2017
DOI: 10.1002/1873-3468.12844
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Dynamics and function ofERMproteins during cytokinesis in human cells

Abstract: The molecular mechanism that governs cytoskeleton-membrane interaction during animal cytokinesis remains elusive. Here, we investigated the dynamics and functions of ERM (Ezrin/Radixin/Moesin) proteins during cytokinesis in human cultured cells. We found that ezrin is recruited to the cleavage furrow through its membrane-associated domain in a cholesterol-dependent but largely Rho-independent manner. While ERMs are dispensable for furrow ingression, they play a pivotal role in contractile activity of the polar… Show more

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Cited by 19 publications
(16 citation statements)
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“…Other proteins may also participate as membrane anchors linking actomyosin to the plasma membrane independently of anilloseptin. Candidates include ezrin-radixin-moesin (ERM) family proteins that bind both F-actin and PIP2 and maintain cortical stability during mitosis and cytokinesis (Carreno et al, 2008;Roubinet et al, 2011;Kunda et al, 2012;Hiruma et al, 2017). Interestingly, Anillin depletion in human cells has been shown to enhance ERM localization to the furrow, although ERMs did not track well with the resulting oscillating furrows (Hiruma et al, 2017).…”
Section: Composition Of Membrane Microdomains and Protein Anchorsmentioning
confidence: 99%
See 1 more Smart Citation
“…Other proteins may also participate as membrane anchors linking actomyosin to the plasma membrane independently of anilloseptin. Candidates include ezrin-radixin-moesin (ERM) family proteins that bind both F-actin and PIP2 and maintain cortical stability during mitosis and cytokinesis (Carreno et al, 2008;Roubinet et al, 2011;Kunda et al, 2012;Hiruma et al, 2017). Interestingly, Anillin depletion in human cells has been shown to enhance ERM localization to the furrow, although ERMs did not track well with the resulting oscillating furrows (Hiruma et al, 2017).…”
Section: Composition Of Membrane Microdomains and Protein Anchorsmentioning
confidence: 99%
“…Candidates include ezrin-radixin-moesin (ERM) family proteins that bind both F-actin and PIP2 and maintain cortical stability during mitosis and cytokinesis (Carreno et al, 2008;Roubinet et al, 2011;Kunda et al, 2012;Hiruma et al, 2017). Interestingly, Anillin depletion in human cells has been shown to enhance ERM localization to the furrow, although ERMs did not track well with the resulting oscillating furrows (Hiruma et al, 2017). Supervillin, which can bind F-actin, myosin and the plasma membrane, is another candidate auxilliary anchor protein that acts during cytokinesis (Pestonjamasp et al, 1995(Pestonjamasp et al, , 1997Fang et al, 2010;Smith et al, 2010Smith et al, , 2013Hiruma et al, 2017).…”
Section: Composition Of Membrane Microdomains and Protein Anchorsmentioning
confidence: 99%
“…Line profile analysis was referred to the previous study [23]. Dividing cells with a furrow width less than 10 µm were selected for line profile analysis along the cortex of fixed cells.…”
Section: Line Profile Analysismentioning
confidence: 99%
“…mRNAs were synthesized in vitro with linearized template plasmids using a RiboMax Large Scale RNA Production System-T7 (Promega) supplemented with Ribo m 7 G Cap Analog (Promega) in reaction mixtures as described previously 40 . Template plasmids for EGFP-Anillin-Cterminal were constructed using cDNA from a pEGFP-Anillin-Cterminal vector 41 . Template plasmids for EGFP-PRC1 were constructed using human full-length PRC1 cDNA PCR amplified from the HeLa cDNA library.…”
Section: In Vitro Fertilization (Ivf)mentioning
confidence: 99%