Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling– histomorphometrical, immunohistochemical, and molecular analysis

Esteves, Jônatas Caldeira; Marcantonio, Élcio; Faloni, Ana Paula de Souza; Rocha, Fernanda Regina Godoy; Marcantonio, Rosemary Adriana Chiérici; Wilk, Katarzyna; Intini, Giuseppe

doi:10.1186/1479-5876-11-221

Cited by 69 publications

(69 citation statements)

References 23 publications

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“…no. ab13420, validated in rat in Esteves, et al (2013)] diluted 1:2500 at 4°C, followed by incubation with horseradish-peroxidase-conjugated goat anti-mouse-IgG secondary antibody. The immunoblots were visualized by chemiluminescence (Thermo Scientific, Waltham, MA).…”

Section: Methodsmentioning

confidence: 99%

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Increased trabecular bone and improved biomechanics in an osteocalcin null rat model created by CRISPR/Cas9 technology

Lambert

Challa

Niu

et al. 2016

Disease Models &Amp; Mechanisms

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Osteocalcin, also known as bone γ-carboxyglutamate protein (Bglap), is expressed by osteoblasts and is commonly used as a clinical marker of bone turnover. A mouse model of osteocalcin deficiency has implicated osteocalcin as a mediator of changes to the skeleton, endocrine system, reproductive organs and central nervous system. However, differences between mouse and human osteocalcin at both the genome and protein levels have challenged the validity of extrapolating findings from the osteocalcin-deficient mouse model to human disease. The rat osteocalcin (Bglap) gene locus shares greater synteny with that of humans. To further examine the role of osteocalcin in disease, we created a rat model with complete loss of osteocalcin using the CRISPR/Cas9 system. Rat osteocalcin was modified by injection of CRISPR/Cas9 mRNA into the pronuclei of fertilized single cell Sprague-Dawley embryos, and animals were bred to homozygosity and compound heterozygosity for the mutant alleles. Dual-energy X-ray absorptiometry (DXA), glucose tolerance testing (GTT), insulin tolerance testing (ITT), microcomputed tomography (µCT), and a three-point break biomechanical assay were performed on the excised femurs at 5 months of age. Complete loss of osteocalcin resulted in bones with significantly increased trabecular thickness, density and volume. Cortical bone volume and density were not increased in null animals. The bones had improved functional quality as evidenced by an increase in failure load during the biomechanical stress assay. Differences in glucose homeostasis were observed between groups, but there were no differences in body weight or composition. This rat model of complete loss of osteocalcin provides a platform for further understanding the role of osteocalcin in disease, and it is a novel model of increased bone formation with potential utility in osteoporosis and osteoarthritis research.

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Section: Methodsmentioning

confidence: 99%

“…no. ab13420, validated in rat in Esteves et al (2013)] in phosphate-buffered saline solution containing 1% bovine serum albumin. Appropriate secondary antibody (ImmPRESS, Vector Laboratories) was applied and slides were incubated in DAB (3,3′-diaminobenzidine) peroxidase substrate solution (Dako).…”

Section: Methodsmentioning

confidence: 99%

Increased trabecular bone and improved biomechanics in an osteocalcin null rat model created by CRISPR/Cas9 technology

Lambert

Challa

Niu

et al. 2016

Disease Models &Amp; Mechanisms

View full text Add to dashboard Cite

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“…It is characterized by selective cutting of the mineralized tissue without affecting the adjacent soft tissue, even in the case of accidental contact (Esteves et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Consequently, the requirement for superior methods of bone tissue surgery, which would enable less invasive, precise osteotomies unrelated to the geometry of the mechanical instrument, with limited collateral tissue trauma and reduced force and friction, has prompted the development of new osteotomy techniques: erbium laser ablation and piezoelectric surgery (Sulewski, 2000;Frentzen et al, 2003;Stübinger et al, 2007;de Mello et al, 2008;Romeo et al, 2009;Stübinger et al, 2009;Esteves et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Bone Healing Following Different Types of Osteotomy: Scanning Electron Microscopy (SEM) and Three-Dimensional SEM Analyses

et al. 2016

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Abstract:The aim of the present study was to compare dynamics of the bone healing process after different types of osteotomies. In total, 24 Wistar rats were subjected to different types of osteotomy performed with standard steel bur, piezosurgery, contact, and non-contact Erbium:yttrium-aluminum-garnet (Er:YAG) laser ablation. The animals were randomly divided into four groups, to be euthanized immediately after the procedure, or at 1, 2, or 3 weeks after surgery. The obtained bone samples were analyzed by scanning electron microscopy (SEM). Immediately after surgery, there were significant differences in the appearance of the bone defects, with presence of bone fragments and debris after standard steel bur preparation, compared with the clean smooth walls and relatively sharp edges in all other groups. The initial bone formation in defects prepared by piezosurgery was observed to be the most rapid. After 3 weeks, all bone defects were completely restored; although, differences in the healing pattern were noted, with a modest initial delay in healing after laser preparation. The first stage of the bone healing process was delayed when contact and non-contact Er:YAG laser modes were used and accelerated by piezosurgery; however, the results after 3 weeks demonstrated similar restitution of defects in all tested groups.

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“…OPG and RANKL are also used as markers of bone repair 35) . However, steroids suppress OPG production and enhance RANKL expression.…”

mentioning

confidence: 99%

Jawbone Changes in Sodium Zoledronic Acid- and Dexamethasone-Treated Rats

Tezuka

Tanaka

2016

J. Hard Tissue Biology.

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Previous studies have evaluated bisphosphonate-related osteonecrosis of the jaw (ONJ) after tooth extraction with concurrent administration of bisphosphonate zoledronic acid (ZOL) and steroids. However, details of jawbone changes after administration of ZOL and steroids without tooth extraction remain unclear. In this study, we administered ZOL and dexamethasone (DEX) in rats and compared changes in the jawbone with and without tooth extraction. The right maxillary first molar (M 1 ) was extracted from 8-week-old rats treated with ZOL, ZOL+DEX or DEX. The right maxillary M 1 region as the extraction sample and the left maxillary M 1 region as the non-extraction sample were compared with macroscopic, radiological, histologic and immunohistochemical examinations. In the extraction samples, we detected incomplete mucosal closure macroscopically and found ONJ on histological examination. In addition, immunohistochemical and threedimensional analysis of trabecular bone structure showed inhibition of remodeling and angiogenesis in the extraction samples. In the non-extraction samples, we observed inhibition of remodeling, reduced activity of osteoclasts and osteoblasts and suppression of angiogenesis. These findings demonstrate that ONJ can develop under the mucosa with ZOL and DEX treatment, even without invasive procedures. The data suggest that ZOL and DEX can induce ONJ in rats under both invasive and noninvasive conditions because ZOL suppresses osteoclast activity and DEX inhibits remodeling by suppressing osteoblast activity. The results also suggest that surgical stress, such as tooth extraction or bacterial infection, might lead to more severe bisphosphonaterelated ONJ.

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Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling– histomorphometrical, immunohistochemical, and molecular analysis

Cited by 69 publications

References 23 publications

Increased trabecular bone and improved biomechanics in an osteocalcin null rat model created by CRISPR/Cas9 technology

Increased trabecular bone and improved biomechanics in an osteocalcin null rat model created by CRISPR/Cas9 technology

Bone Healing Following Different Types of Osteotomy: Scanning Electron Microscopy (SEM) and Three-Dimensional SEM Analyses

Jawbone Changes in Sodium Zoledronic Acid- and Dexamethasone-Treated Rats

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