Urban road networks may benefit from left turn prohibition at signalized intersections regarding capacity, for particular traffic demand patterns. The objective of this paper is to propose a method for minimizing the total travel time by prohibiting left turns at intersections. With the flows obtained from the stochastic user equilibrium model, we were able to derive the stage generation, stage sequence, cycle length, and the green durations using a stage-based method which can handle the case that stages are sharing movements. The final output is a list of the prohibited left turns in the network and a new signal timing plan for every intersection. The optimal list of prohibited left turns was found using a genetic algorithm, and a combination of several algorithms was employed for the signal timing plan. The results show that left turn prohibition may lead to travel time reduction. Therefore, when designing a signal timing plan, left turn prohibition should be considered on a par with other left turn treatment options.