Dynamics of ammonia-oxidizing archaea and bacteria populations and contributions to soil nitrification potentials

Taylor, Alfred; Zeglin, Lydia H.; Wanzek, Thomas; Myrold, David D.; Bottomley, Peter J.

doi:10.1038/ismej.2012.51

Cited by 164 publications

(95 citation statements)

References 29 publications

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“…This trend is clearly noticeable in QY soils, which is consistent with the previous observations of AOA dominance in a range of soils (He et al 2007;Taylor et al 2012). Moreover, the higher ratio of AOA to AOB in acid QY soils as compared to other non-acidic soils further confirmed the dominant role of AOA in acid soil habitats (He et al 2007).…”

Section: Discussionsupporting

confidence: 91%

Contrasting response of nitrification capacity in three agricultural soils to N addition during short-term incubation

Shen

Zhang

2014

J Soils Sediments

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Purpose Human disturbance is a major culprit driving imbalances in the biological transformation of nitrogen from the nonreactive to the reactive pool and is therefore one of the greatest concerns for nitrogen (N) cycling. The objective of this study was to compare potential nitrification rates and the abundance of ammonia oxidizers responsible for nitrification, with the amendment of external N in different agricultural soils. The abundance of ammonia oxidizing bacteria (AOB) and archaea (AOA) at the end of incubation in the soil microcosms was determined using the real-time PCR.Results and discussion There was a significant decrease in ammonium concentration in the QY soil from the highest to the lowest N-loading treatments, while no significant difference in ammonium concentrations was detected among the different N-loading treatments for the SY and FQ soils. A significantly higher potential nitrification rate (PNR) was observed in the FQ soil while lowest PNR was found in the QY soil. Quantitative PCR analysis of AOB amoA genes demonstrated that AOB abundance was significantly higher in the high N-loading treatments than in the control for the QY soil only, while no significant difference among treatments in the SY and FQ soils. A significant positive correlation between PNR and AOB amoA abundance, however, was found for the SY and FQ soils, but not for the QY soil. Little difference in AOA amoA abundance between different Nloading treatments was observed for all the soils. Conclusions This study suggested that ammonia oxidation capacity in the FQ and SY soils was higher than those in the QY soil with the addition of ammonium fertilizer for a shortterm. These findings indicated that understanding the differential responses of biological nitrification to varying input levels of ammonium fertilizer is important for maximizing N use efficiency and thereby improving agricultural fertilization management.

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Section: Discussionsupporting

confidence: 91%

Contrasting response of nitrification capacity in three agricultural soils to N addition during short-term incubation

Shen

Zhang

2014

J Soils Sediments

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“…3a), which was in agreement with previous studies in a large number of agricultural soils (Wu et al 2011;Habteselassie et al 2013;Chen et al 2015). It has been reported that AOB growth is favored by high-N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected (Di et al 2009(Di et al , 2010a. The stronger suppression of the AOB population size by C 2 H 2 than DCD was observed, suggesting a coincidence of dynamics between the AOB population and N 2 O emission.…”

Section: Nitrosospira Nitrososmonassupporting

confidence: 92%

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Wang

Zhang

Shen

et al. 2016

Environ Sci Pollut Res

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Ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA) are crucial for N 2 O emission as they carry out the key step of nitrification. Dicyandiamide (DCD) and acetylene (C 2 H 2 ) are typical nitrification inhibitors (NIs), while the comparative effects of these NIs on N 2 O production and ammonia oxidizers' (AOB and AOA) growth are unclear. Four treatments including a control, urea, urea + DCD, and urea + C 2 H 2 were set up to investigate their effect of inhibiting soil nitrification, nitrification-related N 2 O emission as well as the growth of ammonia oxidizers with a fluvo-aquic soil using microcosms for 28 days. N 2 O emission and net nitrification rate increased after the application of urea, but were significantly restrained in urea + NI treatments, while C 2 H 2 was more effective in reducing N 2 O emission and nitrification rate than DCD. The abundance of AOB, which was significantly correlated with N 2 O emission and net nitrification rate, was more inhibited by C 2 H 2 than DCD. Furthermore, the application of urea in all the soils had little impact on the AOA community, while obvious shifts of AOB community structure were found compared with the control. All AOB sequences fell within Nitrosospira cluster 3, and the AOA community was clustered to group 1.1b. Collectively, it indicated that application of urea combined with NIs (DCD or C 2 H 2 ) could potentially alter N 2 O emission, mainly through regulating the growth of AOB but not AOA in this fluvo-aquic soil.

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“…AOA did not respond to the application of NH 4 + in the alluvial soil, while it was inhibited with respect to the control in the red soil, thus confirming what it was obtained in agricultural soils (Wu et al 2011;Ai et al 2013;Habteselassie et al 2013;Di et al 2014Di et al , 2014Chen et al 2015). Generally, AOB growth is favored by high NH 4 + -N soil conditions (Jia and Conrad 2009;Verhamme et al 2011;Taylor et al 2012), whereas AOA growth is not affected or suppressed (Di et al 2009(Di et al , 2010Di and Cameron 2011;Di et al 2014). Actually, the AOA population only grew in the control in the red soil, where NH 4 + was generated from mineralised organic N, suggesting that AOA growth is favored in low fertility status soils (Nicol et al 2008;Di et al 2009Di et al , 2010Zhang et al 2012).…”

Section: Discussionsupporting

confidence: 72%

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

et al. 2016

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Nitrification is believed to be one of the major sources of N 2 O production emitted from soil. Previous studies showed that both ammonia-oxidising bacteria (AOB) and archaea (AOA) can produce N 2 O via nitrification but their relative contributions are still poorly defined. Here, we used acetylene, an inhibitor of AOB and AOA ammonia monooxygenase (AMO), and 1-octyne, a selective inhibitor that specifically inhibits AOB AMO, to investigate how AOB versus AOA contribute to N 2 O emissions in two distinct arable soils. Soil amended with ammonium (NH 4 + ) increased N 2 O emissions to a greater extent than nitrate (NO 3 − ), and acetylene had a greater impact on N 2 O emissions in NH 4 + -treated soils than that in NO 3 − -amended soils, which indicated that nitrification was the dominant N 2 O emitting process in these two arable soils. In the alluvial and red soil, the percentage of evolved N 2 O after application of NH 4 + by AOB were 70.5~78.1 % and 18.7~19.7 % by AOA, respectively. Quantitative PCR revealed that NH 4 + addition stimulated AOB growth, and the growth could be significantly inhibited by acetylene or 1-octyne in the two soils. The stimulation of N 2 O emissions by NH 4 + and the relative suppression by inhibitors paralleled fluctuations in the AOB growth. In addition, cumulative N 2 O emissions were not correlated with AOA abundance in the two soils. Our results revealed that AOB could contribute more to soil N 2 O production than AOA in the NH 4 + -amended arable soils.

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Dynamics of ammonia-oxidizing archaea and bacteria populations and contributions to soil nitrification potentials

Cited by 164 publications

References 29 publications

Contrasting response of nitrification capacity in three agricultural soils to N addition during short-term incubation

Contrasting response of nitrification capacity in three agricultural soils to N addition during short-term incubation

Effects of dicyandiamide and acetylene on N2O emissions and ammonia oxidizers in a fluvo-aquic soil applied with urea

Nitrogen fertiliser-induced changes in N2O emissions are attributed more to ammonia-oxidising bacteria rather than archaea as revealed using 1-octyne and acetylene inhibitors in two arable soils

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