Dynamics of DNA Methylation and DNMT Expression During Gametogenesis and Early Development of Scallop Patinopecten yessoensis

Li, Yangping; Zhang, Lingling; Li, Yajuan; Li, Wanru; Guo, Zhenyi; Li, Ruojiao; Hu, Xiaoli; Bao, Zhenmin; Wang, Shi

doi:10.1007/s10126-018-09871-w

Cited by 31 publications

(19 citation statements)

References 56 publications

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“…As a common epigenetic phenomenon, changes in DNA methylation can regulate gene expression (Costello et al 1994; Futscher et al 2002; Liu and Wendel 2003; Ma and Gustafson 2005; Koganti et al 2017; Pang et al 2015). DNA methylation is closely related to gametogenesis, embryonic development, sex differentiation, and sex determination (Li et al 2019; Wei et al 2018; Jiang et al 2015; Gupta et al 2012; Piferrer et al 2012). In this study, compared with diploid ancestors, the Ph1 and Dmc1 genes are hypomethylated in all 3nCC individuals and female 3nRR individuals, while hypermethylated in male 3nRR individuals.…”

Section: Discussionmentioning

confidence: 99%

Analysis on the Meiosis-Related Gene (Dmc1, Ph1) Expression in Autotriploid Carassius auratus

et al. 2019

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Triploid is usually considered to be unable to perform normal meiosis due to the abnormal behavior of the three sets of chromosomes. But autotriploid Carassius auratus in the Dongting water system (3n = 150, abbreviated as 3nCC) can perform normal meiosis. In artificial autotriploid Carassius auratus (3n = 150, abbreviated as 3nRR), female individuals undergo normal meiosis and produce mature gametes, while male individuals cannot. To better understand the effects of triploidization on meiosis in fish, we study the structure, methylation level, and expression level of meiosis-related genes (Dmc1, Ph1) in diploid Carassius auratus (2n = 100, abbreviated as 2nCC), Carassius auratus red var.(2n = 100, abbreviated as RCC), 3nCC and 3nRR. The results show that, compared with their diploid ancestors (2nCC and RCC), Dmc1 and Ph1 genes are hypomethylated in all 3nCC and female 3nRR, while are hypermethylated in male 3nRR. Correspondingly, Dmc1 and Ph1 genes are highly expressed in all 3nCC and female 3nRR, while are lowly expressed in male 3nRR. These results indicate that high expression of meiosis-related genes can contribute to restoration of bivalent pairing during meiosis in autotriploid Carassius auratus. This study provides new insights into the effect of DNA methylation on the fertility in triploid fish.

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Section: Discussionmentioning

confidence: 99%

Analysis on the Meiosis-Related Gene (Dmc1, Ph1) Expression in Autotriploid Carassius auratus

et al. 2019

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“…Parental conditioning for the benefit of production (Utting and Millican, 1997) has merit for economically important bivalves (Parker et al ., 2012; 2013; 2015). Advancements in genome sequencing demands further research to synthesize-omic profiling (i.e global DNA methylation and differential expression) with physiological responses throughout reproductive and offspring development under environmental stress (Gavery and Roberts, 2014; Li et al , 2019) to determine if these mechanisms are transferable among species. Stress conditioning within a generation at critical life stages may yield beneficial responses for food production and provide a baseline for other long-lived burrowing bivalves of ecological and economic importance.…”

Section: Resultsmentioning

confidence: 99%

Metabolic Recovery and Compensatory Shell Growth of Juvenile Pacific Geoduck Panopea Generosa Following Short-Term Exposure to Acidified Seawater

