Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

Zheng, Jing; Yang, Peng; Dai, Jihong; Yu, Guijuan; Ou, Weihao; Xu, Weiqi; Mai, Kangsen

doi:10.3389/fmars.2020.00198

Cited by 13 publications

(11 citation statements)

References 57 publications

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“…For example, proinflammatory cytokines, such as IL-1β, IL-8, and IFN-γ, were linked to reduced tight junction protein gene expression. 67 TNF-α is known to play dual roles in inflamed microenvironments of IECs, leading to barrier defects on the one hand, yet contributing to wound healing on the other hand. 68 Mechanistically, we could also describe how the response on tight junction proteins elicited by AOH and ATX-II ( Figure 8 ) could be modulated by co-incubation with CH-22, indicating an involvement of the AhR pathway in these effects.…”

Section: Discussionmentioning

confidence: 99%

“…In fact, bidirectional effectivity on epithelial barrier function was previously reported for both cytokines investigated in this study, among others. For example, proinflammatory cytokines, such as IL-1β, IL-8, and IFN-γ, were linked to reduced tight junction protein gene expression . TNF-α is known to play dual roles in inflamed microenvironments of IECs, leading to barrier defects on the one hand, yet contributing to wound healing on the other hand .…”

Section: Discussionmentioning

confidence: 99%

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Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells

Groestlinger

Spindler

Pahlke

et al. 2022

Chem. Res. Toxicol.

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After ingestion of food commodities, the gastrointestinal tract (GIT) poses the first barrier against xenobiotics and pathogens. Therefore, it is regularly confronted with external stressors potentially affecting the inflammatory response and the epithelial barrier. Alternaria mycotoxins such as alternariol (AOH) and altertoxin II (ATX-II) are frequently occurring food and feed contaminants that are described for their immunomodulatory capacities. Hence, this study aimed at exploring the effect of AOH and ATX-II as single compounds or binary mixtures on the immune response and epithelial homeostasis in noncancerous colon epithelial cells HCEC-1CT. Both toxins suppressed mRNA levels of proinflammatory mediators interleukin-8 (IL-8), tumor necrosis factor α (TNF-α), and secretion of IL-8, as well as mRNA levels of the matrix metallopeptidase 2 (MMP-2). Binary combinations of AOH and ATX-II reduced the response of the single toxins. Additionally, AOH and ATX-II modified immunolocalization of transmembrane proteins such as integrin β1, zona occludens 1 (ZO-1), claudin 4 (Cldn 4), and occludin (Ocln), which support colonic tissue homeostasis and intestinal barrier function. Moreover, the cellular distribution of ZO-1 was affected by ATX-II. Mechanistically, these effects could be traced back to the involvement of several transcription factors. AOH activated the nuclear translocation of the aryl hydrocarbon receptor (AhR) and the nuclear factor erythroid 2-related factor 2 (Nrf2), governing cell metabolic competence and structural integrity. This was accompanied by altered distribution of the NF-κB p65 protein, an important regulator of inflammatory response. ATX-II also induced AhR and Nrf2 translocation, albeit failing to substantiate the effect of AOH on the colonic epithelium. Hence, both toxins coherently repress the intestinal immune response on the cytokine transcriptional and protein levels. Furthermore, both mycotoxins affected the colonic epithelial integrity by altering the cell architecture.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells

Groestlinger

Spindler

Pahlke

et al. 2022

Chem. Res. Toxicol.

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“…D-lactic acid is a metabolic product of bacteria mainly found in the intestinal mucosa ( Liu et al., 2020b ). A complete intestinal mucosal barrier can prevent DAO and D-LA from entering the blood circulation ( Zheng et al., 2020 ). Therefore, the intestinal barrier function can be assessed indirectly by assessing plasma DAO activity and D-LA concentration ( Wang et al., 2020 ).…”

Section: Discussionmentioning

confidence: 99%

The combined impact of xylo-oligosaccharides and gamma-irradiated Astragalus polysaccharides on growth performance and intestinal mucosal barrier function of broilers

