Dynamic viscoelastic coagulometry of blood obtained from healthy chickens

Abstract

OBJECTIVE To assess feasibility of the use of a dynamic viscoelastic coagulometer on chicken blood and compare coagulation variables for fresh whole blood and sodium citrate–preserved whole blood as well as effects of 3 coagulation activators on blood from chickens. SAMPLE Blood samples from 30 hens. PROCEDURES Chickens were allowed to rest undisturbed for 1 hour. A blood sample was collected from an ulnar vein; 1.4 mL was analyzed immediately, and 1.8 mL was mixed with sodium citrate and subsequently recalcified and analyzed. A separate coagulation activator (glass beads, kaolin clay, or tissue factor) was in each of the 2 channels of the analyzer. Chickens were allowed a 1-hour rest period, and another blood sample was collected from the contralateral ulnar vein; it was processed in the same manner as for the first sample, except both channels of the analyzer contained the same coagulation activator. RESULTS Compared with fresh samples, citrated samples had higher values for activated clotting time and platelet function and lower clotting rates. Intra-assay coefficients of variation of coagulation profiles for citrated samples were markedly greater than the limit of 10%, whereas values for fresh samples were close to or < 10%. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that use of a dynamic viscoelastic coagulometer on chicken blood was feasible and that analysis of fresh whole blood from healthy chickens provided results with less variability than did analysis of citrated blood. Samples preserved with sodium citrate were associated with significant relative hypocoagulability, compared with results for fresh blood.

Determination of Coagulation Parameters by Whole Blood Dynamic Viscoelastic Coagulometry in Strigiformes

“…Intraassay coefficient of variation (CV) was determined in healthy chickens with fresh whole blood for ACT (6.85%) and CR (11.3%). 1 When the index of individuality was ≤1.7, reference intervals were calculated for the corresponding parameter.…”

“…5 Moreover, plasma-based tests such as PT and activated partial thromboplastin time do not integrate cellular components of coagulation and provide no information about the clot rate formation, the strength of the clot, or its dissolution. 1,7–9…”

“…Viscoelastography, such as dynamic viscoelastic coagulometry (DVC), can be used in real time to measure the viscoelastic properties of the blood clot starting from the onset of its formation to its lysis, and can be used to determine the coagulation status of a given patient: normal, hypocoagulable, or hypercoagulable. 1,7–9 Dynamic viscoelastic coagulometry is currently used in human medicine to help manage massive bleeding, assess a patient's condition during cardiopulmonary bypass and liver transplantation surgery, and regulate heparin therapy. 9–11 In veterinary medicine, reference intervals for DVC have been previously established in healthy horses and dogs, and whole blood analysis using DVC is also feasible in healthy chickens.…”

“…9–11 In veterinary medicine, reference intervals for DVC have been previously established in healthy horses and dogs, and whole blood analysis using DVC is also feasible in healthy chickens. 1,7,8…”

Evaluation of Recombinant Herpesvirus of Turkey Laryngotracheitis (rHVT-LT) Vaccine against Genotype VI Canadian Wild-Type Infectious Laryngotracheitis Virus (ILTV) Infection

“…The samples were stored at −80 • C until processed. At 5 and 12 dpi, 1.5 mL of blood was collected from the jugular vein with syringes previously filled with sodium citrate [41]. The samples were stored on ice until processed for PBMC isolation.…”