Gurr

Vadopalas

Roberts

et al. 2019

Preprint

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AbstractWhile acute stressors can be detrimental, environmental stress conditioning can improve performance. To test the hypothesis that physiological status is altered by stress conditioning, we subjected juvenile Pacific geoduck, Panopea generosa, to repeated exposures of elevated pCO2 in a commercial hatchery setting followed by a period in ambient common garden. Respiration rate and shell length were measured for juvenile geoduck periodically throughout short-term repeated reciprocal exposure periods in ambient (~550 µatm) or elevated (~2400 µatm) pCO2 treatments and in common, ambient conditions, five months after exposure. Short-term exposure periods comprised an initial 10-day exposure followed by 14 days in ambient before a secondary 6-day reciprocal exposure. The initial exposure to elevated pCO2 significantly reduced respiration rate by 25% relative to ambient conditions, but no effect on shell growth was detected. Following 14 days in common garden, ambient conditions, reciprocal exposure to elevated or ambient pCO2 did not alter juvenile respiration rates, indicating ability for metabolic recovery under subsequent conditions. Shell growth was negatively affected during the reciprocal treatment in both exposure histories, however clams exposed to the initial elevated pCO2 showed compensatory growth with 5.8% greater shell length (on average between the two secondary exposures) after five months in ambient conditions. Additionally, clams exposed to the secondary elevated pCO2 showed 52.4% increase in respiration rate after five months in ambient conditions. Early exposure to low pH appears to trigger carry-over effects suggesting bioenergetic re-allocation facilitates growth compensation. Life stage-specific exposures to stress can determine when it may be especially detrimental, or advantageous, to apply stress conditioning for commercial production of this long-lived burrowing clam.Lay summaryCommercial shellfish hatcheries provide essential food security, but often production can be hampered by sensitivity of shellfish at early life stages. Repeated short-term exposures can increase tolerance and performance of the geoduck clam with implications for sustainable aquaculture.

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“…This reminds of the global erasure of histone marks observed during the reprogramming event in the zebrafish and the mouse [42,43,44,45]. However, as for DNA methylation [25,46], patterns of histone marks reveal both similarities and differences with vertebrates and highlight the distinct reprogramming events happening among species [43,47,48,49]. Indeed, global methylation of H3K27 appears to be stable during oyster early development, while in vertebrates, H3K27me3 is erased after fertilization before being deposited again in later stages [43,44,45].…”

Section: Discussionmentioning

confidence: 99%

Histone Methylation Participates in Gene Expression Control during the Early Development of the Pacific Oyster Crassostrea gigas

Fellous

Lefranc

Jouaux

et al. 2019

Genes

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Histone methylation patterns are important epigenetic regulators of mammalian development, notably through stem cell identity maintenance by chromatin remodeling and transcriptional control of pluripotency genes. But, the implications of histone marks are poorly understood in distant groups outside vertebrates and ecdysozoan models. However, the development of the Pacific oyster Crassostrea gigas is under the strong epigenetic influence of DNA methylation, and Jumonji histone-demethylase orthologues are highly expressed during C. gigas early life. This suggests a physiological relevance of histone methylation regulation in oyster development, raising the question of functional conservation of this epigenetic pathway in lophotrochozoan. Quantification of histone methylation using fluorescent ELISAs during oyster early life indicated significant variations in monomethyl histone H3 lysine 4 (H3K4me), an overall decrease in H3K9 mono- and tri-methylations, and in H3K36 methylations, respectively, whereas no significant modification could be detected in H3K27 methylation. Early in vivo treatment with the JmjC-specific inhibitor Methylstat induced hypermethylation of all the examined histone H3 lysines and developmental alterations as revealed by scanning electronic microscopy. Using microarrays, we identified 376 genes that were differentially expressed under methylstat treatment, which expression patterns could discriminate between samples as indicated by principal component analysis. Furthermore, Gene Ontology revealed that these genes were related to processes potentially important for embryonic stages such as binding, cell differentiation and development. These results suggest an important physiological significance of histone methylation in the oyster embryonic and larval life, providing, to our knowledge, the first insights into epigenetic regulation by histone methylation in lophotrochozoan development.

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Dynamics of DNA Methylation and DNMT Expression During Gametogenesis and Early Development of Scallop Patinopecten yessoensis

Cited by 31 publications

References 56 publications

Analysis on the Meiosis-Related Gene (Dmc1, Ph1) Expression in Autotriploid Carassius auratus

Analysis on the Meiosis-Related Gene (Dmc1, Ph1) Expression in Autotriploid Carassius auratus

Metabolic Recovery and Compensatory Shell Growth of Juvenile Pacific Geoduck Panopea Generosa Following Short-Term Exposure to Acidified Seawater

Histone Methylation Participates in Gene Expression Control during the Early Development of the Pacific Oyster Crassostrea gigas

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