Dynamics of Asymmetric and Symmetric Divisions of Muscle Stem Cells In Vivo and on Artificial Niches

Evano, Brendan; Khalilian, Sara; Carrou, Gilles Le; Almouzni, Geneviève; Tajbakhsh, Shahragim

doi:10.1016/j.celrep.2020.01.097

Cited by 51 publications

(36 citation statements)

References 76 publications

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“…During induced asymmetric divisions of these embryonic stem cells, parental histone H3 and H4 was selectively segregated to the daughter stem cells, while newly synthesized histone H3 and H4 was enriched toward the epiblast stem daughter cell (Ma et al 2020). This pattern of histone (Evano et al 2020) distribution was similar to that found in the Drosophila male germline stem cells under asymmetric cell division. The asymmetric H3 and H4 inheritance mode may serve as a more general mechanism during the asymmetric cell division of stem cell in Drosophila and mouse embryonic stem cell, and in germline stem cell and embryonic stem cell.…”

Section: Dual-color Switching Systems and Pulse-chase Labeling Approasupporting

confidence: 72%

“…The finding that MCM2 and Dpb3/Dpb4, both classical DNA replication machinery components, are involved in the transfer and allocation of parental histones has broadened our understanding of this important cellular process and its role in epigenetic inheritance. Different parental histone allocation patterns have been reported in Drosophila and mouse embryonic stem cells compared with mouse muscle stem cells, using dual-color switching methods and SNAP tag (Evano et al 2020;Ma et al 2020;Tran et al 2012;Wooten et al 2019).…”

Section: Discussionmentioning

confidence: 99%

“…However, unlike in Drosophila germline stem cells and mouse embryonic stem cells, the parental histones were evenly distributed between the muscle daughter cells (Fig. 2b) (Evano et al 2020). The difference observed between Drosophila germline stem cells and mouse embryonic stem cells may be due to the differences in the approaches used, or mechanistic differences between germline stem cells and embryonic stem cells versus somatic stem cells.…”

Section: Dual-color Switching Systems and Pulse-chase Labeling Approamentioning

confidence: 90%

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Chromatin replication and parental histone allocation

Wen

Yao

et al. 2021

GENOME INSTAB. DIS.

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The propagation of epigenetic information across cell divisions is crucial to the maintenance of cell identity. During DNA replication, both parental and newly synthesized histones are deposited onto the replicating DNA, where they assemble into nucleosomes. Parental histones bear a variety of post-translational modifications, which constitute crucial epigenetic information, and the pattern of parental and newly synthesized histone allocation is crucial for the determination and maintenance of cell fate in multicellular organisms. The DNA replication-coupled nucleosome assembly process is regulated by multitude of histone chaperones, and recent breakthroughs in next-generation sequencing and super-resolution imaging methods have provided novel insights into the underlying mechanisms. Here, we review recent findings concerning parental histone deposition onto replicating DNA strands and the segregation of these to symmetrically or asymmetrically dividing daughter cells. Furthermore, we discuss the role of the allocation patterns of parental and newly synthesized histones in the determination of cell fate.

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Section: Dual-color Switching Systems and Pulse-chase Labeling Approasupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Dual-color Switching Systems and Pulse-chase Labeling Approamentioning

confidence: 90%

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Chromatin replication and parental histone allocation

Wen

Yao

et al. 2021

GENOME INSTAB. DIS.

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“…We next performed FACS analysis to determine whether the tdTOM + mononucleated fraction could be isolated following tissue injury. As expected, only a few tdTOM + cells were detected at 3 days post-injury, when myogenic cells are known to be maximally proliferating [23,24,48] ( Figure 3B). tdTOM + cells were most abundant at 5 and 10 days post injury, corresponding to the differentiation shift of the transiently amplifying myoblast population.…”

supporting

confidence: 79%

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Benavente-Diaz

Comai

Girolamo

et al. 2020

Preprint

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Background: Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered by the lack of a sensitive reporter to dynamically track cells during differentiation. Results: Here, we report a knock-in mouse line expressing the tdTOMATO fluorescent protein from the endogenous Myogenin locus. Expression of tdTOMATO in MyogntdTom mice recapitulated endogenous Myogenin expression during embryonic muscle formation and adult regeneration and enabled the isolation of the Myogenin+ cell population. We also show that tdTOMATO fluorescence allows tracking of differentiating myoblasts in vitro and by intravital imaging in vivo. Lastly, we monitored by live imaging the cell division dynamics of differentiating myoblasts in vitro and showed that a fraction of the MYOGENIN+ population can undergo one round of cell division, albeit at a much lower frequency than MYOGENIN- myoblasts. Conclusions: We expect that this reporter mouse will be a valuable resource for researchers investigating skeletal muscle biology in developmental and adult contexts.

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“…High-resolution IVM imaging through the PDMS window revealed the movement and division of GFP+ cells at 3 days post-injury ( Fig. 3C, Movie S5), a time point at which muscle stem cells are maximally proliferating (29,31). Importantly, highresolution images could be acquired through PDMS windows with no observable decline in image quality for at least 18 days after injury ( Fig.…”

Section: Ivm Of Muscle Regeneration Through Pdms Imaging Windowsmentioning

confidence: 92%

Longitudinal high-resolution imaging through a flexible intravital imaging window

Jacquemin

Benavente-Diaz

Djaber

et al. 2021

Preprint

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Intravital microscopy (IVM) is a powerful technique that enables imaging of internal tissues at (sub)cellular resolutions in living animals. Here, we present a silicone-based imaging window consisting of a fully flexible, suture-less design that is ideally suited for long-term, longitudinal IVM of growing tissues and tumors. Crucially, we show that this window, without any customization, is suitable for numerous anatomical locations in mice using a rapid and standardized implantation procedure. This low-cost device represents a substantial technological and performance advance that facilitates intravital imaging in diverse contexts in higher organisms, opening new avenues for in vivo imaging of soft and fragile tissues. One-sentence summary: This study presents a versatile, fully flexible imaging window that acts as an implantable transparent "second skin" for small laboratory animal in vivo imaging.

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Dynamics of Asymmetric and Symmetric Divisions of Muscle Stem Cells In Vivo and on Artificial Niches

Cited by 51 publications

References 76 publications

Chromatin replication and parental histone allocation

Chromatin replication and parental histone allocation

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Longitudinal high-resolution imaging through a flexible intravital imaging window

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