Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni

Kumari, Neena; Rana, Ritu; Sharma, Y. P.; Kumar, Suresh

doi:10.31018/jans.v8i4.1069

Cited by 4 publications

(7 citation statements)

References 19 publications

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“…Although the changes in the contents of stevioside and rebaudioside A through the different developmental stages were not as drastic as the other steviol glycosides, results obtained from the relative quantification indicated that their contents decrease when the plants entered the flowering stage. The findings from the present study are in agreement with previous reports which stated that the optimal harvesting time for stevia is at the vegetative stage or just before flowering (during the flower bud formation stage), due to the maximum accumulation of steviol glycosides, especially stevioside, rebaudioside A, and rebaudioside C 6,7,40,41 . Considering the fact that the levels of steviol glycosides and also flavonoids are high during the late vegetative stage, i.e., right before budding and during budding, it is reasonable to suggest that these stages are the optimal harvesting times for the local stevia cultivar.…”

Section: Resultssupporting

confidence: 92%

“…This suggested that the content of steviol glycosides was highest during the late vegetative and budding stages and decreased during the flowering stage. The decrease in the content of steviol glycosides could be attributed to the allocation of photoassimilates to the reproductive organs and the reduced transcription of the genes involved in steviol glycoside biosynthesis when the plants enter the flowering stage 5,6 …”

Section: Resultsmentioning

confidence: 99%

“…These factors include the plant organ, geographical origin, growth stage, harvesting time, and extraction procedures. Previously, the dynamic changes of steviol glycosides during the growth cycle of stevia have been investigated 5–7 . However, these studies focused mainly on the changes of major steviol glycosides, namely stevioside and rebaudioside A.…”

Section: Introductionmentioning

confidence: 99%

“…Previously, the dynamic changes of steviol glycosides during the growth cycle of stevia have been investigated. [5][6][7] However, these studies focused mainly on the changes of major steviol glycosides, namely stevioside and rebaudioside A. There is still a lack of information concerning the changes in other metabolites in stevia with respect to the different developmental stages.…”

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confidence: 99%

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LC–MS metabolomics analysis of Stevia rebaudiana Bertoni leaves cultivated in Malaysia in relation to different developmental stages

Lee

Shaari

2021

Phytochemical Analysis

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Introduction Stevia is known for its sweet taste, attributed to the presence of steviol glycosides. Although reports on the dynamic changes of steviol glycosides during development of stevia are available, the data are mainly focused on stevioside and rebaudioside A. Information concerning the comprehensive metabolite profile of stevia in relation to different developmental stages is still lacking. Objective This study investigated the metabolite changes along the developmental stages of a local stevia cultivar. Methodology Stevia leaves were harvested at 4 different developmental stages (early vegetative, late vegetative, budding, and flowering). Samples were then subjected to LC–MS metabolomics analysis to determine the metabolite variations. Results A total of 55 metabolites, comprising phenolic acids, flavonoids, and terpenoids were identified by MS/MS analysis of the stevia leaf extracts, revealing a metabolite profile which was comparatively similar with those of cultivars grown in other countries. PLS‐DA differentiated the early vegetative stage stevia leaf samples from those of the later stages by higher content of phenolic acids. The leaf metabolomes of the later 3 stages (late vegetative, budding, and flowering) were collectively richer in flavonoids. Meanwhile, the content of steviol glycosides is highest during the late vegetative and budding stages. Conclusion The present study provided, for the first time, a general overview of the metabolite variations with regard to the different developmental stages of stevia. The information may facilitate decision making of suitable harvesting times for higher yields of steviol glycosides or a more balanced metabolite profile in terms of pharmacologically useful metabolites.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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LC–MS metabolomics analysis of Stevia rebaudiana Bertoni leaves cultivated in Malaysia in relation to different developmental stages

Lee

Shaari

2021

Phytochemical Analysis

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“…Powdered samples packed in thimbles were extracted with dichloromethane on a water bath using a Soxhlet apparatus for extracting the dichloromethane soluble fraction (Sasidharan et al, 2011). The residual samples in the thimbles (preextracted with dichloromethane) were then extracted with methanol to obtain the sweet compounds of stevioside and rebaudioside-A (Pol et al, 2007;Kumari et al, 2016). Methanol from the methanolic extract was removed by distillation and the sample was dried under vacuum.…”

