Dynamics and Determinants of HPV Infection: The Michigan HPV and Oropharyngeal Cancer (M-HOC) Study

Abstract

IntroductionHuman papillomavirus (HPV) is the primary cause of cervical and other anogenital cancers and is also associated with head and neck cancers. Incidence of HPV-related oropharyngeal squamous cell cancers (OPSCCs) is increasing, and HPV-related OPSCCs have surpassed cervical cancer as the most common HPV-related cancer in the USA. Given the multisite nature of HPV, there is strong interest in collecting data from both genital and oral sites, as well as associated data on social and sexual behaviours. The overarching goal of this study is to evaluate patterns of oral HPV infection incidence, clearance and persistence and their relationship to sexual behaviour history.Methods and analysisParticipants are recruited from two populations: college students at a large public university and general population from the surrounding area. At the first study visit, participants complete a detailed sexual history, health and behaviour questionnaire. Follow-up visits occur every 3–4 months over 3 years, when participants complete an abbreviated questionnaire. All participants provide a saliva sample at each visit, and eligible participants may provide a cervicovaginal self-swab. Genetic material isolated from specimens is tested for 15 high-risk and 3 low-risk HPV types. Statistical analyses will examine outcome variables including HPV prevalence, incidence, persistence and clearance. Logistic regression models will be used to estimate odds ratios and 95% confidence intervals for associations between the outcomes of interest and demographic/behavioural variables collected in the questionnaires. The longitudinal HPV infection data and detailed sexual history data collected in the questionnaires will allow us to develop individual-based network models of HPV transmission and will be used to parameterise multiscale models of HPV-related OPSC carcinogenesis.Ethics and disseminationThis study has been approved by the University of Michigan Institutional Review Board. All participants are consented in person by trained study staff. Study results will be disseminated through peer-reviewed publications.

DNA concentration from self samples for HPV testing

“…In an ongoing longitudinal study of oral and cervical HPV prevalence at the University of Michigan, using the HerSwab self-collection kit and covering women from ages 18 to 70, we find no such age relationship with DNA concentration. 2 This result appears consistent regardless of whether we control for time between collection and preservation in PreservCyt media, the time from preservation to extraction, and HPV result (Invalid vs. Positive/Negative).…”

HPV vaccination has not increased sexual activity or accelerated sexual debut in a college-aged cohort of men and women

“…Participants with a history of head and neck cancer were not eligible. Additional information is available in our study protocol [28]. Enrollment has been completed.…”

3D Oral and Cervical Tissue Models for Studying Papillomavirus Host‐Pathogen Interactions

“…HPV is responsible for virtually all cervical cancers. In addition, the incidence of HPV‐associated oral cancers has dramatically increased in recent years (Chaturvedi et al., 2011; Marur, D'Souza, Westra, & Forastiere, 2010), making oropharyngeal squamous cell carcinoma (OPSCC) the most common HPV‐related malignancy in the United States (Eisenberg et al., 2018). Of note, most HPV‐positive OPSCCs arise in the tonsil crypts.…”