Background
The genetic diversity of malaria parasites contributes to their ability to adapt to environmental changes, develop drug resistance and circumvent the host immune system. This study aimed to analyse the genetic diversity of the
Pfmsp1
and
Pfmsp2
genes in
Plasmodium falciparum
and the
Pvmsp-3α
gene in
Plasmodium vivax
isolates from District Nowshera in Pakistan.
Methods
Blood samples from 124 consenting patients with uncomplicated malaria presenting to different hospitals from the Nowshera district were collected between March and August 2019, representing 28
P. falciparum
and 96
P. vivax
isolates. The genomic DNA extracted from the isolates were subjected to nested PCR and allele-specific analysis.
Pvmsp-3α
amplified fragments were further treated with restriction fragment length polymorphism (RFLP)-based
Hha1
restriction enzyme.
Results
Of the analyzed
P. falciparum,
21 distinct alleles were detected, including 14 alleles for
Pfmsp-1
and 7 alleles for
Pfmsp-2
. The sub-allelic families MAD20 (50%) of
Pfmsp-1
and FC27 (75%) of
Pfmsp-2
were predominant. The multiplicity of infection (MOI) was calculated as 1.4 and 1.2 for
Pfmsp-1
and
Pfmsp
-
2,
respectively, with an overall mean MOI of 1.34. In
P. vivax
, 4 allelic variants,
Pvmsp-
3α types
A, B, C
and
D
, were detected, while RFLP digestion of amplicons, detected 9 sub-allelic variants (
A1-A4
,
B1
,
B2
,
C1
,
C2
and
D1
) at the
Pvmsp-3α
locus.
Conclusion
This first ever report of molecular characterization of
P. falciparum
and
P. vivax
genotypes from District Nowshera, Pakistan reveals moderate to high allelic diversity in parasite population from District Nowshera, Pakistan.