Dynamics of steroid uptake in rat testis studied by quantitative autoradiography

Frederik, Peter M.; Klepper, D.; Vusse, Ger J.; Molen, H. J. van der

doi:10.1016/0303-7207(76)90075-7

Cited by 10 publications

(1 citation statement)

References 21 publications

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“…(Hansson et al 1974;Mulder et al 1975;, germ cells ), in interstitial tissue (Frederik et al 1976;Verhoeven 1980), and in other cell types (Verhoeven 1980) of rat testis, but the exact location has remained unclear. The development of tech¬ niques for cell separation has provided a means to specify more clearly what cell types are targets for androgen action.…”

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confidence: 98%

Evidence for an androgen receptor in porcine Leydig cells

Isomaa

Orava

Vihko

1987

Acta Endocrinologica

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Cytosol and nuclear androgen receptor concentrations were measured in freshly prepared and cultured Leydig cells of immature pig testis with exchange assays using [3H]methyltrienolone as labelled ligand. Androgen receptors in Leydig cells had high affinity for [3H]methyltrienolone and steroid binding specificity typical of an androgen receptor. The mean receptor concentrations were 76 fmol/mg protein and 210 fmol/mg DNA for cytosol and nuclei, respectively. In sucrose gradients, cytosol androgen receptors sedimented in the 4 S region. The cells maintained androgen receptors under culture conditions. Exposure of cultured cells to [3H]methyltrienolone (10 nmol/l) resulted in accumulation of androgen receptors in the nuclei with maximal uptake by 1 h. We conclude that methyltrienolone binding sites with characteristics of androgen receptors were identified in both cytosol and nuclei of porcine Leydig cells.Androgen receptors have been detected in the rat testis and also in human testicular tissue. Using various biochemical techniques, androgen recep¬ tors have been found in whole testis (Hansson et al. 1974;Mulder et al. 1975), seminiferous tubules Trivial names and systematic names of steroids and abbreviations are: -hydroxy-17ct-methyl-4,9,11-oestratrien-3-one; ORG 2058: 16a-ethyl-21-hydroxy-19-nor-4-pregnene-3,20-dione; DES: diethylstilboestrol, 3,4-bis(p-hydroxyphenyl)-3-hexene; dexamethasone : 9a-fluoro-1 Iß, 17a,21-trihydroxy16a-methyl-1.4-pregnadiene-3,20-dione; DCC : dextran coated charcoal ;EgTA: ethylene glycol-bis-(ß-aminoethylether)-, , ', '-tetra-acetic acid;

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confidence: 98%

Evidence for an androgen receptor in porcine Leydig cells

Isomaa

Orava

Vihko

1987

Acta Endocrinologica

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Autoradiographic detection of mucopolysaccharide accumulation in single fibroblasts

et al. 1977

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A method is described for localizing acid mucopolysaccharides autoradiographically in cultured cells. Normal fibroblasts and fibroblasts, from patients suffering from Mucopolysaccharidosis II disease (MPS II), were cultured for six days in the presence of 35SO4 and one day in unlabelled medium. The cultured cells were transferred to plastic film dish and, after settling, they were rapidly quenched, freeze-dried, fixed in osmium tetroxide vapour and embedded in Epon. Grain counting after autoradiography in 2 mum sections revealed a significant difference (P greater than 0.001) in 35SO4 incorporation in the perinuclear cytoplasm of MPS II cells and control cells grown under the same conditions. Autoradiography was also performed after mixing MPS II cells and control fibroblasts in a ratio 1:1-8 prior to freezing and the same ratio was found between labelled and unlabelled fibroblasts. These results demonstrate the feasibility of the present autoradiographic technique for the detection of the acid mucopolysaccharide storage at the single cell level.

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Androgen receptor in cultured interstitial cells derived from immature rat testis

Verhoeven

1980

Journal of Steroid Biochemistry

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Dynamics of steroid uptake in rat testis studied by quantitative autoradiography

Cited by 10 publications

References 21 publications

Evidence for an androgen receptor in porcine Leydig cells

Evidence for an androgen receptor in porcine Leydig cells

Autoradiographic detection of mucopolysaccharide accumulation in single fibroblasts

Androgen receptor in cultured interstitial cells derived from immature rat testis

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