Dynamics of capillary electrochromatography experimental study on the electrosmotic flow and conductance in open and packed capillaries

Separation of acidic and basic compounds in capillary electrochromatography with polymethacrylate-based monolithic columns

“…Meanwhile, the electrophoretic flow of acidic compounds goes against EOF, resulting in rather long analysis time or even no peaks when the electrophoretic flow velocity is higher than EOF velocity. The retention behavior of acidic compounds at high pH has been investigated in the literature [20,21]. However, some acidic species with higher electrophoretic mobility than that of EOF cannot be electrokinetically injected into CEC columns.…”

Separation of peptides by strong cation-exchange capillary electrochromatography

“…If not otherwise stated, the sample solution performed on the ion-exchange packings. Smith and was first prepared in water, then diluted to approEvans [5] as well as Choudhary and Horvath [22] priate concentration by the mobile phases containing have reported the separation of basic tricyclic antide-60% acetonitrile in 10 mM KH PO (pH 3.0) before 2 4 pressants and three peptides by IE-CEC with SCX injection. stationary phase.…”

Separation of acidic compounds by strong anion-exchange capillary electrochromatography

“…Therefore the anionic acids will eventualSeveral papers on ion-exchange CEC (IE-CEC) ly elute out from detection window only when the using SCX packings have been reported in literature EOF is greater than the electrophoretic mobility of [11][12][13][14] for separation of cationic compounds, whilé anionic acids. Choudhary and Horvath [2] and research on CEC using strong anion-exchange Moffatt et al [3] have reported the analysis of acidic (SAX) packings is seldom reported. Strong anioncompounds with high pH eluent by RP-CEC.…”