Dynamics of motile-sperm subpopulation structure in boar ejaculates subjected to “in vitro” capacitation and further “in vitro” acrosome reaction

Ramió, Laura; Rivera, MM; Ramírez-Reveco, Alfredo; Concha, Ilona I.; Peña, Alejandro; Rigau, Teresa; Rodríguez-Gil, Joan E.

doi:10.1016/j.theriogenology.2007.10.021

Cited by 59 publications

(81 citation statements)

References 25 publications

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“…Whereas subpopulations containing progressive sperm reacted with significant increases in velocity and linearity, those containing nonprogressive spermatozoa did not practically modify their motility parameters. Moreover, the subpopulation with the most rapid and progressive sperm seemed to be the most prone to undergo the acrosome reaction after progesterone treatment [32].…”

Section: Discussionmentioning

confidence: 97%

“…Therefore, the sperm reservoir within the oviductal isthmus would be expected to be colonized preferably by spermatozoa selected from subpopulations showing forward progressive motility in fresh ejaculates. In this sense, a recent study [32] has shown that boar spermatozoa from separate motile subpopulations responded differently to in vitro capacitation. Whereas subpopulations containing progressive sperm reacted with significant increases in velocity and linearity, those containing nonprogressive spermatozoa did not practically modify their motility parameters.…”

Section: Discussionmentioning

confidence: 97%

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Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

et al. 2009

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The aim of this study was to identify different motile sperm subpopulations in ejaculates from an autochthonous bull breed (Bos taurus) and to determine possible modifications in these subpopulations resulting from cryopreservation. Ejaculates were collected and cryopreserved following a conventional protocol. The overall sperm motility and the kinematic parameters of individual spermatozoa were evaluated in fresh ejaculates, after 4 h at 5 8C, and at 0 and 2 h postthaw. A multivariate clustering procedure separated 23,585 motile spermatozoa into four subpopulations: Subpopulation 1 showed medium velocity (VCL: 99.4 AE 17.8 mm/sec) and high progressiveness (LIN: 65.1 AE 14.0%); Subpopulation 2 included spermatozoa with high velocity (VCL: 148.7 AE 25.6 mm/sec) but a nonprogressive trajectory (LIN: 33.1 AE 10.5%); Subpopulation 3 represented slowly motile (VCL: 58.3 AE 24.3 mm/sec) and nonprogressive sperm (LIN: 39.6 AE 18.3%); and Subpopulation 4 included very rapid (VCL: 152.8 AE 25.7 mm/sec) and highly progressive sperm (LIN: 70.9 AE 13.7%). Subpopulation 4 was present in the greatest quantity in fresh ejaculates (36%), but after cooling, it significantly decreased (21%) concomitantly with an increase (P < 0.001) in Subpopulation 2 (from 21% in fresh to 34% in postcooled semen). After freezing and thawing, the overall sperm motility was reduced, mainly due to Subpopulation 2 decreasing from 34% after cooling to 14% after thawing. Differences among bulls in the frequency distribution of spermatozoa within subpopulations were evidenced after thawing by different proportions of spermatozoa in Subpopulations 2 and 4. The current results indicate that a structure of four sperm subpopulations may be a common characteristic of bovine ejaculates and that the cooling phase of cryopreservation seems to be the determinant of postthaw semen quality. #

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

et al. 2009

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“…High individual variation of the ejaculate among boars [18] and among sperm subpopulations [1,10,20], as well as the composition of the spermatozoa and even the season [2], prevent a predictive analysis of resistance to freezing for a sample of swine semen. These variations make it difficult to establish a standardized freezing protocol.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose

et al. 2015

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In swine spermatozoa, the damage caused by cryopreservation is more severe than other species, provoking reduced potential for fertilization. Adjustments in the freezing extender composition may be an important alternative to increase its efficiency. The objective of this study was to test the efficiency of different cryoprotectant solutions during cryopreservation of swine semen with a controlled cooling curve. Three cryoprotectant solutions (5% dimethylformamide, 3% glycerol and the combination of these two cryoprotectants) were used in association with three base media (powdered coconut water, lactose and trehalose), constituting nine different treatments. The semen was frozen using a controlled-rate freezer (TK-3000). After thawing, semen was evaluated for total sperm motility, vigor, morphology, plasma membrane integrity and acrosome integrity. Cryopreservation with the controlled curve using an automated system showed satisfactory results, guaranteeing practicality and repeatability for the process of freezing swine sperm. With this curve, the solutions of lactose, trehalose and powdered coconut water associated with glycerol, as well as the solution of coconut water containing dimethylformamide, presented higher quality of sperm compared to the other solutions. Powdered coconut water associated with dimethylformamide appears as a new solution for swine sperm cryopreservation. The freezing controlled curve used in this study allowed standardization of the cryopreservation technique.

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“…Capacitated boar sperm respond in a complex manner to a high concentration of progesterone but are clearly sensitive to this hormone and remain responsive over time [24]. The threshold sensitivity of boar sperm to progesterone has not been established; however in this study we show that 100nM progesterone significantly increases the rate of penetration in both non-capacitated and capacitated cells.…”

Section: Discussionmentioning

confidence: 46%

“…However, high (micromolar) concentrations have been reported to increase motility [24] and intracellular calcium levels [14] in capacitated cells. Generally, computer assisted sperm analysis (CASA) is employed to examine sperm motility.…”

Section: Introductionmentioning

confidence: 99%

Progesterone significantly enhances the mobility of boar spermatozoa

Campbell¹,

Savage²,

Madamidola³

et al. 2013

Bidiscovery

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Progesterone released from the cumulus cells of the oocyte causes a number of physiological responses in human sperm cells including hyperactivation, acrosome reaction and chemotaxis. We employed a validated sperm mobility assay, which involves measuring the ability of sperm to penetrate an inert cell separation solution over time, to assess the ability of progesterone to enhance the mobility of boar spermatozoa. Cells maximally penetrate the solution over 50 minutes. 100nM progesterone significantly (P = 0.01) increased the mobility of non-capacitated sperm cells causing a doubling in the rate at which the cells penetrated through the cell separation solution (control half maximal penetration rate [Km] = 18.0±2.2; +100nM progesterone Km = 8.8±0.8min). Similarly, capacitated cells penetrated at a rate (Km = 19.2±3.0 min) not significantly different from non-capacitated cells and 100nM progesterone also significantly increased the rate of penetration of capacitated cells (Km = 9.5±1.0 min, P<0.05). The T-type voltage gated calcium channel blocker mibefradil (30mM) significantly inhibited both the control and progesterone enhanced mobility in non-capacitated and capacitated sperm. Only capacitated cells showed a significant increase in the acrosome reaction in response to 100nM progesterone (control non-reacted = 75±4%, +100nM progesterone nonreacted = 47±10%). Western blot analysis confirmed that there was an increase in the total protein tyrosine phosphorylation levels in capacitated cells. In conclusion, we have demonstrated that 100nM progesterone accelerates the mobility of boar sperm cells through an inert cell separation solution in an extracellular calcium dependent manner.

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Dynamics of motile-sperm subpopulation structure in boar ejaculates subjected to “in vitro” capacitation and further “in vitro” acrosome reaction

Cited by 59 publications

References 25 publications

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

Effects of cryopreservation on the motile sperm subpopulations in semen from Asturiana de los Valles bulls

Cryopreservation of boar sperm comparing different cryoprotectants associated in media based on powdered coconut water, lactose and trehalose

Progesterone significantly enhances the mobility of boar spermatozoa

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