Dynamics of amyloid-like aggregation and gel formation of hen egg-white lysozyme in highly concentrated ethanol solution

Dueramae, Isala; Fukuzawa, Shingo; Shinyashiki, Naoki; Yagihara, Shin; Kita, R.

doi:10.17106/jbr.31.21

Cited by 3 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the gelation time needed to be identified to ensure the formation of a three-dimensional network. Several techniques, such as light scattering (LS) [12,33], beam diffraction [13], and rheology [34][35][36], have been widely used to elucidate the mechanism of material gelation and its molecular structure. In this work, the rheological technique was used to investigate the crosslinking behavior due to its direct correlation with the evolving physical and mechanical properties of the system during the crosslinking process.…”

Section: Uv-induced Sol-to-gel Transition Of Pnipam Solutionmentioning

confidence: 99%

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

Dueramae,

Tanaka,

Shinyashiki

et al. 2023

Gels

Self Cite

View full text Add to dashboard Cite

High-performance properties of interpenetration polymer network (IPN) hydrogels, based on physically crosslinked chitosan (CS) and chemically crosslinked poly(N-isopropylacrylamide) (PNiPAM), were successfully developed. The IPN of CS/PNiPAM is proposed to overcome the limited mechanical properties of the single CS network. In this study, the viscoelastic behaviors of prepared materials in both solution and gel states were extensively examined, considering the UV exposure time and crosslinker concentration as key factors. The effect of these factors on gel formation, hydrogel structures, thermal stabilities of networks, and HeLa cell adhesion were studied sequentially. The sol–gel transition was effectively demonstrated through the scaling law, which agrees well with Winter and Chambon’s theory. By subjecting the CS hydrogel to the process operation in an ethanol solution, its properties can be significantly enhanced with increased crosslinker concentration, including the shear modulus, crosslinking degree, gel strength, and thermal stability in its swollen state. The IPN samples exhibit a smooth and dense surface with irregular pores, allowing for much water absorption. The HeLa cells were adhered to and killed using the CS surface cationic charges and then released through hydrolysis by utilizing the hydrophilic/hydrophobic switchable property or thermo-reversible gelation of the PNiPAM network. The results demonstrated that IPN is a highly attractive candidate for anti-fouling materials.

show abstract

Section: Uv-induced Sol-to-gel Transition Of Pnipam Solutionmentioning

confidence: 99%

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

Dueramae,

Tanaka,

Shinyashiki

et al. 2023

Gels

Self Cite

View full text Add to dashboard Cite

show abstract

“…Among proteins involved in FRA is human lysozyme protein . Here we use lysozyme analogue from chicken egg that is relevant to the disease‐related protein. We study nonlinear optical response—two‐photon excited lasing—of Coumarin 307 in the context of lysozyme aggregation.…”

Section: Introductionmentioning

confidence: 99%

Two‐photon excited lasing of Coumarin 307 for lysozyme amyloid fibrils detection

Hańczyc

Procyk

Radzewicz

et al. 2019

Journal of Biophotonics

View full text Add to dashboard Cite

Amyloid fibrils are a well‐recognized hallmark of neurodegeneration. A common approach to detect amyloid fibrils is staining with organic molecules and monitoring optical properties using fluorescence spectroscopy. However, the structural diversity of amyloids necessitates new sensitive methods and probes that can be reliably used to characterize them. Here, Coumarin 307 is applied for lysozyme fibrils detection by observation of laser action in the process of two‐photon excited stimulated emission. It is shown that the lasing threshold and spectrum significantly depend on the adopted structure (α‐helix or β‐sheet) of the lysozyme protein, whereas fluorescence spectrum is insensitive to the protein structure. The applications of coherent stimulated emission light that can be emitted deep inside a scattering medium can be particularly promising for imaging and therapeutic purposes in the neurodegeneration field. Two‐photon excitation with the near‐infrared light, which allows the deepest penetration of tissues, is an important advantage of the method.

show abstract

UV-Crosslinked poly(N-Isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implementing as Anti-fouling Materials

Dueramae,

Tanaka,

Shinyashiki

et al. 2023

Preprint

View full text Add to dashboard Cite

High-performance properties of interpenetration polymer network (IPN) hydrogels, based on physically crosslinked chitosan (CS) and chemically crosslinked poly(N-isopropylacrylamide) (PNiPAM), have been successfully developed. The IPN of CS/PNiPAM is proposed to overcome the limited mechanical properties of the single CS network. In this study, the viscoelastic behaviors of prepared materials in both solution and gel states have been extensively examined, considering the UV exposure time and crosslinker concentration as key factors. The effect of these factors on gel formation, hydrogel structures, thermal stabilities of networks, and HeLa cell adhesion were studied sequentially. The sol-gel transition was effectively demonstrated through the scaling law, which agrees well with Winter and Chambon's theory. By subjecting the CS hydrogel to the process operation in an ethanol solution, its properties can be significantly enhanced with increased crosslinker concentration, including the shear modulus, crosslinking degree, gel strength, and thermal stability in its swollen state. The IPN samples exhibit a smooth and dense surface with irregular pores, allowing for much water absorption. The HeLa cells were adhered to and killed using the CS surface cationic derivative, then released through hydrolysis by utilizing the hydrophilic/hydrophobic switchable property or thermo-reversible gelation of the PNiPAM network. The results demonstrated that IPN is a highly attractive candidate for anti-fouling materials.

show abstract

Dynamics of amyloid-like aggregation and gel formation of hen egg-white lysozyme in highly concentrated ethanol solution

Cited by 3 publications

References 47 publications

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

UV-Crosslinked Poly(N-isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implemented as Anti-Fouling Materials

Two‐photon excited lasing of Coumarin 307 for lysozyme amyloid fibrils detection

UV-Crosslinked poly(N-Isopropylacrylamide) Interpenetrated into Chitosan Structure with Enhancement of Mechanical Properties Implementing as Anti-fouling Materials

Contact Info

Product

Resources

About