Dynamics of (Cd,Zn)-metallothioneins in gills, liver and kidney of common carp Cyprinus carpio during cadmium exposure

Smet, Hyo Jung De; Wachter, B. De; Lobinski, Richard; Blust, Ronny

doi:10.1016/s0166-445x(00)00136-3

Cited by 119 publications

(57 citation statements)

References 27 publications

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“…Such a saturation of expression is consistent with previous observation on the responses of MTs and other metalloenzymes during acute exposure to relatively high doses of heavy metals (De Smet et al, 2001;Yan and Chan, 2004;Cheung et al, 2005;Cho et al, 2006). It is worth noting that not only metal dose but also duration time should be taken into account (Van Campenhout et al, 2010).…”

Section: The Saturation Of Detoxification Mechanismsupporting

confidence: 91%

“…And the role of MTs in nervous tissue of vertebrates has been suggested (Kramer et al, 1996;Tallkvist et al, 2002). Cd was the most potential for MTs induction among three metals (i.e., Cd, Cu, and Zn) in H. mylodon, and this outcome was consistent with previous discoveries that Cd was one of very powerful inducers for metallothionein transcription in fish (De Smet et al, 2001;Hermesz et al, 2001;Chan et al, 2006;Cho et al, 2008). It had been also suggested that transcriptional regulation of metallothionein in the fingerings of this species should be very sensitive to Cd, and any potential harmful stress to this species origin from the Cd pollution at much lower concentration than 0.1 mM in wild habitats may be detected by tracing the MTs expression in fingerlings if the exposure is extended (Cho et al, 2008).…”

Section: Cadmiumsupporting

confidence: 87%

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Characteristics, functions, and applications of metallothionein in aquatic vertebrates

Weichao

Mao

et al. 2014

Front. Mar. Sci.

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The documents on Metallothioneins (MTs) in aquatic creatures, especially focusing on their function as biomarkers in environmental monitoring programmes, are vast and increasing. There are, however, few papers to summary the physiological role of MTs in aquatic organisms especially on development. The multifaceted roles of MTs include involvement in homeostasis, protection against heavy metals and oxidant damages, and metabolic regulation, sequestration and/or redox control. In this paper, we have collected published information on MTs in aquatic organisms-pisces, amphibians, mammals, etc., and analyzed their function in these aquatic animals. MTs have four main functions in aquatic vertebrate. They are respectively bioaccumulation of toxic metals and detoxification, homeostatic regulation of metals, protection against oxidative stress and neuroprotective mechanism. MTs separate in different tissues and they have various distributions in different tissues of aquatic vertebrate, including liver, gills, kidney, testes, and brain. MTs can be induced by a variety of environmental and physiological factors, among which, heavy metals are the main kind of MTs inducers in aquatic vertebrate. Here we pay more attention on the essential metals copper (Cu) and zinc (Zn) and the non-essential metals cadmium (Cd), silver (Ag), lead (Pb), and mercury (Hg).

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Section: The Saturation Of Detoxification Mechanismsupporting

confidence: 91%

Section: Cadmiumsupporting

confidence: 87%

Characteristics, functions, and applications of metallothionein in aquatic vertebrates

Weichao

Mao

et al. 2014

Front. Mar. Sci.

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“…The induction of de novo synthesis of MTs following cadmium exposure has been described in several studies (Jebali et al, 2006;Ghedira et al, 2010). According to De Smet et al (2001), MT induction following intraperitoneal injection of cadmium, as described by Ghedira et al (2010), is evidence of a genetic ability to synthesise MTs. Contradictory results were reported from a field study conducted by Kovarova et al (2009), where no significant correlation between cadmium liver content and MT concentration was observed.…”

Section: Cadmiummentioning

confidence: 99%

“…Metallothioneins play a major role in the detoxification of cadmium, and this process is clearly organspecific (De Smet et al, 2001). The induction of de novo synthesis of MTs following cadmium exposure has been described in several studies (Jebali et al, 2006;Ghedira et al, 2010).…”

Section: Cadmiummentioning

confidence: 99%

Metals as a cause of oxidative stress in fish: a review

Ševčíková¹,

Modrá²,

Slaninova³

et al. 2011

Vet. Med.

