Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant

Yang, Lili; Yuan, Xianjun; Li, Junfeng; Dong, Zhihao; Shao, Tao

doi:10.1016/j.biortech.2018.12.067

Cited by 206 publications

(186 citation statements)

References 33 publications

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“…Although the Shannon indexes were similar between the 30-d and 90-d silage, the PCoA showed variation within the bacterial community. The clear separation between bacterial communities of the ensiling material and alfalfa silage indicated a shift after ensiling, consistent with previous studies (Ni et al, 2017b;Yang et al, 2019). Divisions between the plots representing silage with and without inoculants indicated that the distribution of the bacterial community was shifted by L. plantarum inoculations, which could explain the differences in fermentation quality (Ni et al, 2018).…”

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Sisupporting

confidence: 89%

“…Inoculation with L. plantarum had a positive effect on fermentation properties in terms of lower pH and higher LA accumulation. Similar effects were also reported by Wang et al (2019) and Yang et al (2019), in which LA fermentation was accelerated by L. plantarum inoculants in Moringa oleifera leaves and in alfalfa silage, respectively. The LPII exhibited more abundant LA fermentation than LPI.…”

Section: Effects Of L Plantarum On Fermentation Properties Of Alfalfsupporting

confidence: 86%

“…Thus, L. plantarum is the most commonly used bacterial inoculant in forage ensiling studies (Oliveira et al, 2017). During ensiling, L. plantarum enhances LA fermentation and survives and adapts to the silage environment (Guo et al, 2018;Ogunade et al, 2018;Yang et al, 2019). However, the fermentation quality of the silage varies owing to different factors, such as BC, water activity, and presence of epiphytic microbes.…”

Section: Introductionmentioning

confidence: 99%

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Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

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BackgroundThe objective of this study was to investigate the mechanism of Lactobacillus plantarum (L. plantarum) involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. ResultsHigh-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two L. plantarum additives (LPI and LPII). The pH and fermentation products of silage were investigated, and the bacterial community compositions were analyzed. The L. plantarum inoculants significantly enhanced the lactic acid fermentation in terms of promotions in pH decline, lactic acid accumulation, and Lactobacillus abundance for both periods. At 90 d, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of L. plantarum significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage (P < 0.05). Conclusions Inoculation with L. plantarum improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed decomposition of alfalfa silage under adverse ensiling conditions.

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Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Sisupporting

confidence: 89%

Section: Effects Of L Plantarum On Fermentation Properties Of Alfalfsupporting

confidence: 86%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

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“…reported that Lactobacillus had strong positive relationships with inoculant groups as compared to control. According to Yang et al . the better relationships among the fermentation period and bacterial population during the ensiling process of alfalfa silage, heatmap spearman correlation on the genus ( Lactobacillus ) level was negatively correlated with pH, while positively correlated with LA, while the Bacillus , Enterobacter , Leuconostoc , Pediococcus , and Weissella showed positive relationships with pH and negative relationships with LA.…”

Section: Discussionmentioning

confidence: 99%

Effect of endophytic Bacillus megaterium colonization on structure strengthening, microbial community, chemical composition and stabilization properties of Hybrid Pennisetum

et al. 2019

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BACKGROUND: This study was conducted to analyze the effects of endophytic Bacillus megaterium (BM 18-2) colonization on structure strengthening, microbial community, chemical composition and stabilization properties of Hybrid Pennisetum. RESULTS: The BM 18-2 had successfully colonized in the interior tissues in both leaf and stem of Hybrid Pennisetum. During ensiling, the levels of pH, acetic acid (AA), butyric acid (BA), propionic acid (PA), and the population of yeast and aerobic bacteria were significantly (P > 0.05) lower, while lactic acid bacteria (LAB) and lactic acid (LA) were significantly (P < 0.001) higher with the steps forward of ensiling in with BM 18-2 as compared to without BM 18-2 colonized of Hybrid Pennisetum. During the different ensiling days, at days 3, 6, 15, and 30, the genus Brevundimonas, Klebsiella, Lactococcus, Weissella, Enterobacter, Serratia, etc. population were significantly decreased, while genus Pediococcus acidilactici and Lactobacillus plantarum were significantly influenced in treated groups as compared to control. The genus Lactobacillus and Pediococcus were positively correlated with treatment groups.CONCLUSIONS: It is concluded that the endophytic bacteria strain BM 18-2 significantly promoted growth characteristics and biomass yield before ensiling and after ensiling inoculated with or without Lactobacillus plantarum could improve the distinct changes of the undesirable microbial diversity, chemical composition, and stabilization properties in with BM 18-2 as compared to without BM 18-2 colonized Hybrid Pennisetum.

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“…Moreover, N. cadamba leaves (NCL) contain high content polyphenols like tannins (hydrolysable tannins: 44.2 g kg −1 DM, condensed tannins: 69.6 g kg −1 DM, He et al , ), which could restrict proteolysis in ensiled forage (Guo et al , ). The extract of NCL had significant antibacterial activities on undesirable bacteria like Staphylococcus , Bacillus , Escherichia (Khandelwal et al , ; Pandey and Negi, ) , which are also frequently detected in silage (Yang et al , ; Zhang et al , ).…”

Section: Introductionmentioning

confidence: 99%

Effects of mixing Neolamarckia cadamba leaves on fermentation quality, microbial community of high moisture alfalfa and stylo silage

Wang

Xing

et al. 2019

Microbial Biotechnology

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Summary Neolamarckia cadamba is not only a fodder of high nutritional value, but also a source of natural antimicrobial agent. The silage quality of high moisture alfalfa and stylo with or without N. cadamba leaves (NCL) was investigated, and microbial community after ensiling was analysed. Results showed that the silage samples with NCL have lower pH (4.32 versus 4.88, 4.26 versus 4.71 in alfalfa and stylo silage, respectively), ammonia‐N content (67.5 versus 146, 42.2 versus 95.1 g kg−1 total N) and higher lactic acid (13.3 versus 10.4, 17.3 versus 13.6 g kg−1 dry matter), true protein N (592 versus 287, 815 versus 589 g kg−1 total N). The addition of NCL also influenced the bacterial community distribution. The relative abundance of Clostridium and Enterobacter decreased, whereas Lactobacillus abundance increased when NCL was added. In conclusion, NCL could inhibit undesirable microorganisms in high moisture alfalfa and stylo silage. Mixing with NCL could be a feasible way to improve the quality of silage.

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Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant

Cited by 206 publications

References 33 publications

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Effect of endophytic Bacillus megaterium colonization on structure strengthening, microbial community, chemical composition and stabilization properties of Hybrid Pennisetum

Effects of mixing Neolamarckia cadamba leaves on fermentation quality, microbial community of high moisture alfalfa and stylo silage

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