Dynamics of mortality of the migratory locust under synchronous infection with entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae) and bacteria Pseudomonas sp.

Lednev, G. R.; Kryukov, V. Yu.; Khodyrev, V. P.; Levchenko, M. A.; Duisembekov, B. A.; Sagitov, A. O.; Глупов, В. В.

doi:10.1134/s1995425508020069

Cited by 12 publications

(7 citation statements)

References 2 publications

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“…In particular, Wei and coworkers 8 showed that topical infection with Beauveria bassiana reduced the taxonomical diversity of the mosquito gut microbiota by decreasing its due to the mass proliferation of Serratia marcescens , which in turn increases susceptibility to the fungus. An additive effect between species of the gut bacteria Pseudomonas and entomopathogenic ascomycetes was demonstrated in locusts 19 . Moreover, in many cases, fungal infections lead to the bacterial decomposition of the killed insects.…”

Section: Introductionmentioning

confidence: 97%

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Polenogova

Kabilov

Tyurin

et al. 2019

Sci Rep

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Gut bacteria influence the development of different pathologies caused by bacteria, fungi and parasitoids in insects. Wax moth larvae became more susceptible to fungal infections after envenomation by the ectoparasitoid Habrobracon hebetor . In addition, spontaneous bacterioses occurred more often in envenomated larvae. We analyzed alterations in the midgut microbiota and immunity of the wax moth in response to H. hebetor envenomation and topical fungal infection ( Beauveria bassiana ) alone or in combination using 16S rRNA sequencing, an analysis of cultivable bacteria and a qPCR analysis of immunity- and stress-related genes. Envenomation led to a predominance shift from enterococci to enterobacteria, an increase in CFUs and the upregulation of AMPs in wax moth midguts. Furthermore, mycosis nonsignificantly increased the abundance of enterobacteria and the expression of AMPs in the midgut. Combined treatment led to a significant increase in the abundance of Serratia and a greater upregulation of gloverin. The oral administration of predominant bacteria ( Enterococcus faecalis, Enterobacter sp. and Serratia marcescens ) to wax moth larvae synergistically increased fungal susceptibility. Thus, the activation of midgut immunity might prevent the bacterial decomposition of envenomated larvae, thus permitting the development of fungal infections. Moreover, changes in the midgut bacterial community may promote fungal killing.

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Section: Introductionmentioning

confidence: 97%

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Polenogova

Kabilov

Tyurin

et al. 2019

Sci Rep

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“…The mortality rate of L. migratoria infected with Pseudomonas sp. was approximately (30-50%) within 3 to 7 days after treatment (Lednev et al, 2008). P. entomophila exhibited a unique characterization which demonstrated specific pathogenic properties to dipteran, lepidopteran, and coleopteran insects (Vodovar et al 2005;Vallet-Gely et al 2010).…”

Section: Molecular Identification Of the Most Bioactive Bacterial Isomentioning

confidence: 99%

“…Insecticidal characteristics of some Pseudomonas spp. such as P. entomophila, P. fluorescens, P. Aeruginosa, and P. chlororaphis have been investigated on coleopteran, dipteran, lepidopteran, and orthopteran pests (Osborn et al, 2002, Vodovar et al 2005, Lednev et al, 2008and Vallet-Gely et al 2010. A unique sepsis characterization resulted from infected locusts with Pseudomonas sp.…”

Section: Molecular Identification Of the Most Bioactive Bacterial Isomentioning

confidence: 99%

Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forskål (Orthoptera: Acrididae)

Mashtoly

El-Zemaity

Abolmaaty

et al. 2019

Egypt J Biol Pest Control

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Thirty bacterial isolates were isolated from the gut contents of diseased/dead locust. Their pathogenicity was tested against 4th instar nymphs of desert locust, Schistocerca gregaria Forskål (Orthoptera: Acrididae). Two isolates, designated DL2 and DL6, out of thirty showed the highest insecticidal activities against locust nymphs in preliminary bracketing. They were bioassayed via leaf dip and per os techniques and toxicity was determined using SAS program. The insecticidal activity of DL6 was more than DL2, whereas LC 50 's values of 35 × 10 6 and 13 × 10 6 cfu's/ml were determined for DL2 and DL6, respectively, after 48 h of leaf-dip treatment. However, LD 50 's value of 53 × 10 6 and 26 × 10 6 cfu's/ml was determined for DL2 and DL6, respectively, after 24 h of per os treatment. The relative potencies of DL6 to DL2 were (2.6 and 2.03) folds in leaf-dip and per os treatments, respectively. Biochemical characterization was conducted, using GEN III MicroPlate™ Biolog identification system and confirmed with molecular identification via 16S rDNA gene sequencing. Nucleotide sequencing of each was submitted to a gene bank and an accession number was generated for each isolate. Obtained bacterial strains DL2 and DL6 were identified as Bacillus weihenstephanensis (KY630645) and Pseudomonas sp. (KY630649), with a similarity of 100 and 75% to B. weihenstephanensis strain PHCDB9 (NR_024697) and Pseudomonas sp. strain DSM11821 (KF417541), respectively. The tested strains proved their potential to be bio-pesticide agents involved in controlling desert locust nymphs.

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“…LT 50 of nymphs treated with two pathogens was shortened to three days compared with 13-17 days for the treatment with B. bassiana and 3-7 days for the treatment with Pseudomonas. Furthermore, an analysis of the dead insects showed that both pathogens could coexist in the locust (Lednev et al 2008). Synergistic effect was also achieved with the combined application of entomopathogenic fungi and nematodes (Barbercheck andKaya 1991, Shulte et al 2009).…”

Section: Multitrophic Interactionsmentioning

confidence: 99%

The parasite-host system as exemplified by the interactions between entomopathogenic fungi and insects

Augustyniuk-Kram

2011

seb

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Dynamics of mortality of the migratory locust under synchronous infection with entomopathogenic fungi (Beauveria bassiana, Metarhizium anisopliae) and bacteria Pseudomonas sp.

Cited by 12 publications

References 2 publications

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Parasitoid envenomation alters the Galleria mellonella midgut microbiota and immunity, thereby promoting fungal infection

Phylogenetic characteristics of novel Bacillus weihenstephanensis and Pseudomonas sp. to desert locust, Schistocerca gregaria Forskål (Orthoptera: Acrididae)

The parasite-host system as exemplified by the interactions between entomopathogenic fungi and insects

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