Dynamics of supported lipid bilayer deposition from vesicle suspensions

Bucak, Şeyda; Wang, Chen; Laibinis, Paul E.; Hatton, T. Alan

doi:10.1016/j.jcis.2010.04.087

Cited by 11 publications

(6 citation statements)

References 39 publications

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“…In general, receding contact angles give information about liquid sorption/retention and/or liquid penetration into the deposited layer whereas the contact angle hysteresis results from the molecule size of the test liquid [22][23][24], i.e., larger molecules have a lower tendency to penetrate the film layer and have a low hysteresis of the contact angle. When phospholipid molecules are in contact with diiodomethane having an average molecular surface (compared to water) [25], their reorganization is almost impossible.…”

Section: Diiodomethane Contact Angle and Its Hysteresismentioning

confidence: 99%

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Ładniak

Jurak

Wiącek

2019

Colloids and Interfaces

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The investigations were carried out to determine wettability of the 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) monolayers transferred from the liquid subphases containing chitosan (Ch), hyaluronic acid (HA), and/or titanium dioxide (TiO2) to a glass support by means of the Langmuir–Blodgett (LB) technique. For comparative purposes, the analysis of the plates surfaces emerged from the analogous subphases without the phospholipid film was also made. Characterization of the DPPC monolayers was based on the contact angle measurements using three test liquids (water, formamide, diiodomethane) and a simulated body fluid (SBF) solution in which the concentration of ions was close to that of human plasma. After deposition of the DPPC monolayers on the glass plates, a significant increase in the contact angles of all the probe liquids was observed compared to the plates pulled out from the given subphase without floating DPPC. The presence of phospholipid monolayer increased the hydrophobic character of the surface due to orientation of its molecules with hydrocarbon chains towards the air. In addition, the components of the subphase attached along with DPPC to the glass support modify the surface polarity. The largest changes were observed in the presence of TiO2.

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Section: Diiodomethane Contact Angle and Its Hysteresismentioning

confidence: 99%

Wettability of DPPC Monolayers Deposited from the Titanium Dioxide–Chitosan–Hyaluronic Acid Subphases on Glass

Ładniak

Jurak

Wiącek

2019

Colloids and Interfaces

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“…Ca 2+ promotes adsorption of negatively charged liposomes on hydrophilic silica [103] and saturated lipids are needed to achieve a sufficiently high stability in another case [96]. Buffer [104], lipid composition and surface charge [99,105] influence bilayer formation on silica supports. However, the major difficulty remains to keep membrane proteins functional in solid supported lipid bilayers.…”

Section: Lipid Bilayers With Functional Membrane Proteinsmentioning

confidence: 99%

Challenges in the Development of Functional Assays of Membrane Proteins

Tiefenauer

Demarche

2012

Materials

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Lipid bilayers are natural barriers of biological cells and cellular compartments. Membrane proteins integrated in biological membranes enable vital cell functions such as signal transduction and the transport of ions or small molecules. In order to determine the activity of a protein of interest at defined conditions, the membrane protein has to be integrated into artificial lipid bilayers immobilized on a surface. For the fabrication of such biosensors expertise is required in material science, surface and analytical chemistry, molecular biology and biotechnology. Specifically, techniques are needed for structuring surfaces in the micro- and nanometer scale, chemical modification and analysis, lipid bilayer formation, protein expression, purification and solubilization, and most importantly, protein integration into engineered lipid bilayers. Electrochemical and optical methods are suitable to detect membrane activity-related signals. The importance of structural knowledge to understand membrane protein function is obvious. Presently only a few structures of membrane proteins are solved at atomic resolution. Functional assays together with known structures of individual membrane proteins will contribute to a better understanding of vital biological processes occurring at biological membranes. Such assays will be utilized in the discovery of drugs, since membrane proteins are major drug targets.

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“…It is a well known fact that the capture of particles by the mononuclear phagocyte system is less likely if the particles are positively charged, mainly if the surface charge density is high. It must be added that there are proofs of the role played by the valence and radius of ions at the solid support-solution interface in providing structural integrity to the adsorbed phospholipid layer [14], or in determining the quality of the coverage [15].…”

Section: Introductionmentioning

confidence: 99%

“…Investigation appears less abundant in the case of adsorption from solution of phospholipids onto solid surfaces (see, however [5,11,15,18,[28][29][30][31][32][33]). Jackson et al [29] studied adsorption of phospholipids onto glass beads from aqueous dispersions of vesicles formed from DPPC and its mixtures with phosphatidylinositol (PI) at different temperatures.…”

Section: Introductionmentioning

confidence: 99%

“…Temperature has in fact an essential effect on both the adsorption rate and adsorbed amount in the range of gel to liquid-crystalline phase transition. The work by Bucak et al [15] demonstrated that below the transition temperature the gel-like state of the adsorbed phospholipids restricts their lateral and bending movements, hindering the organization of the adsorbed layer and reducing the deposition rate. The results in [29] indicated that the roughness of the surface also influenced the adsorption, as demonstrated by the fact that the samples studied had the same size but different specific surface area.…”

Section: Introductionmentioning

confidence: 99%

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