Wang

Xing

et al. 2021

Poultry Science

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This study was conducted to investigate the individual and combined effects of xylo-oligosaccharides ( XOS ) and gamma-irradiated Astragalus polysaccharides ( IAPS ) on the growth performance and intestinal mucosal barrier function of broiler chickens. A total of 240 1-day-old Ross-308 chicks were allocated into 5 groups for 21 d: control group (basal diet), antibiotic growth promoter ( AGP ) group (basal diet supplemented with 50 mg/kg chlortetracycline), XOS group (basal diet supplemented with 100 mg/kg XOS), IAPS group (basal diet supplemented with 600 mg/kg IAPS), and XOS + IAPS group (basal diet supplemented with 100 mg/kg XOS and 600 mg/kg IAPS). The results showed that birds in the XOS + IAPS group showed higher ADG and lower feed-to-gain ratio than those in the control group ( P < 0.05). The XOS, IAPS, and XOS + IASP treatments significantly increased villus height ( VH ) of all intestine segments, jejunal goblet cell numbers, and VH–to–crypt depth ratio ( VH/CD ) of broilers than those of the control group ( P < 0.05). Birds in the XOS + IAPS group had higher jejunal VH/CD ratio and goblet cell numbers than those from the XOS or IAPS groups ( P < 0.05). In addition, there was a synergy effect between XOS and IAPS on increasing duodenal goblet cell numbers and improving ileal morphology (higher VH and VH/CD ratio) ( P < 0.05). The XOS, IAPS and XOS + IAPS treatments increased the mRNA expression of zonula occludens-1 and occludin of the jejunum as compared with the control group ( P < 0.05). Simultaneously, birds in the XOS + IAPS group showed lower plasma D-lactic acid concentration and higher mRNA expression of claudin-1, claudin-3, and occludin in the jejunum than those in the control or IAPS groups ( P < 0.05). Moreover, there was no significant difference in growth performance, intestinal morphology, and intestinal barrier function of broilers between the AGP and XOS + IAPS groups. In conclusion, the combination of XOS and IAPS had a better potential as chlortetracycline substitute for improving the growth performance, intestinal morphology, and intestinal barrier function of broilers.

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“…However, SM contains some anti-nutritional factors including trypsin inhibitors, antigen proteins, lectins, tannins, saponins and alkaloids ( 1 – 3 ). Previous studies on Atlantic salmon ( Salmo salar ) ( 4 , 5 ), zebrafish ( Danio rerio ) ( 6 ), rice field eel ( Monopterus albus ) ( 7 ), Japanese seabass ( Lateolabrax maculatus ) ( 8 ), largemouth bass ( 9 ) and Mirror carp ( Cyprinus carpio ) ( 10 ) have shown that, excessive use of SM in feed can cause intestinal inflammation and injury, intestinal digestion and absorption dysfunction, and ultimately reduce growth performance ( 11 , 12 ), especially in carnivorous fish.…”

Section: Introductionmentioning

confidence: 99%

Effects of tributyrin and alanyl-glutamine dipeptide on intestinal health of largemouth bass (Micropterus salmoides) fed with high soybean meal diet

et al. 2023

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To investigate the effects of dietary tributyrin (TB) and alanyl-glutamine (AGn) on the intestinal health of largemouth bass (Micropterus salmoides) fed with high-level soybean meal (SM) diet, six isonitrogenous (41.36%) and isolipidic (10.25%) diets were formulated and fed to largemouth bass (initial body weight 25.5 ± 0.5g) for 8 weeks. The two control diets contained 34.8% peanut meal (PM) and 41.3% SM, while the other four experimental diets supplemented TB at 0.1% (TB0.1), 0.2% (TB0.2) and AGn at 1% (AGn1), 2% (AGn2) in SM, respectively. The results showed that there were no significant differences in weight gain, survival rate, and hepatosomatic index among all groups (P>0.05), while feed coefficient rate in AGn1, AGn2 and TB0.2 groups was significantly lower than that in SM group (P< 0.05). Compared with the PM group, the intestinal inflammation of largemouth bass in SM group were obvious, accompanied by the damage of intestinal structure, the decrease of digestive enzyme activity, and the up-regulation of proinflammatory cytokines. Compared with the SM group, the activities of intestinal trypsin, lipase and foregut amylase in TB and AGn groups increased significantly (P<0.05), and the gene expression levels of acetyl-CoA carboxylase (ACC), caspase-3, caspase-8, caspase-9, tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β) were down-regulated, while the gene expression levels of target of rapamycin (TOR) and eIF4E-binding protein (4E-BP) were up-regulated in all experimental groups (P<0.05). It can be concluded that supplementation of 1%-2% AGn and 0.1%-0.2% TB can alleviate enteritis caused by high-level soybean meal, and the recommend level is 2% AGn and 0.2% TB.

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Dynamics of Intestinal Inflammatory Cytokines and Tight Junction Proteins of Turbot (Scophthalmus maximus L.) During the Development and Recovery of Enteritis Induced by Dietary β-Conglycinin

Cited by 13 publications

References 57 publications

Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells

Alternaria alternata Mycotoxins Activate the Aryl Hydrocarbon Receptor and Nrf2-ARE Pathway to Alter the Structure and Immune Response of Colon Epithelial Cells

The combined impact of xylo-oligosaccharides and gamma-irradiated Astragalus polysaccharides on growth performance and intestinal mucosal barrier function of broilers

Effects of tributyrin and alanyl-glutamine dipeptide on intestinal health of largemouth bass (Micropterus salmoides) fed with high soybean meal diet

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