Dynamics of organohalide-respiring bacteria and their genes following in-situ chemical oxidation of chlorinated ethenes and biostimulation

Dolinová, Iva; Czinnerová, Marie; Dvořák, Lukáš; Stejskal, Vojtěch; Ševců, Alena; Černík, Miroslav

doi:10.1016/j.chemosphere.2016.05.030

Cited by 27 publications

(8 citation statements)

References 39 publications

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“…Several strains of Dehalococcoides belonging to D. mccartyi or other species have been found to possess one or several dehalogenases that dechlorinate PCE to ethene (i.e. pceA, vcrA, tceA or bvcA) (Dolinová et al, 2016;Zinder, 2016). Sulfurospirillum, known to reduce PCE and TCE (Maillard et al 2011), was found in low abundance in Pz26.…”

Section: Genera Associated With Organohalide Respirationmentioning

confidence: 99%

“…Sulfurospirillum, known to reduce PCE and TCE (Maillard et al 2011), was found in low abundance in Pz26. Strains of Clostridium and Acetobacterium were also shown to dechlorinate CEs in either pure or mixed cultures (Dolinová et al 2016). Sequences assigned to Clostridium were found in low abundance in all wells of the source and plume, while Acetobacterium was detected as one of the most abundant OHR-associated genera in Pz27 and Pz28 (Figure 5).…”

Section: Genera Associated With Organohalide Respirationmentioning

confidence: 99%

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Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

Hellal

Joulian

Urien³

et al. 2021

Science of The Total Environment

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Section: Genera Associated With Organohalide Respirationmentioning

confidence: 99%

Section: Genera Associated With Organohalide Respirationmentioning

confidence: 99%

Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

Hellal

Joulian

Urien³

et al. 2021

Science of The Total Environment

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“…The degree of dechlorination is a value in the range from 0-100 %, when the value 100 % refers to the complete dechlorination of CEs to the non-chlorinated products ethene and ethane [28].…”

Section: Laboratory Analyses Measurements and Calculationsmentioning

confidence: 99%

Cost-Effective Remediation Using Microscale Zvi: Comparison of Commercially Available Products

Pavelková

Stejskal

Vološčuková

et al. 2020

Ecological Chemistry and Engineering S

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Zero-valent iron is very effective in the treatment of groundwater contaminated with chlorinated hydrocarbons and solvents broadly used in industrial production. In terms of its sustainability and cost, a legitimate effort has been devoted to the optimization of the remediation process, which can be demanding and expensive. In this study, the application potential and fundamental properties of several commercial micro-sized zero-valent iron (μZVI) were investigated. Although the manufacturers report the basic parameters of μZVI, it has been shown that the actual reactivity of apparently similar products varies notably. This work was focused on monitoring of frequently occurring contaminants. The actual contaminated water from the Pisecna locality -former landfill of industrial waste, with high levels of chlorinated ethenes and ethanes (PCE, TCE, cis-1,2-DCE and 1,2-DCA) was used for the experiment. The degree of dechlorination reached over 85 % 32 days after the application of μZVI in several samples and a far higher reaction rate for smaller particles was observed. Also, the amount of cis-1,2-DCE, which is characterized by slow decomposition, decreased by more than 95 % over the course of the experiment. Smaller particles showed a much longer sedimentation rate and gradual fractionation was also observed. Monitoring of ORP and pH also suggested that the smaller particles possessed a reduction capacity that was sufficiently high even at the end of the experiment. Laboratory tests with apparently similar μZVI samples indicated considerable differences in their reaction rate and efficiency.

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“…The relative abundance of the 16S rRNA gene (representing total bacterial biomass), Dehalobacter spp., Dehalococcoides spp., Dehalogenimonas spp., Geobacter spp., Desulfitobacterium spp., Gallionella, Geobacter, the VC reductase genes vcrA and bvcA and functional genes encoding enzymes for ethenotroph-mediated aerobic biodegradation [i.e. alkene monooxygenase (etnC) and epoxyalkane:coenzyme M transferase (etnE)] were determined by qPCR, as described previously [29]. Briefly, qPCR reactions were prepared and performed in a LightCycler ® 480 instrument (Roche, Switzerland).…”

Section: Dna Extraction and Qpcrmentioning

confidence: 99%

In situ pilot application of nZVI embedded in activated carbon for remediation of chlorinated ethene-contaminated groundwater: effect on microbial communities

et al. 2020

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Background Nanoscale zero-valent iron (nZVI) is commonly used for remediation of groundwater contaminated by chlorinated ethenes (CEs); however, its long-term reactivity and subsurface transport are limited. A novel nZVI–AC material, consisting of colloidal activated carbon (AC) with embedded nZVI clusters, was developed with the aim of overcoming the limitations of nZVI alone. Results Application of a limited amount of nZVI–AC to an oxic, nitrate-rich, highly permeable quaternary aquifer triggered time-limited transformation of CEs, with noticeable involvement of reductive dechlorination. Reductive dechlorination of CEs was dominantly abiotic, as an increase in the concentration of vinyl chloride (VC) and ethene did not coincide with an increase in the abundance of reductive biomarkers for complete dechlorination of CEs (Dehalococcoides, Dehalogenimonas, VC reductase genes vcrA and bvcA). Application of nZVI–AC under unfavourable hydrochemical conditions resulted in no dramatic change in the microbial community, the reducing effect resulting in temporal proliferation of nitrate and iron reducers only. At a later stage, generation of reduced iron induced an increase in iron-oxidizing bacteria. High concentrations and a continuous mass influx of competing electron acceptors (nitrate and dissolved oxygen) created unfavourable conditions for sulphate-reducers and organohalide-respiring bacteria, though it allowed the survival of aerobic microorganisms of the genera Pseudomonas, Polaromonas and Rhodoferax, known for their ability to assimilate VC or cis-1,2-dichloroethene. A potential for aerobic oxidative degradation of CE metabolites was also indicated by detection of the ethenotroph functional gene etnE. Conclusions This pilot study, based on the application of nZVI–AC, failed to provide a sustainable effect on CE contamination; however, it provided valuable insights into induced hydrogeochemical and microbial processes that could help in designing full-scale applications.

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Dynamics of organohalide-respiring bacteria and their genes following in-situ chemical oxidation of chlorinated ethenes and biostimulation

Cited by 27 publications

References 39 publications

Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

Chlorinated ethene biodegradation and associated bacterial taxa in multi-polluted groundwater: Insights from biomolecular markers and stable isotope analysis

Cost-Effective Remediation Using Microscale Zvi: Comparison of Commercially Available Products

In situ pilot application of nZVI embedded in activated carbon for remediation of chlorinated ethene-contaminated groundwater: effect on microbial communities

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