Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Zhang, Linpei; Li, Yuqiu; Meng, Wenqi; Ni, Yanying; Gao, Youhe

doi:10.1101/481697

Cited by 9 publications

(10 citation statements)

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“…We also compared the urinary differential proteins in the CUMS model to those in 15 other models, including a clinical model of autism [62] and a variety of animal models of Alzheimer's disease [11], Parkinson's disease [12], myocarditis [63] , chronic pancreatitis [64], unilateral ureteral obstruction model [65], astrocytoma [66], liver fibrosis [67] , pulmonary fibrosis [68] , glomerulosclerosis [69] , a model involving the injection of 10 tumor cells [70], the Walker-256 intracerebral tumor model [71], the Walker-256 liver tumor model [72], the Walker-256 subcutaneous model [73] and the Walker-256 lung metastasis model [74]. The comparison results are presented in Table S6.…”

Section: Discussionmentioning

confidence: 99%

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Huan

Wei

et al. 2020

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Background. Major depressive disorder (MDD) is a prevalent complex psychiatric disorder with a high prevalence rate. Because MDD is a systemic multifactorial disorder involving complex interactions and disturbances of various molecular pathways, there are no effective biomarkers for clinical diagnosis. Urine is not subjected to homeostatic control, allowing it to reflect the sensitive and comprehensive changes that occur in various diseases. In this study, we examined the urine proteome changes in a CUMS mouse model of MDD. Methods. Male C57BL/6 mice were subjected to chronic unpredictable mild stress for 5 weeks. The tail suspension test (TST) and sucrose consumption test (SCT) were then applied to evaluate depression-like behaviors. The urine proteomes on day 0 and day 36 in the CUMS group were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Results. A total of 45 differential proteins were identified, 24 of which have been associated with the pathogenic mechanisms of MDD, while 10 proteins have been previously suggested as MDD biomarkers. There was an average of two differential proteins that were identified through 1048574 random combination statistical analyses, indicating that at least 95% of the differential proteins were reliable and not the result of random combination. The differential proteins were mainly associated with blood coagulation, inflammatory responses and central nervous system development. Conclusions. Our preliminary results indicated that the urine proteome can reflect changes associated with MDD in the CUMS model, which provides potential clues for the diagnosis of clinical MDD patients.

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Section: Discussionmentioning

confidence: 99%

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Huan

Wei

et al. 2020

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“…Protein AMBP, a liver-specific precursor, is also a precursor of the heme-binding protein that counteracts the disruption of free haemoglobin (Van den Figure 4 The overlapping differential proteins in urine samples of the four different W256 tumour models. The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018). The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018).…”

Section: Comparison Of Urinary Proteins In Different Tumour Modelsmentioning

confidence: 99%

“…The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018). The comparison data is from previously published studies (Wu, Guo & Gao, 2017;Wei et al, 2018;Zhang et al, 2018). Full-size DOI: 10.7717/peerj.8462/ fig-5 Berg et al, 2017).…”

Section: Comparison Of Urinary Proteins In Different Tumour Modelsmentioning

confidence: 99%

Early changes in the urine proteome in a rat liver tumour model

Zhang

Gao

2020

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Background Urine, as a potential biomarker source among body fluids, can accumulate many early changes in the body due to the lack of mechanisms to maintain a homeostatic state. This study aims to detect early changes in the urinary proteome in a rat liver tumour model. Methods The tumour model was established with the Walker-256 carcinosarcoma cell line (W256). Urinary proteins at days 3, 5, 7 and 11 were profiled by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). Compared with controls, differential proteins were selected. Associations of differential proteins with cancer were retrieved. Results At days 3, 5, 7 and 11, five, fifteen, eleven and twelve differential proteins were identified, respectively. Some of the differential proteins were reported to be associated with liver cancer. This differential urinary protein pattern was different from the patterns in W256 subcutaneous, lung metastasis and intracerebral tumour models. Conclusions This study demonstrates that (1) early changes in urinary proteins can be found in the rat liver tumour model; (2) urinary proteins can be used to differentiate the same tumour cells grown in different organs.

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“…7). at early stages based on the data obtained from the studies of our laboratory published before [11,111,112] .…”

Section: Functional Analysis Of Differential Proteinsmentioning

confidence: 99%

Early changes in the urine proteome in a rat liver tumor model

Zhang

Gao

2019

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Urine, as a potential biomarker source among the body fluids, can accumulate many changes in the body due to the lack of a mechanism to maintain a homeostatic state. Previous studies have demonstrated that proteomic technology can find many potential biomarkers to reflect different diseases in the urine.This study aims to detect early changes in the urinary proteome in a rat liver tumor model. The tumor model was established with the Walker-256 carcinosarcoma cell line (W256). Compared to before the injection, ninety-five differential proteins were significantly changed in the experimental rats. At day 3, twelve proteins were identified in the absence of pathological changes, and four of them were altered at all four time-points (B2MG, VCAM1, HA11, and LG3BP). Seven had previously been associated with liver cancer. At day 5, fifty-two differential proteins were identified. At day 7 and day 11, there was a significant decrease in the body weight of the rats, and tumor tissue was observed in the liver. Fifty-two and forty differential proteins were changed significantly at day 7 and day 11, respectively. Of the proteins that were identified at these three time-points, and twenty-four were reported to be associated with liver cancer. Comparing the differential urinary proteins and biological processes of liver tumor model with those in different models of W256 grown in other organs, specific differential protein patterns were found among the four models, which indicates that the differential urinary proteins can reflect the differences when the same tumor cell grown in different organs.Significance: This study demonstrated that (1) the rat liver tumor model caused early changes in urinary proteins may give new insight into the early diagnosis of liver cancer; (2) the same tumor cell grown in different organs can be reflected in differential urinary proteins.All rats were housed under a standard 12-h light/12-h dark cycle, and the room temperature and humidity were maintained at a standard level (22 ± 1 °C, 65-70%). Tumor cell line and CultureWalker 256 tumor cells were cultured in the ascitic fluid of Wistar rats. All cells were harvested from the rats who were given an intraperitoneal injection of 107 Walker 256 carcinoma cells after two cycles of 7 d cell passage. Then, W256 cells were resuspended in phosphate-buffered saline (PBS) before injection. The viability of the cells was detected by the Trypan blue exclusion test using a Neubauer chamber. Rat models of liver cancerA tumor-bearing animal model was established in this study. All Wistar rats were randomly divided into different groups: the control group (n =5), and the Walker-256 (W256) tumor-bearing group (n =12).After anesthesia, the left medial lobe of the liver was exposed. W256 cells (2.5× 10 5 ) were visually injected under the hepatic capsule into this lobe. The injection volume is 0.1 ml. An equal volume of PBS was also injected into the same location of the control rats. Histological analysisIn the W256 model, the livers of the experimental and cont...

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Dynamic urinary proteomic analysis in a Walker 256 intracerebral tumor model

Cited by 9 publications

References 51 publications

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Urine proteome changes in a chronic unpredictable mild stress (CUMS) mouse model of major depressive disorder

Early changes in the urine proteome in a rat liver tumour model

Early changes in the urine proteome in a rat liver tumor model

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