Dynamics of Cellular Components in Meiotic and Premeiotic Divisions in Drosophila Males

Inoué, Yoshihiro; Miyauchi, Chie; Ogata, Tubasa; Kitazawa, Daishi

doi:10.5772/30363

Cited by 2 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, we observed the association of transition proteins and protamines with the disorganized spermatid nuclei in Paf1L mutants, indicating that DNA compaction itself remained unaffected (Figure S5) . To understand whether the defects resulting in faulty spermatid individualization arise before or after completion of meiosis in spermatocytes, we next characterized early spermatids at the ‘nebenkern/onion’ stage, where aberrant nuclear morphology is a known hallmark of meiotic defects in the preceding spermatocyte stage 34 . In control testes, the majority of spermatids exhibited nuclei and nebenkern structures that were spherical, of equal size, and occurred in pairs ( Figure 2E, left) .…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

A germline PAF1 paralog complex ensures cell type-specific gene expression

Vilstrup,

Gupta,

Rasmussen

et al. 2024

Preprint

View full text Add to dashboard Cite

Animal germline development and fertility rely on paralogs of general transcription factors that recruit RNA polymerase II to ensure cell type-specific gene expression. It remains unclear whether gene expression processes downstream of such paralog-based transcription is distinct from that of canonical RNA polymerase II genes. In Drosophila, the testis-specific TBP-associated factors (tTAFs) activate over a thousand spermatocyte-specific gene promoters to enable meiosis and germ cell differentiation. Here, we show that efficient termination of tTAF-activated transcription relies on testis-specific paralogs of canonical Polymerase Associated Factor 1 Complex (PAF1C) proteins, which form a testis-specific PAF1C (tPAF). Consequently, tPAF mutants cause aberrant expression of hundreds of downstream genes due to read-in transcription. Furthermore, tPAF facilitates expression of Y-linked male fertility factor genes, and thus broadly maintains spermatocyte-specific gene expression. Consistently, tPAF is required for the segregation of meiotic chromosomes and male fertility. Supported by comparative in vivo protein interaction assays, we provide a mechanistic model for the functional divergence of tPAF and PAF1C and for transcription termination as a developmentally regulated process required for cell type-specific gene expression.

show abstract

Section: Resultsmentioning

confidence: 99%

“…By contrast, tPAF mutant testes displayed pronounced abnormalities in the majority of spermatid cysts (Figure 2E-F; Figure S4C , F) . Abnormalities include irregular shapes, enlarged nebenkern, and multinucleation – defects which strongly suggest failure in cytokinesis during meiosis 34 .…”

Section: Resultsmentioning

confidence: 99%

A germline PAF1 paralog complex ensures cell type-specific gene expression

Vilstrup,

Gupta,

Rasmussen

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

A germline PAF1 paralog complex ensures cell type-specific gene expression

Vilstrup,

Gupta,

Rasmussen

et al. 2024

Genes Dev.

View full text Add to dashboard Cite

Animal germline development and fertility rely on paralogs of general transcription factors that recruit RNA polymerase II to ensure cell type-specific gene expression. It remains unclear whether gene expression processes downstream from such paralog-based transcription is distinct from that of canonical RNA polymerase II genes. InDrosophila, the testis-specific TBP-associated factors (tTAFs) activate over a thousand spermatocyte-specific gene promoters to enable meiosis and germ cell differentiation. Here, we show that efficient termination of tTAF-activated transcription relies on testis-specific paralogs of canonical polymerase-associated factor 1 complex (PAF1C) proteins, which form a testis-specific PAF1C (tPAF). Consequently, tPAF mutants show aberrant expression of hundreds of downstream genes due to read-in transcription. Furthermore, tPAF facilitates expression of Y-linked male fertility factor genes and thus serves to maintain spermatocyte-specific gene expression. Consistently, tPAF is required for the segregation of meiotic chromosomes and male fertility. Supported by comparative in vivo protein interaction assays, we provide a mechanistic model for the functional divergence of tPAF and the PAF1C and identify transcription termination as a developmentally regulated process required for germline-specific gene expression.

show abstract

Dynamics of Cellular Components in Meiotic and Premeiotic Divisions in Drosophila Males

Cited by 2 publications

References 47 publications

A germline PAF1 paralog complex ensures cell type-specific gene expression

A germline PAF1 paralog complex ensures cell type-specific gene expression

A germline PAF1 paralog complex ensures cell type-specific gene expression

Contact Info

Product

Resources

About