Dynamics of the Metabolome of Aliinostoc sp. PMC 882.14 in Response to Light and Temperature Variations

Abstract

Cyanobacteria are microorganisms able to adapt to a wide variety of environmental conditions and abiotic stresses. They produce a large number of metabolites that can participate in the dynamic adaptation of cyanobacteria to a range of different light, temperature, and nutrient conditions. Studying the metabolite profile is one way to understand how the physiological status of cells is related to their adaptive response. In this study, we sought to understand how the diversity and dynamics of the whole metabolome depended on the growth phase and various abiotic factors such as light intensity and temperature. The cyanobacterium, Aliinostoc sp. PMC 882.14, was selected for its large number of biosynthetic gene clusters. One group of cells was grown under normal conditions as a control, while other groups were grown under higher light or temperature. Metabolomes were analyzed by mass spectrometry (qTOF-MS/MS) combined with untargeted analysis to investigate metabolite dynamics, and significant variation was found between exponential and stationary phases, regardless of culture conditions. In the higher light group, the synthesis of several metabolites, including shinorine, was induced while other metabolites, such as microviridins, were synthesized under higher temperature conditions. Among highly regulated metabolites, we observed the presence of mycosporine-like amino acids (MAAs) and variants of somamides, microginins, and microviridins. This study demonstrated the importance of considering the physiological state of cyanobacteria for comparative global metabolomics and studies of the regulatory processes involved in production of specific metabolites. Our results also open up new perspectives on the use of organisms such as cyanobacteria for the targeted production of bioactive metabolites.

Exploring the Relationship between Biosynthetic Gene Clusters and Constitutive Production of Mycosporine-like Amino Acids in Brazilian Cyanobacteria

“…Nodularia spumigena CENA596 and Sphaerospermopsis torques-reginae ITEP-024 were the only strains tested that produced detectable concentrations of constitutive MAAs in the same light condition as the others and without additional UV irradiation, described as inductive of MAA production [ 50 , 51 ]. The link between UV irradiance and MAA production has already been vastly reported in the literature [ 3 , 9 , 10 , 31 , 50 , 52 , 53 , 54 , 55 , 56 , 57 ].…”

The NORMAN Suspect List Exchange (NORMAN-SLE): facilitating European and worldwide collaboration on suspect screening in high resolution mass spectrometry

“…A full breakdown is given in Additional file 4 [236]. Of these 19 articles, 12 can be considered "internal", i.e., articles written by authors involved with the NORMAN-SLE, including 5 articles describing SLE datasets [59,118,149,154,174] and 7 others citing SLE lists [24,142,[237][238][239][240][241], while 7 articles are external [242][243][244][245][246][247][248]. Of the 24 lists cited, 6 lists are cited by external authors: S0 SUSDAT, S13 EUCOSMETICS, S14 KEMIPFAS, S25 OECDPFAS, S46 NTPFASREV19 and S75 CyanoMetDB.…”

Limnospira(Cyanobacteria) chemical fingerprint reveals local molecular adaptation

“…The intracellular metabolite contents of 88 strains (all 93 except L. platensis PMC 1287.21, PMC 1288.21, PMC 1298.21, PMC 1300.21, and PMC 1302.21) were analyzed as previously described 31 . Complete procedure is detailed in supplementary material and methods.…”