319

123

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ABSTRACT:This review summarizes the current knowledge on the contribution of metals to the development of oxidative stress in fish. Metals are important inducers of oxidative stress in aquatic organisms, promoting formation of reactive oxygen species through two mechanisms. Redox active metals generate reactive oxygen species through redox cycling, while metals without redox potential impair antioxidant defences, especially that of thiol-containing antioxidants and enzymes. Elevated levels of reactive oxygen species lead to oxidative damage including lipid peroxidation, protein and DNA oxidation, and enzyme inactivation. Antioxidant defences include the enzyme system and low molecular weight antioxidants. Metal-binding proteins, such as ferritin, ceruloplasmin and metallothioneins, have special functions in the detoxification of toxic metals and also play a role in the metabolism and homeostasis of essential metals. Recent studies of metallothioneins as biomarkers indicate that quantitative analysis of mRNA expression of metallothionein genes can be appropriate in cases with elevated levels of metals and no evidence of oxidative damage in fish tissue. Components of the antioxidant defence are used as biochemical markers of oxidative stress. These markers may be manifested differently in the field than in results found in laboratory studies. A complex approach should be taken in field studies of metal contamination of the aquatic environment. Keywords: ROS; metallothioneins; glutathion; superoxide dismutase; antioxidant defenceList of abbreviations ALA-D = aminolevulinic acid dehydratase; BNF = β-naphthoflavone; CAT = catalase; GPx = glutathione peroxidise; GR = glutathione reductase; GSH = glutathione; GSSG = glutathione disulphide; GST = glutathione S-transferase; LPO = lipid peroxidation; MDA = malondialdehyde; MTs = metallothioneins; NADPH = nicotinamidadeninedinucleotide phosphate (reduced); ROS = reactive oxygen species; SOD = superoxide dismutase

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“…Among metal ligands, induced MTs are involved in non-enzymatic homeostasis and a detoxifi cation mechanism of fi sh against metal exposure. In previous studies, the induction of MT content in fi sh gills exposed to metals has been documented in tilapia (Oreochromis mossambicus) [43][44], common carp (Cyprinus carpio) [45][46], and catfi sh (Clarias gariepinus) [47]. Our study also demonstrated that waterborne exposure to Cu 2+ could increase MT contents in gills of C. auratus in a concentration-dependent manner, confi rming that MT synthesis level in C. auratus gills is a sensitive biomarker for aquatic pollution of metals.…”

Section: Discussion Of Resultsmentioning

confidence: 96%

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

Lu¹,

Ding

2018

Pol. J. Environ. Stud.

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To evaluate potential interactive effects of metals and polycyclic aromatic hydrocarbons (PAHs) on biomarker responses, groups of the freshwater fi sh crucian carp (Carassius auratus) were exposed to single and binary combinations of copper (Cu) (0.01-0.16 mg L-1) with fl uorene (Fl) or fl uoranthene (Fluo) (2-10 mg kg -1 ) for 96 h. Dose-dependent increases in the activities of phases I and II metabolic enzymes [7-ethoxyresorufi n O-deethylase (EROD) and glutathione-S-transferase (GST), respectively] were observed in fi sh liver exposed to Fl and Fluo, but these enzyme activities did not differ signifi cantly from the controls when co-treated with higher concentrations of Cu, suggesting an inhibiting interaction on the metabolic enzymes. Although Cu did not alter catalase (CAT) activity, CAT activity was decreased in fi sh liver exposed to the two PAHs alone or in combination with Cu. Although metallothionein (MT) content in gills was signifi cantly increased following exposure to Cu alone or in combination with Fl and Fluo, the induction folds of MT decreased under co-exposure. Co-exposures to these chemicals invoked complex biomarker responses in fi sh liver and gills. These results highlight the need for careful consideration of the interactive effects of multiple environmental stressors on fi sh.

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Dynamics of (Cd,Zn)-metallothioneins in gills, liver and kidney of common carp Cyprinus carpio during cadmium exposure

Cited by 119 publications

References 27 publications

Characteristics, functions, and applications of metallothionein in aquatic vertebrates

Characteristics, functions, and applications of metallothionein in aquatic vertebrates

Metals as a cause of oxidative stress in fish: a review

Interactive Effects of Copper, Fluorene, and Fluoranthene on Enzymatic Biomarkers and Metallothionein Levels in Crucian Carp (Carassius auratus)

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