In this study, the effect of rotatory inertia on the transverse motion of uniform Rayleigh beam resting on Pasternak foundation subjected to harmonic magnitude moving load is investigated. We employed Fourier sine transform, Laplace integral transformation and convolution theorem as solution technique. It was observed from the results that, the amplitude of the deflection profile of the beam decreases with increase in the value of rotatory inertia and load natural frequency. Also increases in the values of the other structural parameters like shear modulus, foundation modulus, axial force, and damping coefficient lead to decreases in the deflection profile of the beam. It was also observed that the effect of the load natural frequency is more noticeable than that of the rotatory inertia.