Dynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR

Srivastava, Ankita; Choi, Gang-Guk; Ahn, Chi‐Yong; Oh, Hee‐Mock; Ravi, A.; Asthana, Ravi Kumar

doi:10.1016/j.watres.2011.11.056

Cited by 52 publications

(29 citation statements)

References 42 publications

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“…3A and B). The dominance of MC-RR at the two studied sites is in accordance with reports of Prakash et al (2009) andSrivastava et al (2012). The levels of microcystins at all sampling points at both sites (Fig.…”

Section: Discussionsupporting

confidence: 90%

“…Analyses for microcystins were performed by the LC-MS method described by Srivastava et al (2012). Chromatographic separation was achieved using Thermo Accela w a t e r r e s e a r c h 6 9 ( 2 0 1 5 ) 1 3 1 e1 4 2 UHPLC system (Thermo Electron Corp) coupled with Linear Ion Trap Quadrupole LC-MS Mass spectrometer 4000 Q TRAP AB Sciex instrument (ABS), consisting of a quaternary pump connected to an online degasser, autosampler and photodiode array detector (PDA).…”

Section: Microcystin Analysismentioning

confidence: 99%

“…There are reports of microcystin contamination (Prakash et al, 2009;Srivastava et al, 2012) and presence of MCþ cyanobacteria in several of the Varanasi ponds (Kumar et al, 2011). Microcystis spp.…”

Section: Introductionmentioning

confidence: 99%

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Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds

et al. 2015

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Section: Discussionsupporting

confidence: 90%

Section: Microcystin Analysismentioning

confidence: 99%

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Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds

et al. 2015

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“…In order to quantify the potentially MC-producing genotypes, we used the generalist primers developed by Hisbergues et al (2003) (Table 1) and used for real-time quantitative PCR (qPCR) by Srivastava et al (2012). These primers amplify a fragment from the mcyA gene in most MC-producing genera (Anabaena, Microcystis, Planktothrix, Nostoc.…”

Section: Quantification Of Microcystin Synthesis Genes (Mcya)mentioning

confidence: 99%

Co-occurrence of microcystin and anatoxin-a in the freshwater lake Aydat (France): Analytical and molecular approaches during a three-year survey

et al. 2015

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“…A range of environmental factors, including nitrogen and phosphorus concentrations (Schindler, 2012;Srivastava et al, 2012;Chaffin and Bridgeman, 2014; Van de Waal et al, 2014), TN : TP ratio (Smith, 1983;Wang et al, 2010b; Van de Waal et al, 2014), temperature (Davis et al, 2009;Rolland et al, 2013), salinity (Tonk et al, 2007), and iron concentration (Ame and Wunderlin, 2005;Nagai et al, 2007;Wang et al, 2010a) have been shown to have pronounced effects on cyanobacterial biomass, cyanobacterial dominance and microcystin production. Nevertheless, the results between studies differ, and there is no clear understanding of the roles of these environmental factors as the triggers of cyanobacterial bloom development and microcystin production.…”

Section: S C Sinang Et Al: Site-specific Environmental Triggers Ofmentioning

confidence: 99%

Local nutrient regimes determine site-specific environmental triggers of cyanobacterial and microcystin variability in urban lakes

Sinang

Reichwaldt

Ghadouani

2015

Hydrol. Earth Syst. Sci.

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Abstract. Toxic cyanobacterial blooms in urban lakes present serious health hazards to humans and animals and require effective management strategies. Managing such blooms requires a sufficient understanding of the controlling environmental factors. A range of them has been proposed in the literature as potential triggers for cyanobacterial biomass development and cyanotoxin (e.g. microcystin) production in freshwater systems. However, the environmental triggers of cyanobacteria and microcystin variability remain a subject of debate due to contrasting findings. This issue has raised the question of whether the relevance of environmental triggers may depend on site-specific combinations of environmental factors. In this study, we investigated the site-specificity of environmental triggers for cyanobacterial bloom and microcystin dynamics in three urban lakes in Western Australia. Our study suggests that cyanobacterial biomass, cyanobacterial dominance and cyanobacterial microcystin content variability were significantly correlated to phosphorus and iron concentrations. However, the correlations were different between lakes, thus suggesting a sitespecific effect of these environmental factors. The discrepancies in the correlations could be explained by differences in local nutrient concentration. For instance, we found no correlation between cyanobacterial fraction and total phosphorous (TP) in the lake with the highest TP concentration, while correlations were significant and negative in the other two lakes. In addition, our study indicates that the difference of the correlation between total iron (TFe) and the cyanobacterial fraction between lakes might have been a consequence of differences in the cyanobacterial community structure, specifically the presence or absence of nitrogen-fixing species. In conclusion, our study suggests that identification of significant environmental factors under site-specific conditions is an important strategy to enhance successful outcomes in cyanobacterial bloom control measures.

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Dynamics of microcystin production and quantification of potentially toxigenic Microcystis sp. using real-time PCR

Cited by 52 publications

References 42 publications

Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds

Temporal variations in microcystin-producing cells and microcystin concentrations in two fresh water ponds

Co-occurrence of microcystin and anatoxin-a in the freshwater lake Aydat (France): Analytical and molecular approaches during a three-year survey

Local nutrient regimes determine site-specific environmental triggers of cyanobacterial and microcystin variability in urban lakes

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