Dynamics of Neutrophiles Phagocitary Activity after Repeated Vaccinations of Laboratory Animals by Experimental Diphtheriae Bacterial Antigen Drug

Yelyseyeva, I. V.; St., Pushkinskaya; Zhdamarova, L. A.; Белозерский, В. И.; Kolpak, S. A.; Balak, O.

doi:10.26693/jmbs05.01.050

Cited by 2 publications

(4 citation statements)

References 18 publications

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“…Its decrease can be the result of both a lack of opsonizing factors in the serum, and defects in the cells themselves. It is known that white blood cells can change their morphological and functional state, taking part in the immune processes of the mammalian body (Yelyseyeva et al, 2020). This ability acts as a criterion for the degree of activity and the nature of the process.…”

Section: вікторія вікторівна лісоваmentioning

confidence: 99%

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2022

Ukrainian journal of veterinary sciences

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Section: вікторія вікторівна лісоваmentioning

confidence: 99%

“…That is why in the mammalian body, neutrophils are both indicators and a universal target for various disorders of homeostasis. Their quantitative and qualitative composition ensures leukocyte activity and is manifested primarily by phagocytic activity (Yelyseyeva et al, 2020).…”

Section: вікторія вікторівна лісоваmentioning

confidence: 99%

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2022

Ukrainian journal of veterinary sciences

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effect of allogeneic blood transfusion on neutrophil functional activity and lymphocyte cytotoxicity in recipient rabbits

Maliuk¹,

Illek²,

Kulida³

et al. 2022

Ukrainian journal of veterinary sciences

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The relevance of this paper is that transfusion of allogeneic blood to recipient animals is always associated with immunological risks. In this regard, the purpose of this study was to assess the state of phagocytic activity of blood neutrophils by indicators of phagocytic index, phagocytic number, and oxygen-dependent bactericidal activity, as well as to establish changes in antibody-dependent cytotoxic activity of lymphocytes in recipient rabbits during allogeneic whole blood transfusion. Modelling of blood transfusions was performed on five clinically healthy rabbits by intravenous administration of whole blood at the rate of 5.5 ml/kg of body weight. Blood samples were taken from animals on Days 3, 7, and 23 after blood transfusion. Neutrophil populations were obtained from blood samples by centrifugation on a double density gradient of 1.077 and 1.093 Ficoll-Verografin. The absorption activity of phagocytes was determined in a microscopic test. To investigate the oxygen-dependent bactericidal activity of neutrophils, a spontaneous test with nitroblue tetrazolium was performed. Antibody-dependent cytotoxic activity of lymphocytes was investigated by colorimetric method. It was found that after the transfusion of whole blood, the phagocytic activity of neutrophils increases with a simultaneous decrease in their absorption capacity. On Days 3 and 7, the results of the spontaneous test with nitroblue tetrazolium decreased. This indicates inactivation of the oxygen-dependent bactericidal activity of neutrophil granulocytes during the first phase of post-transfusion immunological reactions. On Day 23, there was an increase in the values of the indicators of the spontaneous test with nitroblue tetrazolium, which indicates the activation of the bactericidal properties of phagocytes. It was found that on Day 3, the antibody-dependent cytotoxic activity of lymphocytes significantly decreased relative to the initial state, and on Days 7 and 23, it increased. An increase in the antibody-dependent cytotoxic activity of lymphocytes should be associated with the active synthesis of antibodies of the late phase of the immune response. Consequently, transfusion of allogeneic blood causes an immune response in recipient rabbits, without causing immediate and long-term transfusion reactions (changes in heart rate, respiratory rate, body temperature). The obtained results are of practical value both for scientists and practising doctors who use transfusion of whole blood and its components to animals with acute anaemia, impaired functional activity of blood coagulation factors, parasitic, and oncological diseases

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Dynamics of Neutrophiles Phagocitary Activity after Repeated Vaccinations of Laboratory Animals by Experimental Diphtheriae Bacterial Antigen Drug

Cited by 2 publications

References 18 publications

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Untitled

Effect of allogeneic blood transfusion on neutrophil functional activity and lymphocyte cytotoxicity in recipient rabbits

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