Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis

Otal, Yavuz; Kahraman, Fatih; Haydar, Fadime Güllü; Erel, Özcan

doi:10.3906/sag-1904-55

Cited by 19 publications

(16 citation statements)

References 29 publications

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“…When we compared mild and severe/critical groups in our study, we found that total thiol, native thiol, and disul de levels decreased signi cantly (Table 3). This decrease in disul de levels in patients with severe pneumonia was thought to be a result of the conversion of proteins to advanced oxidation products [34]. While IMA levels, one of the indicators of oxidative stress in COVID-19 patients, were found to be high in some studies, no signi cant increase was found in others [13,22,33].…”

Section: Discussionmentioning

confidence: 98%

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

Tepebaşı

İlhan

Temal

et al. 2022

Preprint

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Background: COVID-19 disease, which spreads all over the world, is a disease characterized by widespread inflammation and affects many organs, especially the lungs. The resulting inflammation can lead to the generation of reactive oxygen radicals, leading to oxidative DNA damage.Methods and Results: Pneumonia severity of a total of 95 hospitalized patients with positive RT-PCR test was determined and divided into three groups mild, moderate, and severe/critical. Inflammation markers (Neutrophil-lymphocyte ratio, serum reactive protein, procalcitonin, etc.) were determined, and IL-10 and IF γ measurements were analyzed using the enzyme-linked immunosorbent assay method. In the evaluation of oxidative damage, total thiol, native thiol, disulfide, and IMA levels were determined by measuring spectrophotometrically. The percentage of tail DNA obtained by the comet assay method was used to determine oxidative DNA damage. As a result, when mild and severe/critical groups were compared, we found that total thiol, native thiol, and disulfide levels decreased significantly in the severe/critical group due to the increase in inflammation markers and cytokine levels (p <0.05). We could not detect any significance in IMA levels between the groups (p> 0.05). At the same time, we determined that there was an increase in the tail DNA percent level, that is, DNA damage, due to the increased oxidative effect.Conclusion: As a result, we found that increased inflammation and reactive oxygen species due to the increase in pneumonia increased in those with severe and severe pneumonia, which in turn damaged DNA. We think that it would be useful to follow up on the DNA damage in these patients in terms of neurodegenerative diseases and cancer development.

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Section: Discussionmentioning

confidence: 98%

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

Tepebaşı

İlhan

Temal

et al. 2022

Preprint

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“…Advanced oxidation in BS may show us the severity of the disease. Some studies have reported cases of advanced oxidation (24,25).…”

Section: Discussionmentioning

confidence: 99%

Intracellular and extracellular thiol/disulfide homeostasis in bacterial sepsis

Avcıoglu¹,

Otal²,

Erel³

2022

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Amaç: Bakteriyel sepsis (BS) patogenezinde birçok mediatör ve biyobelirteç rol oynamakta olup tam olarak aydınlatılamamıştır. Çalışmanın amacı oksidatif stresin BS patogenezine katkısını değerlendirmek için BS'de hücre içi okside glutatyon (GSSG)/indirgenmiş glutatyon (GSH) ve serum tiyol/disülfid (hücre dışı) homeostazis testlerini analiz etmektir. Gereç ve yöntem: Bu çalışmaya 49 hasta ve 43 sağlıklı gönüllü olmak üzere toplam 92 kişi dahil edildi. Sepsis ve septik şok için üçüncü uluslararası konsensüs tanımlarında tanımlanan BS kriterlerinin yüksek olasılık ve pozitif kan kültürü olan olgular çalışmaya dahil edildi. Kan örnekleri, hücre içi ve hücre dışı tiyol/disülfid homeostazis testleri için kullanıldı. Bulgular: BS ve kontrol grubu karşılaştırıldığında, BS grubunda GSH, total glutatyon (GSH+GSSG), disülfid, İndeks-3, nativ tiyol ve toplam tiyol düzeyleri kontrol grubuna göre anlamlı derecede düşüktü (İndeks-3 için p<0,001 ve disülfid için p<0.05). GSSG düzeyleri açısından iki grup arasında anlamlı fark saptanmadı. BS grubunda yaş, CRP, PCT, WBC ve RDW, MCV, N/L ve Index-2 düzeyleri kontrol grubuna göre anlamlı derecede yüksekti (p<0.05). Sonuçlar: BS'de total tiyol ve nativ tiyol düzeyleri ile GSH ve total glutatyon düzeyleri azaldı. Bozulmuş hücre içi ve hücre dışı tiyol/disülfid homeostazisinin oksidatif stresi artırarak BS patogenezi üzerinde etkileri olabilir

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“…Disulfide structures (S-S), which are formed as a result of OS are reduced to thiol groups again, and thiol balance is achieved 12. In many studies, the measurement of dynamic thiol disulfide balance has been shown as an indicator of increased free radical levels in various diseases 13–16. The increase in OS causes free radical damage at the N-terminal end of albumin molecules and decreases the binding capacity of albumin.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia

Erdal

Çiftçiler

Tuncer

et al. 2023

Journal of Investigative Medicine

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This is the first study to evaluate both the dynamic thiol-disulfide homeostasis and ischemia-modified albumin (IMA) levels in patients with chronic lymphocytic leukemia (CLL). Twenty-nine patients with CLL and 20 controls were included in the study. The dynamic thiol-disulfide balance was determined by the newly developed colorimetric method by Erel. IMA levels were determined by the cobalt binding test. We found that total antioxidant status levels were lower while total oxidant status (TOS) and oxidative stress index (OSI) levels were significantly higher in patients with CLL than controls. Moreover, native and total thiol levels were found to be statistically significant between the study and control groups (p<0.001), whereas no statistically significant difference was noted for IMA levels (p=0.365). A negative correlation was observed between native and total thiol levels, leukocyte, lymphocyte, and TOS. Total bilirubin showed positive correlation with direct bilirubin and alkaline phosphatase. In addition, IMA levels showed a positive correlation with OSI. This study highlights measurement of native and total thiol and IMA levels in patients with CLL for the first time. Dynamic thiol-disulfide homeostasis may contribute in the pathophysiological mechanism, and follow-up to disease in patients with CLL.

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Dynamic thiol/disulfide homeostasis as oxidative stress marker in diabetic ketoacidosis

Cited by 19 publications

References 29 publications

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

The effect of inflammation and oxidative stress on DNA damage in COVID-19 patients

Intracellular and extracellular thiol/disulfide homeostasis in bacterial sepsis

Evaluation of dynamic thiol-disulfide homeostasis and ischemia-modified albumin levels in patients with chronic lymphocytic leukemia

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