The Lotka-Volterra predator-prey system x 0 ¼ x 2 xy, y 0 ¼ 2y þ xy is a good differential equation system for testing numerical methods. This model gives rise to mutually periodic solutions surrounding the positive fixed point (1,1), provided the initial conditions are positive. Standard finite-difference methods produce solutions that spiral into or out of the positive fixed point. Previously, the author [Roeger, J. Diff. Equ. Appl. 12 (9) (2006), pp. 937-948], generalized three different classes of nonstandard finite-difference methods that when applied to the predator-prey system produced periodic solutions. These methods preserve weighted area; they are symplectic with respect to a noncanonical structure and have the property that the computed points do not spiral. In this paper, we use a different approach. We apply the Jacobian matrix procedure to find a fourth class of nonstandard finite-difference methods. The Jacobian matrix method gives more general nonstandard methods that also produce periodic solutions for the predator-prey model. These methods also preserve the positivity property of the solutions.