Dynamics of aqueous peptide solutions in folded and disordered states examined by dynamic light scattering and dielectric spectroscopy

Melillo, Jorge H.; Gabriel, Jan Philipp; Pabst, Florian; Blochowicz, Thomas; Cerveny, Silvina

doi:10.1039/d1cp01893k

Cited by 6 publications

(3 citation statements)

References 55 publications

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“…To this end, EDLS investigations could be effectively coupled with dielectric measurements (DS). DS, probing the reorientation of permanent molecular dipoles, can detect the rotational dynamics of both solute and solvent [59,60]. It will represent a powerful complementary tool to EDLS for studying hydration properties in extremely viscous media.…”

Section: Discussionmentioning

confidence: 99%

Protein Hydration in a Bioprotecting Mixture

Corezzi

Bracco

Sassi

et al. 2021

Life

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We combined broad-band depolarized light scattering and infrared spectroscopies to study the properties of hydration water in a lysozyme-trehalose aqueous solution, where trehalose is present above the concentration threshold (30% in weight) relevant for biopreservation. The joint use of the two different techniques, which were sensitive to inter-and intra-molecular degrees of freedom, shed new light on the molecular mechanism underlying the interaction between the three species in the mixture. Thanks to the comparison with the binary solution cases, we were able to show that, under the investigated conditions, the protein, through preferential hydration, remains strongly hydrated even in the ternary mixture. This supported the water entrapment scenario, for which a certain amount of water between protein and sugar protects the biomolecule from damage caused by external agents.

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Section: Discussionmentioning

confidence: 99%

Protein Hydration in a Bioprotecting Mixture

Corezzi

Bracco

Sassi

et al. 2021

Life

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“…In addition, we have applied the same temperature profile as that used in the calorimetric measurements. The dynamics of amorphous (no annealing) 3PG-and e-PLL-water solutions were previously analysed [22,23,[39][40][41][42], and a summary of these results can be seen in Figures S1 to S4 of SI (Supplementary Information). For the 3PG solution [1,39], two dynamic processes (the slower one related to the a-relaxation and the faster due to the reorientation of water molecules) are displayed.…”

Section: Dynamical Characterizationmentioning

confidence: 99%

Influence of ice formation on the dynamic and thermodynamic properties of aqueous solutions

2022

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“…Understanding SCW formation is crucial in various technological processes 3 – 5 , usually to avoid undesired ice formation. SCW at ambient pressure forms below the freezing point, where pure water can be prevented from freezing for long periods, e.g., in hard confinement 6 or in soft confinement 7 – 9 . SCW becomes progressively less stable at lower temperatures.…”

Section: Introductionmentioning

confidence: 99%

Electron microscopy and calorimetry of proteins in supercooled water

Melillo

Nikulina

Iriarte-Alonso

et al. 2022

Sci Rep

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Some of the best nucleating agents in nature are ice-nucleating proteins, which boost ice growth better than any other material. They can induce immersion freezing of supercooled water only a few degrees below 0 °C. An open question is whether this ability also extends to the deposition mode, i.e., to water vapor. In this work, we used three proteins, apoferritin, InaZ (ice nucleation active protein Z), and myoglobin, of which the first two are classified as ice-nucleating proteins for the immersion freezing mode. We studied the ice nucleation ability of these proteins by differential scanning calorimetry (immersion freezing) and by environmental scanning electron microscopy (deposition freezing). Our data show that InaZ crystallizes water directly from the vapor phase, while apoferritin first condenses water in the supercooled state, and subsequently crystallizes it, just as myoglobin, which is unable to nucleate ice.

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Dynamics of aqueous peptide solutions in folded and disordered states examined by dynamic light scattering and dielectric spectroscopy

Abstract: Characterizing the segmental dynamics of proteins, and intrinsically disordered proteins in particular, is a challenge in biophysics. In this study, by combining data from broadband dielectric spectroscopy (BDS) and both...

Cited by 6 publications

References 55 publications

Protein Hydration in a Bioprotecting Mixture

Protein Hydration in a Bioprotecting Mixture

Influence of ice formation on the dynamic and thermodynamic properties of aqueous solutions

Electron microscopy and calorimetry of proteins in supercooled water

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