Section: Extraction Of Stevioside and Rebaudioside-amentioning

confidence: 99%

Interpopulation variations in morphochemical characteristics of Stevia rebaudiana Bertoni

Kumari¹,

Raina²,

Sharma³

2018

Turk J Bot

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Introduction Low-calorie sweeteners have gained importance due to an increase in the number of diabetic people in the world. As per a World Health Organization report, the number of diabetic people will be 80 million by 2025. Synthetic lowcalorie sweeteners have been in use for a long time but are associated with some health risks like carcinogenicity, weight gain, headache, and depression (Tandel, 2011). Consequently, people prefer natural sweeteners rather than synthetic ones. Stevia rebaudiana Bertoni is an important natural sweetener-containing perennial plant of the family Asteraceae (Reis et al., 2017). It is a native of Brazil and Paraguay in South America. In these regions, several other Stevia species such as S. salicifolia Cav., S. lucida Lag., S. rhombifolia Kunth, S. eupatoria Spreng., and S. balansae Hieron. are well known for their ethnopharmacological properties. S. salicifolia Cav. and S. lucida Lag. possess antirheumatic, antihelminthic, and antiinflammatory properties while S. rhombifolia, S. eupatoria, and S. balansae have emetic, antidiarrheal, and diuretic properties, respectively (Ferrazzano et al., 2016). Stevioside and rebaudioside-A are two main sweetener compounds present in the leaves of the plant (Ramesh et al., 2006) and are widely used as zero-calorie sweeteners in many food and beverage industries (Midmore and Rank, 2006; Herranz et al., 2010). These are utilized to sweeten various products like tea (Vanek et al., 2001), seafood, candy, and ice cream (Ozdemir et al., 2015). In addition, the leaves have antidiabetic, antiviral, antiobesity, anticancerous, diuretic, and antiinflammatory properties (Yadav et al., 2011). Stevia rebaudiana grows well under semitropical, subhumid climatic conditions with average annual temperature and rainfall of 25 °C and 1375 mm, respectively (Gupta et al., 2013). The species has been introduced as a commercial crop in several countries, such as the United States, Korea, Tanzania, Brazil, and India (Brandle and Rosa, 1992). According to Ramesh et al. (2006), Stevia rebaudiana performs well at a temperature range from 28 to 40 °C under subtropical Indian conditions. Dwivedi (1999) also reported the cultivation of the crop in humid hilly regions of Assam. The crop is found to grow well in regions like Maharashtra, Rajasthan, Kerala, Orissa, and Punjab in India (Singh et al., 2014). Stevia rebaudiana populations grown from seeds show variations in morphology as well as chemical contents (Tamura et al., 1984; Jadeja et al., 2005). Accordingly, morphochemical characterization needs to be applied to natural sweetener plants like S. rebaudiana. Although earlier studies reported the selection of elite genotypes in natural populations and segregant generations of S. rebaudiana (Abdelsalam et al., 2016; Singh et al., 2017), only limited information is available with regard to the identification of superior plant types in segregant populations. Though the segregating individuals are not needed for crop cultivation as associated with

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Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

Kumari

Kumar

2017

JANS

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Abstract:The Stevia genus encompasses about 200 herbs and shrubs species. Stevia rebaudiana, one of the members has gained commercial importance as a natural low-calorie sweetener, due to the presence of high concentration of stevioside and rebaudioside -A (25% to 45% of stevioside content) in the leaves. The major processes involved in the production and quantification of steviol glycosides are extraction, purification and estimation. Various extraction methods have been used for extraction of steviol glycosides in the world. The extraction methods of steviol glycosides mostly differed at the stage of clarification of extracts. The present study is an attempt to summarize the scattered literature and reports on a single podium. Moreover, it also depicts upto date literature regarding numerous extraction, purification and quantitative estimation methods for steviol glycosides

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Dynamics of steviol glycosides (stevioside and rebaudioside-A) with growth and development of Stevia rebaudiana Bertoni

Cited by 4 publications

References 19 publications

LC–MS metabolomics analysis of Stevia rebaudiana Bertoni leaves cultivated in Malaysia in relation to different developmental stages

LC–MS metabolomics analysis of Stevia rebaudiana Bertoni leaves cultivated in Malaysia in relation to different developmental stages

Interpopulation variations in morphochemical characteristics of Stevia rebaudiana Bertoni

Chemistry and analytical techniques for ent-kaurene-glycosides of Stevia rebaudiana Bertoni - A review

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