Dynamics of human renal tumor colony growth in vitro

Abstract

Two-layer soft agar cultures from 26 patients with renal cell carcinoma, 21 renal primary lesions and 5 metastatic lesions, were evaluated for tumor colony formation using both dynamic growth curves and static single time point colony counting. Dynamic growth curves markedly increased the number of evaluable tumor cultures. There was no relationship between colony formation and TNM stage of the tumor or renal vein invasion. However, there was a significantly (p less than 0.02) higher rate of colony formation from younger (less than 50 y.) patients than from older patients. Only 2 of 5 tumors tested showed response to one or more cytostatic agents in vitro, both tumors showing response to Adriamycin and one to Cis-platin. Dynamic evaluation of tumor colony formation in soft agar may increase the clinical applicability of the human tumor cloning system both by increasing the number of evaluable cultures and by providing more information about the processes involved in tumor colony formation in vitro.

Establishment and characterization of seven dunning rat prostatic cancer cell lines and their use in developing methods for predicting metastatic abilities of prostatic cancers

“…As a control for the baseline of growth, one set of dishes was treated with HgC12 (100 mg/ml, final culture concentration) in an overlayer of 100 pl/dish after plating of the cells [20]. The dynamic growth of colonies cultured in vitro was detected and followed by counting two dishes twice a week over a period of 3-6 weeks [21]. All colony counting was performed using the Omnicon Fas TI automated colony counter (Milton Roy Inc., Analytical Product Division, Rochester, NY), as previously decribed [22].…”

Effect of alpha‐ and gamma‐Interferon and tumor necrosis factor on colony formation of two human renal tumor xenografts in vitro

“…When tumors reached about 1.5 cm3 in size, the mice were killed; and after small pieces were taken for passage of the tumor, tumor material was suspended in McCoy's wash (Gibco, Paisley, UK). After careful removal of tumor necrosis, tumors were cut into pieces of about 3-4 mm3 and single-cell suspensions were made as described previously [24].…”