2003
DOI: 10.1002/aic.690490828
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Dynamics of isoelectric precipitation of casein using sulfuric acid

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Cited by 8 publications
(3 citation statements)
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“…Proteins are typically least soluble at the isoelectric point, the pH at which the net charge of the protein is neutral. Lowering the pH of samples to the isoelectric point of casein (4.6) destabilizes casein micelles in milk thereby inducing isoelectric precipitation, allowing for casein elimination ( 27 , 28 ). Palivizumab recovery with this approach was low ( Table 3 ; Experiment 2.2.4).…”
Section: Resultsmentioning
confidence: 99%
“…Proteins are typically least soluble at the isoelectric point, the pH at which the net charge of the protein is neutral. Lowering the pH of samples to the isoelectric point of casein (4.6) destabilizes casein micelles in milk thereby inducing isoelectric precipitation, allowing for casein elimination ( 27 , 28 ). Palivizumab recovery with this approach was low ( Table 3 ; Experiment 2.2.4).…”
Section: Resultsmentioning
confidence: 99%
“…It is also possible to use a simplified method to estimate the mixing time in the microreactor by using a micromixing equation32: where t micro denotes the mixing time in the microscale system in seconds; λ K denotes the Kolmogorov length scale in meters; D denotes the diffusivity in the liquid phase in m 2 s −1 ; ν denotes the kinematic viscosity in m 2 s −1 ; ε denotes the energy dissipation rate in W kg −1 .…”
Section: Resultsmentioning
confidence: 99%
“…High selectivity in a precipitation process is particularly desirable since early purification means subsequent high resolution purification steps will be less susceptible to fouling6 and fewer steps may be needed thereby improving the overall yield 7. Precipitation strategies demonstrated to offer selectivity include isoelectric precipitation,8 precipitation by non‐ionic polymers [such as polyethylene glycol (PEG)]9 and divalent or multivalent cations 10–12…”
Section: Introductionmentioning
confidence: 99%