Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar

Zheng, Mingli; Niu, Dongze; Jiang, Dedai; Zuo, Shengpeng; Xu, Chuncheng

doi:10.1111/jam.13456

Cited by 120 publications

(127 citation statements)

References 48 publications

(71 reference statements)

Supporting

Mentioning

109

Contrasting

Unclassified

Order By: Relevance

“…Although richness of bacterial community decreased compared with the ensiling material at the early stage of ensiling, the richness ourished at 90 d. Similar trends were also reported by Zheng et al (2017) in direct-cut alfalfa silage with and without LAB inoculant and sugar and by Keshri et al (2018) in untreated corn silage, in which richness of the bacterial community decreased at the early stage of ensiling compared with the alfalfa material, and later increased during the ensiling process. Consistent with the study of Ni (2017a), diversity of the bacterial community decreased after ensiling.…”

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Sisupporting

confidence: 80%

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

View full text Add to dashboard Cite

BackgroundThe objective of this study was to investigate the mechanism of Lactobacillus plantarum (L. plantarum) involved in improving fermentation quality of naturally ensiled alfalfa under poor conditions. ResultsHigh-moisture wilted alfalfa was ensiled without inoculants (CK) or with inoculation of two L. plantarum additives (LPI and LPII). The pH and fermentation products of silage were investigated, and the bacterial community compositions were analyzed. The L. plantarum inoculants significantly enhanced the lactic acid fermentation in terms of promotions in pH decline, lactic acid accumulation, and Lactobacillus abundance for both periods. At 90 d, silage in CK exhibited a high pH, a loss in dry matter, and a high concentration of ammoniacal nitrogen. The inoculations of L. plantarum significantly inhibited the growth of Clostridia, and reduced ammoniacal nitrogen concentration in silage (P < 0.05). Conclusions Inoculation with L. plantarum improved the fermentation quality of alfalfa silage and inhibited the growth of spoilage microorganisms, and further delayed decomposition of alfalfa silage under adverse ensiling conditions.

show abstract

Section: Effects Of L Plantarum On Bacterial Community Of Alfalfa Sisupporting

confidence: 80%

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Yang¹,

Wang²,

Zhao³

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Then, three PCR products were mixed in equi-density ratios. Finally, mixture was purified for DNA concentration and sequencing (Li et al 2015;Zheng et al 2017). Therefore, the data of high-throughput sequencing were not able to be further statistically analyzed.…”

Section: Analysis Of Microbial Diversity Through High-throughput Sequmentioning

confidence: 99%

Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

Zhang

et al. 2018

Journal of Integrative Agriculture

View full text Add to dashboard Cite

This study assessed the effects of lactic acid bacteria (LAB), cellulase, cellulase-producing Bacillus pumilus and their combinations on the fermentation characteristics, chemical composition, bacterial community and in vitro digestibility of alfalfa silage. A completely randomized design involving a 8 (silage additives)×3 or 2 (silage days) factorial arrangement of treatments was adopted in the present study. The 8 silage additive treatments were: untreated alfalfa (control); two commercial additives (GFJ and Chikuso-1); an originally selected LAB (Lactobacillus plantarum a214) isolated from alfalfa silage; a cellulase-producing Bacillus (CB) isolated from fresh alfalfa; cellulase (C); and the combined additives (a214+C and a214+CB). Silage fermentation characteristics, chemical composition and microorganism populations were analysed after 30, 60 and 65 days (60 days followed by exposure to air for five additional days). In vitro digestibility was analysed for 30 and 60 days. Compared with the other treatments, selected LAB a214, a214 combined with either C or CB, and Chikuso-1 had the decreased (P<0.001) pH and increased (P<0.001) lactic acid concentrations during the ensiling process, and there were no differences (P>0.05) among them. Fiber degradation was not significant (P≥0.054) in any C or CB treatments. The a214 treatment showed the highest (P=0.009) in vitro digestibility of dry matter (595.0 g kg-1 DM) after ensiling and the highest abundance of Lactobacillus (69.42 and 79.81%, respectively) on days 60 and 65, compared to all of other treatments. Overall, the silage quality of alfalfa was improved with the addition of a214, which indicates its potential as an alfalfa silage inoculant.

show abstract

“…Extensive studies have been conducted to disclose the most abundant microbial community in the chicken GIT by using culture-based approach, DNA fingerprinting method and polymerase chain reaction (PCR)-based sequencing (Xiao et al 2017). Although these studies have expanded our knowledge of the intestinal microbiota, they only identified a few of the most abundant operational taxonomic units (OTUs) present, because of their poor limits of detection (Zheng et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Growth performance, carcass characteristics, meat and egg quality, and intestinal microbiota in Beijing-you chicken on diets with inclusion of fresh chicory forage

Zheng

Mao

Tian

et al. 2019

Italian Journal of Animal Science

View full text Add to dashboard Cite

The aim of this study was to evaluate the effects of fresh chicory forage in chicken diets on growth performance, carcass characteristics, meat and egg quality, as well as intestinal microbiota. A total of 600 healthy 16 weeks female Beijing-you chickens were randomly assigned to four dietary treatments containing 0%, 5%, 8% and 10% chicory forage with six replicate pens and 25 chickens in each replicate. Chickens were raised in a free-range system for 13 weeks after seven days of adaptation. High-throughput sequencing was used to characterise the microbiota in three different intestinal sections (duodenum, ileum and caecum). The results showed that dietary supplementation of chicory forage increased (p < .05) egg weight, yolk colour, total amino acids and delicious amino acids in muscles, and decreased (p < .05) abdominal fat yield compared to the control. However, increased (p < .05) average daily feed intake, increased (p < .05) feed conversion ratio, increased (p < .05) mortality and decreased (p < .05) eggshell strength were observed in chickens fed chicory forage than in those fed a basal diet. Dietary supplementation of chicory forage stimulated the proliferation of the Lactobacillus in ileum and the Bacteroides in caecum, and depressed pathogenic microbes including the Rikenella, thereby further improved intestinal health and nutrient utilisation. It was concluded that 8% chicory forage could be considered as a beneficial feed additive in Beijing-you chicken diets. In addition to the good nutritional profile, medicinal properties were associated with the beneficial effects of chicory forage on chickens. HIGHLIGHTS Dietary supplementation of chicory forage improved muscle nutritional value and flavour. Dietary supplementation of chicory forage affected egg quality. Dietary supplementation of chicory forage regulated the intestinal microbiota. ARTICLE HISTORY

show abstract

Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar

Abstract: High-throughput sequencing based on a novel Clostridia-specific primer set proved a potentially useful tool to study the clostridial community dynamics, and could aid to elucidate the mechanism by which the clostridial fermentation develops during the ensiling of alfalfa.

Cited by 120 publications

References 48 publications

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Lactobacillus plantarum inoculation delays decomposition of alfalfa silage under adverse ensiling conditions by regulating bacterial community composition

Influence of lactic acid bacteria, cellulase, cellulase-producing Bacillus pumilus and their combinations on alfalfa silage quality

Growth performance, carcass characteristics, meat and egg quality, and intestinal microbiota in Beijing-you chicken on diets with inclusion of fresh chicory forage

Contact Info

Product

Resources

About