findings in the rat lung are consistent with the published data in the mouse 40 and hamster, 46 in that p57…”

Section: Rat P57supporting

confidence: 93%

Section: Rat P57supporting

confidence: 85%

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p57Kip2 (cdkn1c): sequence, splice variants and unique temporal and spatial expression pattern in the rat pancreas

Potikha

Kassem

Haber

et al. 2005

Laboratory Investigation

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“…Kip2 was not only expressed by terminally differentiated cells, but was present in cells undergoing shape-changing, mitosis, and cell division (Westbury et al, 2001). …”

Section: P57mentioning

confidence: 99%

Origin and characteristics of glycogen cells in the developing murine placenta

Coan

Conroy

Burton

et al. 2006

Developmental Dynamics

210

230

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The junctional zone (Jz) of the mouse placenta consists of two main trophoblast populations, spongiotrophoblasts and glycogen cells (GCs), but the development and function of both cell types are unknown. We conducted a quantitative analysis of GC size, number, and invasion of cells into the decidua across gestation. Furthermore, we identified markers of GC function to investigate their possible roles in the placenta. While the spongiotrophoblast cell volume doubles, and cell number increases steadily from E12.5 to E16.5, there is a remarkable 80-fold increase in GC numbers. This finding is followed by a notable decrease by E18.5. Surprisingly, the accumulation of GCs in the decidua did not fully account for the decrease in GC number in the Jz, suggesting loss of GCs from the placenta. Glucagons were detected on GCs, suggesting a steady glucose release throughout gestation. Connexin31 staining was shown to be specific for GCs. GC migration and invasion may be facilitated by temporally regulated expression of matrix metalloproteinase 9 and the imprinted gene product, Decorin.

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“…Immunostaining can be used to identify proliferating cells, using an antibody to Ki67 (1/1,000 dilution), to establish identity, using an antibody to Myf-5 (1/1,000 dilution), or to investigate gene expression (see Section 4.5). Immunostaining can be achieved using a number of methods, the following (described in (28,29)) is used routinely by the authors:…”

Section: Immunohistochemistrymentioning

confidence: 99%

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Merrick

Chen

Larner

et al. 2010

JoVE

Self Cite

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Cultured embryonic and adult skeletal muscle cells have a number of different uses. The micro-dissected explants technique described in this chapter is a robust and reliable method for isolating relatively large numbers of proliferative skeletal muscle cells from juvenile, adult or embryonic muscles as a source of skeletal muscle stem cells. The authors have used micro-dissected explant cultures to analyse the growth characteristics of skeletal muscle cells in wild-type and dystrophic muscles. Each of the components of tissue growth, namely cell survival, proliferation, senescence and differentiation can be analysed separately using the methods described here. The net effect of all components of growth can be established by means of measuring explant outgrowth rates. The micro-explant method can be used to establish primary cultures from a wide range of different muscle types and ages and, as described here, has been adapted by the authors to enable the isolation of embryonic skeletal muscle precursors.Uniquely, micro-explant cultures have been used to derive clonal (single cell origin) skeletal muscle stem cell (SMSc) lines which can be expanded and used for in vivo transplantation. In vivo transplanted SMSc behave as functional, tissue-specific, satellite cells which contribute to skeletal muscle fibre regeneration but which are also retained (in the satellite cell niche) as a small pool of undifferentiated stem cells which can be re-isolated into culture using the micro-explant method.

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Dynamic temporal and spatial regulation of the cdk inhibitor p57kip2 during embryo morphogenesis

Cited by 43 publications

References 18 publications

p57Kip2 (cdkn1c): sequence, splice variants and unique temporal and spatial expression pattern in the rat pancreas

p57Kip2 (cdkn1c): sequence, splice variants and unique temporal and spatial expression pattern in the rat pancreas

Origin and characteristics of glycogen cells in the developing murine placenta

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

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