Dynamics of Expression and Maturation of the Type III Secretion System of Enteropathogenic Escherichia coli

Yerushalmi, Gal; Litvak, Yael; Gur-Arie, Lihi; Rosenshine, Ilan

doi:10.1128/jb.00069-14

Cited by 26 publications

(29 citation statements)

References 31 publications

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“…The ability of EPEC to induce A/E lesions is related to a pathogenicity island (PAI) of 35 kb called the locus of enterocyte effacement (LEE) [14]. The LEE comprises 41 genes organized in 5 principal operons (LEE1-LEE5) and several smaller transcriptional units (Figure 2) [15,16]. Orthologues of LEE are also found in other members of A/E pathogens [11].…”

Section: Epec Virulence Factors 21 a Pathogenicity Island Called Leementioning

confidence: 99%

“…EPEC tightly regulates its virulence genes in response to environmental conditions such as temperature [16], the increase of the pH of the small intestine [33, 34], and some hormones which are released during stress conditions [20]. Upon EPEC interaction with enterocytes, EspB and EspD proteins are inserted into the host cell membrane and assemble to form a translocation pore [25,26].…”

Section: Epec Pathogenesismentioning

confidence: 99%

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Systematic Deletion of Type III Secretion System Effectors in Enteropathogenic E. coli Unveils the Role of Non-LEE Effectors in A/E Lesion Formation

Cepeda-Molero¹,

Schüller

Frankel

et al. 2020

E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment

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Enteropathogenic E. coli (EPEC) is a diarrheagenic human pathogen. The hallmark of EPEC infection is the formation of the attaching and effacing (A/E) lesion in the intestinal epithelial cells, characterized by the effacement of brush border microvilli and the intimate bacterial attachment to the enterocyte in actinrich pedestal-like structures. The locus of enterocyte effacement (LEE) in the EPEC genome encodes a type III protein secretion system (T3SS) that translocates multiple effector proteins into the host cell to subvert cellular functions for the benefit of the pathogen. These effectors are encoded both within and outside the LEE. In vitro cell culture infections have shown that LEE effectors are required for intimate bacterial attachment to the epithelial cells, whereas non-LEE effectors mostly play a role in modulating inflammation and cell apoptosis in the gut epithelium. We constructed a set of EPEC mutant strains harboring deletions in the complete repertoire of genes encoding T3SS effectors. Infection of human intestinal in vitro organ cultures (IVOC) with these mutant strains surprisingly revealed that non-LEE effectors are also needed to induce efficient A/E lesion formation in the intestinal mucosal tissue.

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Section: Epec Virulence Factors 21 a Pathogenicity Island Called Leementioning

confidence: 99%

Section: Epec Pathogenesismentioning

confidence: 99%

Systematic Deletion of Type III Secretion System Effectors in Enteropathogenic E. coli Unveils the Role of Non-LEE Effectors in A/E Lesion Formation

Cepeda-Molero¹,

Schüller

Frankel

et al. 2020

E. Coli Infections - Importance of Early Diagnosis and Efficient Treatment

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“…A região LEE é organizada em cinco operons principais (LEE1 a LEE5) e outras unidades transcricionais menores (YERUSHALMI et al, 2014). LEE1, LEE2, LEE3 e LEE7 expressam componentes de um sistema de secreção tipo III (TTSS) e reguladores da região LEE (LEE encoded regulator -Ler, Global Regulator of LEE-Activator -GrlA, Global Regulator of LEE-Repressor -GrlR) (ELLIOTT et al, 2000).…”

Section: Lesão Attaching and Effacingunclassified

Influência dos sistemas de Quorum Sensing Al-1/Al2/3Al-3 nos fatores de virulência de EPEC atípica de origem animal

Couto¹

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The secretion of low molecular weight signaling molecules that accumulate in the extracellular medium until reaching a critical concentration limit for its detection, leading to intracellular signaling and effector responses, defines the bacterial communication system called Quorum Sensing. This system regulates collective behaviors and has been proven to be a widespread mechanism in several bacterial species. Several studies have described the existence of at least 3 Quorum Sensing-related systems in Gram-negative bacteria, LuxIR/AI-1, LuxS/AI-2 and QseBC/AI-3/Epinephrine/Norepinephrine. Virulence factors such as biofilm formation, motility and adhesion to the host epithelium should be adequately regulated to make the most of the situation in which the bacterium is found. The aim of this work was to analyze the influence and correlation of luxS, qseC and sdiA genes, related to the bacterial communication system Quorum Sensing, on the main virulence factors of an atypical EPEC strain isolated from an animal. Mutants were obtained through homologous recombination mediated by the Lambda Red system, and then were submitted to phenotypic assays. AI-2 signaling and luxS play roles in motility, biofilm formation, and adhesion to epithelial cells. QseC influences the production of biofilm by the regulation of components of the extracellular matrix, and participates in the processes of motility and adhesion as well. The epinephrine hormone alters the processes of motility, biofilm formation and development of the A/E lesion. The sdiA gene also plays an important role in the regulation of virulence factors, even in the absence of AHL. An antagonistic interaction seems to exist between the qseC and luxS genes. The absence of Quorum Sensing systems promotes the production of a robust biofilm in the AP155 strain.

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“…Outro gene com expressão diminuída foi o CV_2610 codificador da proteína EscU. Em E. coli, EscU é homóloga às proteínas YscU/FlhB, que são proteínas de membrana interna do T3SS e influenciam o transporte de efetores (THOMASSIN;HE;THOMAS, 2011;YERUSHALMI et al, 2014). No entanto, a ausência de EscU não impede a formação do T3SS (YERUSHALMI et al, 2014).…”

Section: Identificação Dos Genes Regulados Por Cv_1810unclassified

Caracterização funcional de fatores de transcrição da família MarR de Chromobacterium violaceum

Mello¹

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pela excelente orientação, dedicação, ensinamentos, confiança, compreensão, companhia e principalmente pelas longas e quase diárias conversas sobre nosso trabalho. Aos amigos de laboratório Kelly, Juliana, Greicy, Bianca, Júlia, Renato, Carlos e Waldir e amigos de corredor Relber, Flávia e Lívia pela feliz convivência diária, risadas, celebrações e aprendizado. À técnica Claudia R. M. Faggion por todo auxílio, preocupação, risadas e conversas gostosas. A todos os docentes, funcionários e alunos da USP-Ribeirão Preto que compartilharam conhecimento comigo. A todos os alunos que passaram pelo laboratório e me deram a oportunidade de ensinar e aprender durante os cursos de verão, iniciação científica ou matéria do curso de Ciências Biomédicas. Às secretarias Gabriela B. Zamoner, Lúcia H. P. Raphael e Camila Fabris, pela competência e atenção ao me ajudarem nos processos burocráticos.

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Dynamics of Expression and Maturation of the Type III Secretion System of Enteropathogenic Escherichia coli

Cited by 26 publications

References 31 publications

Systematic Deletion of Type III Secretion System Effectors in Enteropathogenic E. coli Unveils the Role of Non-LEE Effectors in A/E Lesion Formation

Systematic Deletion of Type III Secretion System Effectors in Enteropathogenic E. coli Unveils the Role of Non-LEE Effectors in A/E Lesion Formation

Influência dos sistemas de Quorum Sensing Al-1/Al2/3Al-3 nos fatores de virulência de EPEC atípica de origem animal

Caracterização funcional de fatores de transcrição da família MarR de Chromobacterium violaceum

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