Dynamic tracking of p21 mRNA in living cells by sticky-flares for the visual evaluation of the tumor treatment effect

Zhao, Tingting; Dong, Fengqi; Hu, Xinlong; Xu, Yanli; Wei, Wen-mei; Li, Rui; Fang, Yu; Fang, Weijun; Shen, Yujun; Zhang, Zhongping

doi:10.1039/d1nr05418j

Cited by 6 publications

(4 citation statements)

References 50 publications

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“…The detection sensitivity of this work was comparable to that of other AuNPs and other nanomaterial-based assays (Table S2). ,,,− The kinetic study suggested that the polyA 20 -mediated sticky flares reached equilibrium in solution within 1.5 h (Figure S3). We then investigated the specificity of polyA 20 -mediated sticky flares by incubating probes with other kinds of DNA targets (perfectly matched DNA targets, the corresponding single-base mismatched DNA targets (SM-target), and nonspecific survivin DNA corresponding to the partial sequence of survivin mRNA).…”

Section: Resultsmentioning

confidence: 99%

“…Fluorescent spherical nucleic acid (SNA) is a typical nanoprobe for intracellular molecular imaging, which is always composed of AuNPs and fluorescent dye-labeled DNA probes. It has attracted particular attention due to its high uptake efficiency, low background signal, easy modification, and good biocompatibility. − However, most approaches based on SNA suffer from shortcomings including limited detection sensitivity, long detection time, and high detection cost. The main reason for this phenomenon is that the interface effect of AuNPs affects the activity of DNA reaction, especially DNA hybridization and replacement. − Due to the interaction between DNA bases and nanomaterials, the conformation of DNA on the nanointerface is often distorted, which greatly affects the hybridization rate and efficiency of DNA. , Recent research about DNA hybridization on SNAs by Mirkin et al suggested that DNA hybridization heavily depended on the charge repulsion, surface density, and strand conformation on surface. , Our group has systematically investigated the DNA reaction on the interface of AuNPs and found that the controllable assembly of DNA with defined density and space was crucial to improve the efficiency and kinetics of DNA hybridization, which was hard to achieve through traditional thio-DNA-mediated DNA–AuNPs assembly .…”

Section: Introductionmentioning

confidence: 99%

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Spatiotemporal Monitoring of Subcellular mRNAs In Situ via Polyadenine-Mediated Dual-Color Sticky Flares

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

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Spatiotemporal monitoring of multiple low-abundance messenger RNAs (mRNAs) is vitally important for the diagnosis and pathologic analysis of cancer. However, it remains a clinical challenge to monitor and track multiple mRNAs location simultaneously in situ at subcellular level with high efficiency. Herein, we proposed polyA-mediated dual-color sticky flares for simultaneous imaging of two kinds of intracellular mRNA biomarkers. Two kinds of fluorescent DNA specific for GalNac-T mRNA and c-Myc mRNA were functionalized onto gold nanoparticles (AuNPs) through efficient polyadenine (polyA) attachment. By tuning polyA length, the lateral spacing and densities of DNA on AuNPs could be precisely engineered. Compared to the traditional thio-DNA-modified nanoprobes, the uniformity, detection sensitivity, and response kinetics of sticky flares were greatly improved, which enables live-cell imaging of mRNAs with enhanced efficiency. With a sticky-end design, the fluorescent DNA could dynamically trace mRNAs after binding with target mRNAs, which realized spatiotemporal monitoring of subcellular mRNAs in situ. Compared to one target mRNA imaging mode, the multiple target imaging mode allows more accurate diagnosis of cancer. Furthermore, the proposed polyA-mediated dual-color sticky flares exhibit excellent cell entry efficiency and low cytotoxicity with a low-cost and simple assembling process, which provide a pivotal tool for multiple targets imaging in living cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spatiotemporal Monitoring of Subcellular mRNAs In Situ via Polyadenine-Mediated Dual-Color Sticky Flares

Zhu

et al. 2023

ACS Appl. Mater. Interfaces

View full text Add to dashboard Cite

show abstract

“…When the target is present, the Cy5 recognition sequence and the target form a more stable double strand and fall off the AuNP, forming a specific in situ detection. Our group also designed different stickyFlares to detect telomerase RNA (hTR) and tumor suppressor gene p21 mRNA in cells, respectively ( Figure 4B ) ( Wu et al, 2018 ; Zhao et al, 2021 ). The main difference between stickyFlares and nanoFlare is whether it can determine and track the spatial distribution of RNA in cells.…”

Section: Dna-based Fluorescent Probesmentioning

confidence: 99%

Section: Dna-based Fluorescent Probesmentioning

confidence: 99%

DNA-enabled fluorescent-based nanosensors monitoring tumor-related RNA toward advanced cancer diagnosis: A review

Dong

Yan

Dong

et al. 2022

Front. Bioeng. Biotechnol.

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As a burgeoning non-invasive indicator for reproducible cancer diagnosis, tumor-related biomarkers have a wide range of applications in early cancer screening, efficacy monitoring, and prognosis predicting. Accurate and efficient biomarker determination, therefore, is of great importance to prevent cancer progression at an early stage, thus reducing the disease burden on the entire population, and facilitating advanced therapies for cancer. During the last few years, various DNA structure-based fluorescent probes have established a versatile platform for biological measurements, due to their inherent biocompatibility, excellent capacity to recognize nucleic and non-nucleic acid targets, obvious accessibility to synthesis as well as chemical modification, and the ease of interfacing with signal amplification protocols. After decades of research, DNA fluorescent probe technology for detecting tumor-related mRNAs has gradually grown to maturity, especially the advent of fluorescent nanoprobes has taken the process to a new level. Here, a systematic introduction to recent trends and advances focusing on various nanomaterials-related DNA fluorescent probes and the physicochemical properties of various involved nanomaterials (such as AuNP, GO, MnO2, SiO2, AuNR, etc.) are also presented in detail. Further, the strengths and weaknesses of existing probes were described and their progress in the detection of tumor-related mRNAs was illustrated. Also, the salient challenges were discussed later, with a few potential solutions.

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SERS-fluorescence dual-mode nanoprobe for the detection and imaging of Bax mRNA during apoptosis

Lin

et al. 2023

Microchim Acta

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Dynamic tracking of p21 mRNA in living cells by sticky-flares for the visual evaluation of the tumor treatment effect

Abstract: Monitoring the expression level of intracellular tumor suppressor gene of p21 mRNA is essential to reveal the progress and prognosis of tumor. Methods widely reported for the detection of p21...

Cited by 6 publications

References 50 publications

Spatiotemporal Monitoring of Subcellular mRNAs In Situ via Polyadenine-Mediated Dual-Color Sticky Flares

Spatiotemporal Monitoring of Subcellular mRNAs In Situ via Polyadenine-Mediated Dual-Color Sticky Flares

DNA-enabled fluorescent-based nanosensors monitoring tumor-related RNA toward advanced cancer diagnosis: A review

SERS-fluorescence dual-mode nanoprobe for the detection and imaging of Bax mRNA during apoptosis

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