Dynamics of the change in the content of estradiol receptors in the nuclear fraction of the hypothalamus of female rats during ontogenesis

Babichev, V. N.; LYu, Ozol'; Peryshkova, T. A.

doi:10.1007/bf01184209

Cited by 1 publication

(1 citation statement)

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“…Aliquots of 25 txl each were sampled each hour, and frozen before the determination of LH and FSH. In another series of experiments, the adenohypophyses from 45 animals were processed as previously described [1], to obtain the nuclear fraction. The following were used as labeled hormones: 1,2,6,7-3H-testosterone (3H-T) and 2,4,6,7-~H-estradiol (3H-E2) with a high specific activity (85-104 Ci/ mM).…”

Section: Methodsmentioning

confidence: 99%

State of hypophyseogonadal system in male rats with diabetes (experimental investigation)

Babichev

Peryshkova

Adamskaya

1994

Neurosci Behav Physiol

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It is well known that the neuroendocrine regulation of the reproductive function in male rats is disturbed in diabetes; however, so far there is more than one opinion regarding the level at which these changes take place. The published data relating to the secretion of gonadotropins are highly contradictory: a decreased [20] or unchanging [8] level of the circulating gonadotropins in the blood has been demonstrated. The majority of investigators are in agreement that a decreased level of androgens is observed in diabetic patients [9,20], which attests to a disturbance in the negative feedback mechanism. Both the hypothalamus and the hypophysis may be the locus of decreased sensitivity to androgens in such animals.In our preceding study we investigated the disturbance of negative feedback at the level of the mediobasal hypothalamus (MBH), and did not find alterations in the concentration of the nuclear receptors of androgens in this region. Since the effect of the sex steroids on the secretion of gonadotropins is mediated through specific receptors, this mechanism apparently remains undamaged at the level of the hypothalamus.In this connection, the state of the hypophyseogonadal system was investigated in the present study in male rats with experimental diabetes. For this purpose, an analysis was made of the sensitivity of the hypophysis to luliberin (LH-RH), and the concentration of the nuclear receptors of the sex hormones in the adenohypophysis that participate in the regulation of the secretion of gonadotropins through the negative feedback mechanism was determined in male rats

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Section: Methodsmentioning

confidence: 99%