Dynamics and conformational propensities of staphylococcal CntA

Abideen, Zain Ul; Ahmad, Ashfaq; Usman, Muhammad; Majaz, Sidra; Ali, Waqar; Noreen, Shumaila; Mahmood, Tariq; Nouroz, Faisal

doi:10.1080/07391102.2020.1782263

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…CntA prefers fluctuating the non-conserved regions rather than conserved where domain II turned out to be rigid and maintains a stable fold. Such findings are important to the researcher in field of drug-designing [ 93 ].…”

Section: Metallophores Produced By Staphylococcus Aureusmentioning

confidence: 99%

The Key Element Role of Metallophores in the Pathogenicity and Virulence of Staphylococcus aureus: A Review

Ghssein

Ezzeddine

2022

Biology

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The ubiquitous bacterium Staphylococcus aureus causes many diseases that sometimes can be fatal due to its high pathogenicity. The latter is caused by the ability of this pathogen to secrete secondary metabolites, enabling it to colonize inside the host causing infection through various processes. Metallophores are secondary metabolites that enable bacteria to sequester metal ions from the surrounding environment since the availability of metal ions is crucial for bacterial metabolism and virulence. The uptake of iron and other metal ions such as nickel and zinc is one of these essential mechanisms that gives this germ its virulence properties and allow it to overcome the host immune system. Additionally, extensive interactions occur between this pathogen and other bacteria as they compete for resources. Staphylococcus aureus has high-affinity metal import pathways including metal ions acquisition, recruitment and metal–chelate complex import. These characteristics give this bacterium the ability to intake metallophores synthesized by other bacteria, thus enabling it to compete with other microorganisms for the limited nutrients. In scarce host conditions, free metal ions are extremely low because they are confined to storage and metabolic molecules, so metal ions are sequestered by metallophores produced by this bacterium. Both siderophores (iron chelating molecules) and staphylopine (wide- spectrum metallophore) are secreted by Staphylococcus aureus giving it infectious properties. The genetic regulation of the synthesis and export together with the import of metal loaded metallophores are well established and are all covered in this review.

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Section: Metallophores Produced By Staphylococcus Aureusmentioning

confidence: 99%

The Key Element Role of Metallophores in the Pathogenicity and Virulence of Staphylococcus aureus: A Review

Ghssein

Ezzeddine

2022

Biology

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Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach

de Oliveira Silva,

Meneguello,

Formagio

et al. 2024

Photochem & Photobiology

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Staphylococcus aureus is a global challenge to the clinical field and food industry. Therefore, the development of antimicrobial photodynamic therapy (aPDT) has become one of the valuable methods to control this pathogen. The antibacterial activity of photoinactivation by erythrosine (Ery) against S. aureus has been reported, but its modes of action are unclear. This study aimed to employ a proteomic approach to analyze modes of action of Ery‐aPDT against S. aureus. We determined the antibacterial effect by Ery‐aPDT assays, quantified reactive oxygen species (ROS) and injury to the cell membrane, and determined protein expression using a proteomic approach combined with bioinformatic tools. Ery‐aPDT was effective in reducing S. aureus to undetectable levels. In addition, the increment of ROS accompanied the increase in the reduction of cell viability, and damage to cellular membranes was shown by sublethal injury. In proteomic analysis, we found 17 differentially expressed proteins. These proteins revealed changes mainly associated with defense to oxidative stress, energy metabolism, translation, and protein biosynthesis. Thus, these results suggest that the effectiveness of Ery‐aPDT is due to multi‐targets in the bacterial cell that cause the death of S. aureus.

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Dynamics and conformational propensities of staphylococcal CntA

Cited by 2 publications

References 55 publications

The Key Element Role of Metallophores in the Pathogenicity and Virulence of Staphylococcus aureus: A Review

The Key Element Role of Metallophores in the Pathogenicity and Virulence of Staphylococcus aureus: A Review

Multi‐targets of antimicrobial photodynamic therapy mediated by erythrosine against Staphylococcus aureus identified by proteomic approach

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