2014
DOI: 10.1016/j.bbabio.2014.06.003
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Dynamics of the active site architecture in plant-type ferredoxin-NADP+ reductases catalytic complexes

Abstract: Kinetic isotope effects in reactions involving hydride transfer and their temperature dependence are powerful tools to explore dynamics of enzyme catalytic sites. In plant-type ferredoxin-NADP(+) reductases the FAD cofactor exchanges a hydride with the NADP(H) coenzyme. Rates for these processes are considerably faster for the plastidic members (FNR) of the family than for those belonging to the bacterial class (FPR). Hydride transfer (HT) and deuteride transfer (DT) rates for the NADP(+) coenzyme reduction of… Show more

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Cited by 13 publications
(13 citation statements)
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“…G). This type of band is attributed to charge‐transfer interactions between the oxidised flavin isoalloxazine ring and the nicotinamide ring of NADPH, FAD ox ‐NADPH charge‐transfer complexes (CTC) (Batie and Kamin , Tejero et al , Lans et al , Sánchez‐Azqueta et al ). Evolution of absorption in both flavin band‐I and CTC bands in the following spectra indicates flavin reduction by HT without the appearance of other CTC bands.…”
Section: Resultsmentioning
confidence: 99%
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“…G). This type of band is attributed to charge‐transfer interactions between the oxidised flavin isoalloxazine ring and the nicotinamide ring of NADPH, FAD ox ‐NADPH charge‐transfer complexes (CTC) (Batie and Kamin , Tejero et al , Lans et al , Sánchez‐Azqueta et al ). Evolution of absorption in both flavin band‐I and CTC bands in the following spectra indicates flavin reduction by HT without the appearance of other CTC bands.…”
Section: Resultsmentioning
confidence: 99%
“…; Tejero et al , Bortolotti et al , Sánchez‐Azqueta et al , ). Moreover, the reduction of FNRL by NADPH differs with respect to the stabilisation of FNRL CTCs (Tejero et al , Peregrina et al , Sánchez‐Azqueta et al , , Bortolotti et al ). Notably, differences in CTCs stabilisation have also been reported between bacterial‐type FPRs relative to plastidic FNRs, as well as upon mutation of key residues in plastid FNRs (Lans et al , Peregrina et al , Bortolotti et al , Sánchez‐Azqueta et al ).…”
Section: Discussionmentioning
confidence: 99%
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“…Plant-type FNRs are subdivided into plastidic-type (herein FNR) and bacterial-type (herein FPR and including Xcc FPR). The bacterial-types catalyze the electron transfer from NADPH to different electron transfer proteins and are mainly involved in nitrogen fixation and detoxification (including ROS) processes [ 11 , 12 ]. On the contrary, the main function of plastidic-type FNRs is to catalyze the photosynthetic electron transfer from PSI to NADP + to produce NADPH, but they are also able to efficiently provide electrons from NADPH to several electron carrier proteins [ 7 , 8 , 13 , 14 , 15 ].…”
Section: Introductionmentioning
confidence: 99%