Dynamics of liposomes in the fluid phase

Gupta, Sudipta; Mel, Judith U. De; Schneider, Gerald J.

doi:10.1016/j.cocis.2019.05.003

Cited by 49 publications

(62 citation statements)

References 110 publications

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“… a We used the results of the 0 wt % d -C 18 - d -PEO4-DOPC sample from our previous study. 41 , 50 , 61 For comparison with pure DOPC, we have included κ η for N = 2 and 1 layers. …”

Section: Resultsmentioning

confidence: 99%

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Manipulating Phospholipid Vesicles at the Nanoscale: A Transformation from Unilamellar to Multilamellar by an n-Alkyl-poly(ethylene oxide)

et al. 2021

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We investigated the influence of an n -alkyl-PEO polymer on the structure and dynamics of phospholipid vesicles. Multilayer formation and about a 9% increase in the size in vesicles were observed by cryogenic transmission electron microscopy (cryo-TEM), dynamic light scattering (DLS), and small-angle neutron/X-ray scattering (SANS/SAXS). The results indicate a change in the lamellar structure of the vesicles by a partial disruption caused by polymer chains, which seems to correlate with about a 30% reduction in bending rigidity per unit bilayer, as revealed by neutron spin echo (NSE) spectroscopy. Also, a strong change in lipid tail relaxation was observed. Our results point to opportunities using synthetic polymers to control the structure and dynamics of membranes, with possible applications in technical materials and also in drug and nutraceutical delivery.

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“… a We used the results of the 0 wt % d -C 18 - d -PEO4-DOPC sample from our previous study. 41 , 50 , 61 For comparison with pure DOPC, we have included κ η for N = 2 and 1 layers. …”

Section: Resultsmentioning

confidence: 99%

“… 55 Considering the neutral surface as the interface between the hydrophilic headgroup and the hydrophobic tail ( h = h t ), 56 − 60 we can redefine eq 11 to obtain the bending rigidity of a bilayer from ULV 55 Equation 12 has been successfully used to calculate κ η for ULVs from NSE. 41 , 46 , 55 , 61 …”

Section: Theoretical Backgroundmentioning

confidence: 99%

Manipulating Phospholipid Vesicles at the Nanoscale: A Transformation from Unilamellar to Multilamellar by an n-Alkyl-poly(ethylene oxide)

et al. 2021

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“…6,7,28,37,39 The numerical prefactor of 0.0069 seems to be the most up to date value as discussed in our recent review. 27 In Table 3 . 28 This determines the effective diffusion for the membrane undulation.…”

Section: Contribution Of Height-height Correlation Zilman-granek Modelmentioning

confidence: 99%

“…25,26 We compared four different phospholipid samples, DOPC (1,2-dioleoyl-sn-glycero-3-phosphocholine), DSPC (1,2-distearoyl-sn-glycero-3-phosphocholine), DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and SoyPC (L-aphosphatidylcholine), in their fluid phases. 27 By a detailed calculation of the time evolution of 〈Δ ( ) % 〉, we obtained three distinct power-laws in the time range of the NSE experiment. We found t 1 at longer Fourier times, followed by t 0.66 and t 0.26 ( < 5 ns), at intermediate and shorter Fourier times, respectively.…”

Section: Introductionmentioning

confidence: 99%

Modeling the dynamics of phospholipids in the fluid phase of liposomes

Gupta

Schneider

2020

Soft Matter

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We present the derivation of a new model to describe neutron spin echo spectroscopy and quasielastic neutron scattering data on liposomes. We compare the new model with existing approaches and benchmark it with experimental data. The analysis indicates the importance of including all major contributions into modeling of the intermediate scattering function. Simultaneous analysis of the experimental data on lipids with full contrast and tail contrast matched samples, reveals highly confined lipid tail motion. A comparison of their dynamics demonstrates the statistical independence of tail-motion and height-height correlation of the membrane. A more detailed analysis indicates that lipid tails are subject to relaxations in a potential with cylindrical symmetry, in addition to the undulation and diffusive motion of the liposome. Despite substantial differences in the chemistry of the fatty acid tails, the observation indicates a universal behavior. The analysis of partially deuterated systems confirms the strong contribution of the lipid tail to the intermediate scattering function. Within the time range from 5 to 100 ns, the intermediate scattering function can be described by the height-height correlation function. The existence of the fast-localized tail motion and the contribution of slow translational diffusion of liposomes determines the intermediate scattering function for t < 5 ns and t > 100 ns, respectively. Taking into account the limited

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“…temperature, drugs, among others) without the interference of other physiological effects occuring in living cells. 23,34,35,[37][38][39] Starting from the measurements on liposomes in solution, we best-fitted the QENS curves by using one delta function that relates to the elastic scattering, and two Lorentzians that account for the quasi-elastic dynamics. In previous investigations on POPC bilayers performed in a comparable time-range, Wanderlingh et al have employed a three Lorentzians model, with a broad Lorentzian (HWHM ≈ 2 meV) attributed to the motion of hydrogen with respect to the bounding carbon atom (0.33 ps), and two narrow components related to localized motions, such as lipid chain conformational dynamics (≈ 0.8 meV) and motion of the whole phospholipid molecules (0.05 meV).…”

Section: Results and Discussion A) Effects On Model Membranesmentioning

confidence: 99%

The Effect of an Intramembrane Light-Actuator on the Dynamics of Phospholipids in Model Membranes and Intact Cells

et al. 2020

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The non-covalent intercalation of amphiphilic molecules in the lipid membrane can be exploited to modulate efficiently the physical status of the membrane. Such effects are largely employed in a range of applications, spanning from drug-delivery to therapeutics. In this context, we have very recently developed an intramembrane photo-actuator consisting of an amphiphilic azobenzene molecule, namely ZIAPIN2. The selective photo-isomerization occurring in the lipid bilayer induces a photo-triggered change in the membrane thickness and capacitance, eventually permitting to evoke light-induced neuronal firing both in vitro and in vivo.Here, we present a study on the dynamical perturbation in the lipid membrane caused by ZIAPIN2 and its vehicle solvent, dimethyl sulfoxide. Effects on the dynamics occurring in the picosecond time range and at the molecular level are probed using quasi-elastic neutron scattering. By coupling experiments carried out both on model membranes and intact cells, we found that DMSO leads to a general retardation of the dynamics within a more dynamically ordered landscape, a result that we attribute to the dehydration at the interface. On the other hand, ZIAPIN2 partitioning produces a general softening of the bilayer owing to its interaction with the lipids. These data are in agreement with our recent studies, which indicate that the efficacy of ZIAPIN2 in triggering cellular signalling stems from its ability to mechanically perturb the bilayer as a whole, by forming light-sensitive membrane spanning dimers.

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Dynamics of liposomes in the fluid phase

Cited by 49 publications

References 110 publications

Manipulating Phospholipid Vesicles at the Nanoscale: A Transformation from Unilamellar to Multilamellar by an n-Alkyl-poly(ethylene oxide)

Manipulating Phospholipid Vesicles at the Nanoscale: A Transformation from Unilamellar to Multilamellar by an n-Alkyl-poly(ethylene oxide)

Modeling the dynamics of phospholipids in the fluid phase of liposomes

The Effect of an Intramembrane Light-Actuator on the Dynamics of Phospholipids in Model Membranes and Intact Cells

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