Dynamics of DNA Supercoiling

Modeling and Analysis of Intercalant Effects on Circular DNA Conformation

“…Being constrained, intercalation into circular DNA causes localized unwinding of the molecule, which subsequently causes a structural transformation according to the Calugareanu−Fuller−White model. 29 EtBr is a very popular prototypical intercalating molecule that has been used for several decades to fluorescently label DNA and alter its mobility during electrophoresis. 30 EtBr's interaction with DNA has been well-characterized experimentally and serves as a good model for anthracyclines, such as daunorubicin and doxorubicin.…”

“…Circular covalently closed DNA has been shown to be a good model for representing the structure of DNA in vivo ,, because it contains the constraints of closed domains found in DNA that give rise to its large-scale conformational properties. Being constrained, intercalation into circular DNA causes localized unwinding of the molecule, which subsequently causes a structural transformation according to the Calugareanu–Fuller–White model . EtBr is a very popular prototypical intercalating molecule that has been used for several decades to fluorescently label DNA and alter its mobility during electrophoresis .…”